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The map label for this gene is ligB

Identifier: 148824254

GI number: 148824254

Start: 3437348

End: 3438871

Strand: Direct

Name: ligB

Synonym: TBFG_13079

Alternate gene names: 148824254

Gene position: 3437348-3438871 (Clockwise)

Preceding gene: 148824251

Following gene: 148824255

Centisome position: 77.69

GC content: 67.98

Gene sequence:

>1524_bases
GTGCTCCTTCACGACGTGGCCATCACCTCAATGGACGTGGCCGCCACCTCGTCGCGGCTGACCAAGGTCGCGCGCATCGC
CGCCCTGTTGCACCGCGCCGCGCCAGACACACAGCTGGTCACGATCATCGTGTCGTGGCTCTCCGGCGAGCTGCCGCAAC
GCCATATCGGTGTCGGGTGGGCGGCATTGCGGTCCCTACCGCCGCCCGCGCCGCAACCGGCGTTGACCGTCACCGGTGTC
GACGCCACCCTCTCTAAGATCGGCACTCTATCGGGCAAAGGGTCTCAGGCGCAGCGCGCGGCACTCGTTGCGGAATTGTT
CTCCGCCGCAACCGAAGCTGAGCAAACCTTTTTGTTGCGACTGCTCGGCGGTGAACTGCGCCAGGGCGCAAAGGGCGGGA
TCATGGCCGATGCGGTCGCCCAGGCCGCCGGGCTCCCGGCCGCGACGGTCCAACGCGCCGCGATGCTAGGCGGCGACCTG
GCGGCAGCGGCGGCGGCCGGCCTGTCCGGCGCGGCGCTGGACACCTTCACCCTGCGAGTGGGCCGACCGATAGGCCCGAT
GCTGGCACAGACCGCGACCAGCGTCCATGATGCACTCGAACGTCACGGCGGCACAACCATTTTCGAGGCTAAACTAGACG
GCGCGCGAGTGCAGATCCACCGGGCAAACGACCAGGTCAGGATCTACACCCGAAGCCTGGACGACGTCACTGCCCGGCTG
CCCGAGGTGGTGGAGGCAACACTGGCACTGCCGGTCCGGGATCTAGTGGCCGACGGCGAGGCGATCGCGCTGTGCCCGGA
CAACCGGCCGCAGCGTTTCCAGGTCACCGCATCACGGTTCGGCCGATCGGTCGATGTTGCGGCTGCCCGCGCGACGCAGC
CACTTTCGGTGTTCTTCTTCGACATCCTGCATCGGGATGGTACCGACTTGCTCGAAGCGCCGACCACCGAGCGGCTGGCC
GCCCTGGACGCACTGGTGCCGGCTCGGCACCGCGTGGACCGGCTGATCACGTCCGATCCAACGGACGCGGCCAACTTCCT
GGATGCGACGCTGGCCGCCGGCCACGAGGGGGTGATGGCCAAGGCACCGGCCGCTCGTTACCTTGCGGGTCGCCGCGGAG
CGGGCTGGCTGAAGGTCAAGCCGGTGCACACACTCGACTTGGTGGTGCTCGCGGTGGAATGGGGCTCGGGACGCCGGCGC
GGCAAGCTCTCGAATATTCACCTGGGCGCACGCGATCCGGCTACCGGTGGATTCGTGATGGTGGGCAAGACCTTCAAAGG
AATGACCGACGCCATGCTGGACTGGCAGACCACCAGGTTTCACGAGATCGCGGTGGGTCCGACAGACGGCTACGTCGTCC
AACTTAGGCCCGAGCAGGTGGTCGAGGTAGCCCTCGACGGCGTGCAAAGGTCGTCGCGCTACCCGGGCGGGCTGGCATTG
CGGTTTGCCCGCGTGGTGCGCTACCGCGCCGACAAGGACCCGGCCGAGGCCGACACCATCGATGCCGTGCGCGCGCTCTA
CTGA

Upstream 100 bases:

>100_bases
GAGTATACTAATTGTCTGTCGCGGCCGCGAGACACGGCTTGCTCGGGCACGCCAGCCTTGCCCTCGCCAACGATGTCGGC
GAGACATGCCAAGCTGAACC

Downstream 100 bases:

>100_bases
TCGCACGGCGAGAGTGACTCCTGCGACGGGACACGCCGGCTGGGCGTCGCCAGATTCACGCTCGTCGACCAAGCGGGCGG
GACAAGCAGCTGCAAGGATC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLSGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY

Sequences:

>Translated_507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLSGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY
>Mature_507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLSGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=507, Percent_Identity=28.0078895463511, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI46255052, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI23199993, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI148539894, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71985269, Length=496, Percent_Identity=26.6129032258064, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320038, Length=522, Percent_Identity=28.1609195402299, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24762424, Length=506, Percent_Identity=26.6798418972332, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI85815841, Length=501, Percent_Identity=25.3493013972056, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_MYCBO (Q7TTR7)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856734.1
- HSSP:   P71571
- ProteinModelPortal:   Q7TTR7
- SMR:   Q7TTR7
- EnsemblBacteria:   EBMYCT00000016661
- GeneID:   1093555
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3089
- GeneTree:   EBGT00050000016312
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- ProtClustDB:   PRK03180
- BioCyc:   MBOV233413:MB3089-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 53727; Mature: 53727

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 211-211 BINDING 209-209 BINDING 216-216 BINDING 231-231 BINDING 260-260 BINDING 300-300 BINDING 372-372 BINDING 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCHH
AALRSLPPPAPQPALTVTGVDATLSKIGTLSGKGSQAQRAALVAELFSAATEAEQTFLLR
HHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRV
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEC
GRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHH
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFF
HHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCEEHHHHHCCCCHHHHHH
DILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMA
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
KAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVM
CCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
VGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
ECCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
RFARVVRYRADKDPAEADTIDAVRALY
HHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCHH
AALRSLPPPAPQPALTVTGVDATLSKIGTLSGKGSQAQRAALVAELFSAATEAEQTFLLR
HHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRV
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEC
GRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHH
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFF
HHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCEEHHHHHCCCCHHHHHH
DILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMA
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
KAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVM
CCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
VGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
ECCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
RFARVVRYRADKDPAEADTIDAVRALY
HHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972