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The map label for this gene is djlA [H]

Identifier: 148825450

GI number: 148825450

Start: 319469

End: 320401

Strand: Direct

Name: djlA [H]

Synonym: CGSHiEE_01700

Alternate gene names: 148825450

Gene position: 319469-320401 (Clockwise)

Preceding gene: 148825449

Following gene: 148825456

Centisome position: 17.62

GC content: 40.3

Gene sequence:

>933_bases
GTGCGTGTTATTCTTGCGCCTATGCAAGGGGTTTTAGATCCCTTTGTACGTGAACTTCTCACCGAAGTGAATGACTACGA
TTTATGTATAACAGAATTTGTTCGCGTAGTTGATCAACTTCTTCCTGAAAAAGTATTTTATCGTTTATGCCCTGAATTAA
AAAATCAGGGCTTTACTTCTTCTAGCACGCCTGTGCGAGTGCAGTTGCTAGGGCAGCATCCAGAATGCCTTGCTGAAAAT
GCAATTCGTGCAATCGAGCTTGGCTCTCATGGCATTGATTTAAATTGTGGTTGCCCTTCTAAAACAGTGAATGGCAGCAA
TGGCGGTGCGGCATTATTGAAACAGCCTGAATTGATTTATCGTGCAACTCAAGCCTTACGCAGAGCCGTGCCAAGTGAAT
TCCCCGTTAGTGTAAAAGTGCGGTTAGGCTGGGATGATATTTCTCAAGCTTTTGAAATCGCTGATGCGGTAGAGCAAGGG
GGAGCAACCGAAATTACAGTTCATGGACGCACAAAATCTGATGGTTATCGTGCTGATAGAATTAATTGGAAAAAAATTGG
TAAAGTCCGAGAGCGTTTATCCATTCCTGTTATTGCTAACGGAGAAATTTGGCATTGGCAAGATGGGCAAGATTGCTTAT
CTCAAACAGGTTGTCAGGATTTAATGGTGGGACGAGGTGCGTTGAATATTCCGAATTTAAGTCATGTTCTAAAATCAAAT
GCAGAAAAAATGCCTTGGCGTGAGATTCAAAAAATCTTGCAAAAATACGCTAAGGTCGAAAATGAATATGACAGCGGTTT
TTATCACGTGGCACGAATTAAACAATGGTTACGTTATTTGAATAAGGAATATGATGAGGCGAACCAAGAGTTTGATAAGA
TTAAGACTTGCCAAAATGCTGAAGATTTGAAATTACGTTTAAATGATAAATAA

Upstream 100 bases:

>100_bases
CGAAAGGTTTACCGCCAGAAATGATGGAAATGGCAAAAGAAAAAACTCAACAGATTCAAGCCGCTTACGATTTAATTTGT
AAAGCAAAAGGCTGGAAATA

Downstream 100 bases:

>100_bases
AAAACCTGCTAATCAGCAGGTTTTCTTTTTATAAATTATTTAAAAACTCACCGCACTTTTTAATTTTTTCAGTGCATTGG
TTTCTAATTGACGGATACGT

Product: DnaJ-like membrane chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSSTPVRVQLLGQHPECLAEN
AIRAIELGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKSDGYRADRINWKKIGKVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWREIQKILQKYAKVENEYDSGFYHVARIKQWLRYLNKEYDEANQEFDKIKTCQNAEDLKLRLNDK

Sequences:

>Translated_310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSSTPVRVQLLGQHPECLAEN
AIRAIELGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKSDGYRADRINWKKIGKVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWREIQKILQKYAKVENEYDSGFYHVARIKQWLRYLNKEYDEANQEFDKIKTCQNAEDLKLRLNDK
>Mature_310_residues
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTSSSTPVRVQLLGQHPECLAEN
AIRAIELGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQG
GATEITVHGRTKSDGYRADRINWKKIGKVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
AEKMPWREIQKILQKYAKVENEYDSGFYHVARIKQWLRYLNKEYDEANQEFDKIKTCQNAEDLKLRLNDK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=243, Percent_Identity=31.2757201646091, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=321, Percent_Identity=27.7258566978193, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI8923374, Length=268, Percent_Identity=27.2388059701493, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI239788483, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI239788462, Length=177, Percent_Identity=28.2485875706215, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=303, Percent_Identity=67.3267326732673, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1789660, Length=305, Percent_Identity=28.8524590163934, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=325, Percent_Identity=23.6923076923077, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=273, Percent_Identity=30.03663003663, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=197, Percent_Identity=33.502538071066, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=171, Percent_Identity=29.8245614035088, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=207, Percent_Identity=27.536231884058, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=165, Percent_Identity=27.8787878787879, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921524, Length=220, Percent_Identity=32.7272727272727, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=29.2397660818713, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=29.2397660818713, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=31.6666666666667, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35125; Mature: 35125

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTS
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SSTPVRVQLLGQHPECLAENAIRAIELGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIY
CCCCEEEEEECCCHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCHHHHCCHHHHH
RATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQGGATEITVHGRTKSDGYRADR
HHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
INWKKIGKVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
CCHHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCC
AEKMPWREIQKILQKYAKVENEYDSGFYHVARIKQWLRYLNKEYDEANQEFDKIKTCQNA
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDLKLRLNDK
CCEEEEECCC
>Mature Secondary Structure
MRVILAPMQGVLDPFVRELLTEVNDYDLCITEFVRVVDQLLPEKVFYRLCPELKNQGFTS
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SSTPVRVQLLGQHPECLAENAIRAIELGSHGIDLNCGCPSKTVNGSNGGAALLKQPELIY
CCCCEEEEEECCCHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCHHHHCCHHHHH
RATQALRRAVPSEFPVSVKVRLGWDDISQAFEIADAVEQGGATEITVHGRTKSDGYRADR
HHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
INWKKIGKVRERLSIPVIANGEIWHWQDGQDCLSQTGCQDLMVGRGALNIPNLSHVLKSN
CCHHHHHHHHHHHCCCEEECCCEEECCCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCC
AEKMPWREIQKILQKYAKVENEYDSGFYHVARIKQWLRYLNKEYDEANQEFDKIKTCQNA
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDLKLRLNDK
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]