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The map label for this gene is ispB [C]

Identifier: 148824590

GI number: 148824590

Start: 3808554

End: 3809606

Strand: Reverse

Name: ispB [C]

Synonym: TBFG_13417

Alternate gene names: 148824590

Gene position: 3809606-3808554 (Counterclockwise)

Preceding gene: 148824591

Following gene: 148824589

Centisome position: 86.1

GC content: 61.35

Gene sequence:

>1053_bases
ATGGGAGGCGTATTGACTCTGGATGCGGCTTTTCTGGGGAGTGTCCCTGCTGACCTTGGGAAAGCATTGTTAGAACGGGC
TCGAGCGGATTGTGGCCCTGTGCTGCATCGGGCCATTGAGTCGATGCGCGAGCCGTTGGCGACGATGGCGGGATATCACC
TTGGCTGGTGGAACGCCGATCGATCCACAGCGGCAGGATCGTCGGGCAAGTATTTTCGCGCCGCCCTTGTCTACGCGGCG
GCCGCGGCTTGCGGTGGTGATGTCGGTGATGCTACTCCGGTGTCGGCGGCTGTGGAGTTGGTGCACAACTTCACGTTGTT
GCATGACGATGTGATGGATGGGGATGCGACTCGCAGGGGACGGCCGACGGTATGGAGTGTGTGGGGTGTGGGCGGCGCGA
TCCTGTTGGGAGATGCGTTGCACGCCACGGCCGTTCGGATACTGACCGGCCTGACCGACGAGTGTGTGGCGGTCAGGGCG
ATAAGGCGATTGCAGATGTCGTGCCTGGACTTGTGTATCGGCCAGTTTGAGGATTGTTTATTGGAGGGACAGCCCGAAGT
TACAGTCGATGACTATCTGCGGATGGCCGCGGGCAAGACGGCCGCCCTGACCGGTTGTTGTTGCGCTCTGGGAGCTTTAG
TGGCCAATGCAGACGACGCGACGATTGCTGCGTTGGAGCGTTTTGGCCATGAGTTGGGTCTTGCGTTTCAATGCGTCGAC
GATCTGATCGGCATCTGGGGTGACCCTGGTGTGACGGGAAAGCCGGTCGGCAACGACCTTGCCCGACGTAAGGCGACACT
GCCAGTTGTTGCCGCCCTGAACTCGCGATCCGAAGCGGCGACCGAATTGGCGGCCCTCTATCAAGCACCTGCCGCAATGA
CAGCAAGCGATGTCGAGCGGGCTACCGCGCTTGTCAAGGTGGCCGGGGGTGGTCATGTGGCGCAGCGATGCGCCGATGAA
CGCATCCAGGCTGCTATTGCGGCACTGCCCGATGCGGTGCGATCCCCAGACCTGATCGCGTTGTCGCAACTGATATGTCG
CCGGGAGTGCTGA

Upstream 100 bases:

>100_bases
TTGATATTTGAAATATTTGGGTATCTCCGAAGTGAAGCAATATCGCCGATTGCTCGTTCGCTGAAAATAGCGACTCACTG
TTGCTCCGGTGAGGTTAAGA

Downstream 100 bases:

>100_bases
TGGCTGAGGTGTTCGTGGGACCGGTCGCACAGGGATACGCTTCGGGTGAAGTCACGGTGCTGTTGGCGTCGCCGCGGTCG
TTTTGCGCCGGTGTAGAGCG

Product: polyprenyl synthetase idsB

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGGVLTLDAAFLGSVPADLGKALLERARADCGPVLHRAIESMREPLATMAGYHLGWWNADRSTAAGSSGKYFRAALVYAA
AAACGGDVGDATPVSAAVELVHNFTLLHDDVMDGDATRRGRPTVWSVWGVGGAILLGDALHATAVRILTGLTDECVAVRA
IRRLQMSCLDLCIGQFEDCLLEGQPEVTVDDYLRMAAGKTAALTGCCCALGALVANADDATIAALERFGHELGLAFQCVD
DLIGIWGDPGVTGKPVGNDLARRKATLPVVAALNSRSEAATELAALYQAPAAMTASDVERATALVKVAGGGHVAQRCADE
RIQAAIAALPDAVRSPDLIALSQLICRREC

