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The map label for this gene is queA [H]

Identifier: 148825473

GI number: 148825473

Start: 350133

End: 351224

Strand: Direct

Name: queA [H]

Synonym: CGSHiEE_01850

Alternate gene names: 148825473

Gene position: 350133-351224 (Clockwise)

Preceding gene: 148825472

Following gene: 148825474

Centisome position: 19.31

GC content: 36.9

Gene sequence:

>1092_bases
ATGCGTGTTTCAGACTTTAACTTTGATTTACCCGATGAATTAATTGCTCGTTATCCTAAAACTGATCGTGTTTCTTGTCG
TTTATTACAGTTAAATGGTGAAAACGGAGAAATTTTTCACCGCACTTTTTCTGATGTATTAGATTTAATTGATGAAGGCG
ATTTGTTGATTTTTAATAATACGCGCGTTATTCCTGCAAGAATGTTTGGGCGTAAAGCCAGTGGTGGCAAAATTGAAGTT
TTAGTTGAGAGAATGTTAGATGAACATCGTTTCTTAGCGCATATTCGTTCATCGAAATCACCCAAAGAAGGGGCGGAATT
ATTTTTAGGTGAAGATAAATTAGGCGAAAATAATGGTATTAAAGCAGTAATGAAAGCTCGTCATAGCTCGTTGTTTGAAG
TGGAATTAAGTGATAAATCAACCGCTCTTTTAGATGTGTTGCAAACCATTGGGCATATGCCATTGCCGCCTTATATTGAC
CGCCCAGATGAAGAAGCCGATAAAGAATGTTATCAAACGGTTTATAGTAAAGTACCAGGTGCGGTAGCAGCACCAACGGC
TGGTTTGCATTTTGATGAAAATTTACTTGAAAAACTCAAAGCAAAGGGTGTGAATTTTGAATTTGTTACATTACATGTAG
GCGCAGGAACATTCCAGCCTGTTCGTGTGGAAAATATTGAAGATCACGTAATGCACGCGGAGTATGTGGAAGTTTCTCAA
GAAGTCTGTAATGCGATTATCGCAACAAAAAAAGCGGGCAAACGTGTAATTGCAGTGGGAACTACTTCTGTTCGTTCTAT
TGAAAGTGCGGCGCTTTCTGCGGAAGAATTTGGTAATCCAGATTTAATTGAGCCTTATTTTTCTGATACCTCTATTTTTA
TTTATCCTGGTAAAAAATTCCGAGTGGTGGATTGCTTAATTACTAATTTCCACTTACCTGAAAGCACTTTGATTATGTTA
GTTTCAGCTTTTGCTGGTTATAAAAATACCATGAATGCGTATAAGCAGGCAGTGCAAGAAAAATATCGTTTCTTTAGTTA
TGGCGATGCGATGTTTATTAATAAAAATTCTAATGTGAGAGAGTTGGAATAA

Upstream 100 bases:

>100_bases
TTGCTGGTTATTTTATCTTCCCCGAGGAAAGTTTTTTTCTTGAGATCATTTTCAATTCAAACTAGACTTACCGCCTATTT
TATTTAACCTAAATTTTATT

Downstream 100 bases:

>100_bases
GTTGTTTGACTTGTAATTAGTCAGCACCTACAATAAAGAACAAGAGATGGCGGTAACTATCTCTTGTCGGCATCAACTAC
TAATAGCCAGTGCTACTTTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHSSLFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADKECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKQAVQEKYRFFSYGDAMFINKNSNVRELE

Sequences:

>Translated_363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHSSLFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADKECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKQAVQEKYRFFSYGDAMFINKNSNVRELE
>Mature_363_residues
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNNTRVIPARMFGRKASGGKIEV
LVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGIKAVMKARHSSLFEVELSDKSTALLDVLQTIGHMPLPPYID
RPDEEADKECYQTVYSKVPGAVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKFRVVDCLITNFHLPESTLIML
VSAFAGYKNTMNAYKQAVQEKYRFFSYGDAMFINKNSNVRELE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=70.5056179775281, Blast_Score=523, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40739; Mature: 40739

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKIEVLVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGI
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCH
KAVMKARHSSLFEVELSDKSTALLDVLQTIGHMPLPPYIDRPDEEADKECYQTVYSKVPG
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHH
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKF
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCE
RVVDCLITNFHLPESTLIMLVSAFAGYKNTMNAYKQAVQEKYRFFSYGDAMFINKNSNVR
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
ELE
CCC
>Mature Secondary Structure
MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKIEVLVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGI
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCH
KAVMKARHSSLFEVELSDKSTALLDVLQTIGHMPLPPYIDRPDEEADKECYQTVYSKVPG
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHH
EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKF
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCE
RVVDCLITNFHLPESTLIMLVSAFAGYKNTMNAYKQAVQEKYRFFSYGDAMFINKNSNVR
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
ELE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA