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The map label for this gene is yfiF [C]

Identifier: 148825296

GI number: 148825296

Start: 167556

End: 168260

Strand: Direct

Name: yfiF [C]

Synonym: CGSHiEE_00880

Alternate gene names: 148825296

Gene position: 167556-168260 (Clockwise)

Preceding gene: 148825295

Following gene: 148825298

Centisome position: 9.24

GC content: 41.28

Gene sequence:

>705_bases
ATGAAGGTTTACGGTGAAAATGCCTGTTTAGCTTTATTTGCAGAACGCCCTGAAAGTATCGTTCGATTGTGGGCAACCGT
GCAAATGTCACACAAAATTGGCGAGGTATTGAGTTATTTAGCCGAAAACAAGAAGGCTTATCACGTTGTAGATAGCGAAG
AATTAGCTAGAGTGAGCGGAACGGAACATCATGGTGGCATTTGCTTATTAGTGAAGAAACCGCGAGCTTTCACGTTGCAA
GGATATTTGGATATTCCTCGTAACGAGGATTGTTTAGTGCTATTAGACAACGTGAATAATGCACAAAATATTGGTGGCGT
ATTGCGTACTTGTGCTTATTTTGGCGTGAAAAATATTGTCGCCGATAATGTGGAAAATCTGTATTCAGCAGCCTCAATGC
GTGTAGCAGAAGGTGGCGCAGAATATATTCGTGTGTTAGAAACGGATGATATTGATTCGGCTTTAATGCAATTGCGTAAA
TCAGGCTATCAAGTTATTCACGTATCGCACAATAAACAAGGCGACCCATTGGATAAAGTGCGGTTAAAAAATAAAGTGGT
TTTTGTGCTAAGTGAAAGTTCTACGGAAAGTTTAGCGACGCCAGAAGACACACAAGTGCGTTTAACGCTCGCAAGCCCAA
TTAAATCAGGCTTAAACATTGCAGTGAATGCGGGTGTGTTGTTGGCGAAGTGGTATTTTAGATAG

Upstream 100 bases:

>100_bases
ATAAAAACGACGGGCACAATTTATAAAACAAAAGAAAAGAAAAACGGGTGCACTTTCTCCTCGTGCGCCTGAAAAAATTA
AGAAAAACCGTGCGGAAGAA

Downstream 100 bases:

>100_bases
TGTATGATTAAAGGGGTAAATGATTTCCCCTTTAAAATTTCAAATAAAAGTCTTTAGTCAATTCAATAAACGCTTCAGTG
TATTGATTATCCGCATCATA

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQ
GYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMQLRK
SGYQVIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR

Sequences:

>Translated_234_residues
MKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQ
GYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMQLRK
SGYQVIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR
>Mature_234_residues
MKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSGTEHHGGICLLVKKPRAFTLQ
GYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIVADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMQLRK
SGYQVIHVSHNKQGDPLDKVRLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=234, Percent_Identity=43.5897435897436, Blast_Score=207, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25863; Mature: 25863

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSG
CCEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCC
TEHHGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIV
CCCCCCEEEEEECCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMQLRKSGYQVIHVSHNKQGDPLDKV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHE
RLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR
EECCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEEECC
>Mature Secondary Structure
MKVYGENACLALFAERPESIVRLWATVQMSHKIGEVLSYLAENKKAYHVVDSEELARVSG
CCEECCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCC
TEHHGGICLLVKKPRAFTLQGYLDIPRNEDCLVLLDNVNNAQNIGGVLRTCAYFGVKNIV
CCCCCCEEEEEECCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ADNVENLYSAASMRVAEGGAEYIRVLETDDIDSALMQLRKSGYQVIHVSHNKQGDPLDKV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHE
RLKNKVVFVLSESSTESLATPEDTQVRLTLASPIKSGLNIAVNAGVLLAKWYFR
EECCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]