Sequences:

>Translated_350_residues
MGGVLTLDAAFLGSVPADLGKALLERARADCGPVLHRAIESMREPLATMAGYHLGWWNADRSTAAGSSGKYFRAALVYAA
AAACGGDVGDATPVSAAVELVHNFTLLHDDVMDGDATRRGRPTVWSVWGVGGAILLGDALHATAVRILTGLTDECVAVRA
IRRLQMSCLDLCIGQFEDCLLEGQPEVTVDDYLRMAAGKTAALTGCCCALGALVANADDATIAALERFGHELGLAFQCVD
DLIGIWGDPGVTGKPVGNDLARRKATLPVVAALNSRSEAATELAALYQAPAAMTASDVERATALVKVAGGGHVAQRCADE
RIQAAIAALPDAVRSPDLIALSQLICRREC
>Mature_349_residues
GGVLTLDAAFLGSVPADLGKALLERARADCGPVLHRAIESMREPLATMAGYHLGWWNADRSTAAGSSGKYFRAALVYAAA
AACGGDVGDATPVSAAVELVHNFTLLHDDVMDGDATRRGRPTVWSVWGVGGAILLGDALHATAVRILTGLTDECVAVRAI
RRLQMSCLDLCIGQFEDCLLEGQPEVTVDDYLRMAAGKTAALTGCCCALGALVANADDATIAALERFGHELGLAFQCVDD
LIGIWGDPGVTGKPVGNDLARRKATLPVVAALNSRSEAATELAALYQAPAAMTASDVERATALVKVAGGGHVAQRCADER
IQAAIAALPDAVRSPDLIALSQLICRREC

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=292, Percent_Identity=27.7397260273973, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789578, Length=286, Percent_Identity=27.6223776223776, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786623, Length=226, Percent_Identity=33.6283185840708, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=255, Percent_Identity=30.1960784313725, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508563, Length=286, Percent_Identity=26.5734265734266, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651612, Length=238, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 36390; Mature: 36259

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGVLTLDAAFLGSVPADLGKALLERARADCGPVLHRAIESMREPLATMAGYHLGWWNAD
CCCEEEEHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
RSTAAGSSGKYFRAALVYAAAAACGGDVGDATPVSAAVELVHNFTLLHDDVMDGDATRRG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPTVWSVWGVGGAILLGDALHATAVRILTGLTDECVAVRAIRRLQMSCLDLCIGQFEDCL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGQPEVTVDDYLRMAAGKTAALTGCCCALGALVANADDATIAALERFGHELGLAFQCVD
HCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DLIGIWGDPGVTGKPVGNDLARRKATLPVVAALNSRSEAATELAALYQAPAAMTASDVER
HHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
ATALVKVAGGGHVAQRCADERIQAAIAALPDAVRSPDLIALSQLICRREC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GGVLTLDAAFLGSVPADLGKALLERARADCGPVLHRAIESMREPLATMAGYHLGWWNAD
CCEEEEHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
RSTAAGSSGKYFRAALVYAAAAACGGDVGDATPVSAAVELVHNFTLLHDDVMDGDATRRG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPTVWSVWGVGGAILLGDALHATAVRILTGLTDECVAVRAIRRLQMSCLDLCIGQFEDCL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGQPEVTVDDYLRMAAGKTAALTGCCCALGALVANADDATIAALERFGHELGLAFQCVD
HCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DLIGIWGDPGVTGKPVGNDLARRKATLPVVAALNSRSEAATELAALYQAPAAMTASDVER
HHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
ATALVKVAGGGHVAQRCADERIQAAIAALPDAVRSPDLIALSQLICRREC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]