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The map label for this gene is lip2 [H]

Identifier: 148824160

GI number: 148824160

Start: 3336046

End: 3337176

Strand: Reverse

Name: lip2 [H]

Synonym: TBFG_12985

Alternate gene names: 148824160

Gene position: 3337176-3336046 (Counterclockwise)

Preceding gene: 148824162

Following gene: 148824159

Centisome position: 75.43

GC content: 65.34

Gene sequence:

>1131_bases
ATGACCAAGAGTCTGCCAGGTGTGGCAGACCTCCGTCTCGGAGCAAACCACCCGCGCATGTGGACACGGCGTGTGCAGGG
CACCGTCGTCAACGTCGGAGTCAAGGTCCTTCCGTGGATCCCGACGCCTGCCAAACGGATCCTGTCGGCCGGCCGATCGG
TCATTATCGATGGCAATACGCTTGACCCCACCCTGCAGCTGATGTTGTCGACTTCGCGGATCTTCGGTGTCGACGGCCTG
GCCGTAGACGACGACATCGTCGCCTCTCGCGCGCACATGCGCGCGATATGCGAGGCGATGCCCGGTCCGCAGATCCACGT
CGACGTGACCGACCTGTCAATACCCGGACCAGCCGGTGAGATCCCGGCGCGGCACTACCGCCCCAGCGGCGGTGGCGCCA
CGCCGCTGCTGGTCTTTTACCACGGTGGCGGCTGGACGCTCGGGGACTTGGACACCCATGACGCGCTGTGCCGGTTGACC
TGTCGCGACGCCGACATCCAGGTACTGTCGATCGACTACCGGTTGGCTCCCGAGCATCCGGCGCCGGCCGCGGTTGAAGA
TGCCTATGCGGCTTTTGTATGGGCCCATGAGCACGCTTCCGATGAGTTCGGTGCGCTCCCCGGGCGGGTCGCGGTGGGCG
GGGACAGTGCGGGCGGCAACCTGTCGGCCGTGGTGTGTCAGTTGGCGCGCGACAAGGCTCGCTACGAGGGTGGGCCCACC
CCGGTGCTGCAGTGGCTGCTGTATCCCAGGACCGATTTCACCGCACAGACTCGCTCAATGGGCCTGTTCGGCAATGGCTT
CCTGCTGACCAAGCGGGACATCGATTGGTTCCACACGCAATACCTCAGGGATTCCGATGTCGATCCGGCGGATCCGCGGT
TGTCGCCGTTGTTGGCCGAATCGCTGTCCGGGCTGGCGCCTGCTCTCATCGCGGTCGCCGGCTTCGACCCATTGCGCGAC
GAAGGAGAAAGCTATGCGAAGGCGCTGCGGGCTGCCGGGACTGCAGTGGACTTGCGGTATCTGGGTTCGCTGACGCATGG
CTTCCTCAACCTGTTTCAGCTGGGGGGAGGCAGTGCTGCCGGGACCAACGAGCTGATCTCCGCGCTGCGCGCGCACCTTA
GCCGGGTTTGA

Upstream 100 bases:

>100_bases
TTGCCCGGTGTCGGCCGGCAGGACCCGAAAGACATCCAGCCTGACGGCGCGGATCGAGTGTCCGTTGCTGCGCGTGACAG
AGTTTTGTAGGGAGCAGCCC

Downstream 100 bases:

>100_bases
GTCGAGGCTGGCTTGCCGCCGGTACGCTAGAGGCGCCGTCGCCTGACCGCGGCCGCGACAAGCCGACAAATCGAGCACGT
GAGGATCAGCGAAACTGTGG

Product: lipase/esterase lipN

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKSLPGVADLRLGANHPRMWTRRVQGTVVNVGVKVLPWIPTPAKRILSAGRSVIIDGNTLDPTLQLMLSTSRIFGVDGL
AVDDDIVASRAHMRAICEAMPGPQIHVDVTDLSIPGPAGEIPARHYRPSGGGATPLLVFYHGGGWTLGDLDTHDALCRLT
CRDADIQVLSIDYRLAPEHPAPAAVEDAYAAFVWAHEHASDEFGALPGRVAVGGDSAGGNLSAVVCQLARDKARYEGGPT
PVLQWLLYPRTDFTAQTRSMGLFGNGFLLTKRDIDWFHTQYLRDSDVDPADPRLSPLLAESLSGLAPALIAVAGFDPLRD
EGESYAKALRAAGTAVDLRYLGSLTHGFLNLFQLGGGSAAGTNELISALRAHLSRV

Sequences:

>Translated_376_residues
MTKSLPGVADLRLGANHPRMWTRRVQGTVVNVGVKVLPWIPTPAKRILSAGRSVIIDGNTLDPTLQLMLSTSRIFGVDGL
AVDDDIVASRAHMRAICEAMPGPQIHVDVTDLSIPGPAGEIPARHYRPSGGGATPLLVFYHGGGWTLGDLDTHDALCRLT
CRDADIQVLSIDYRLAPEHPAPAAVEDAYAAFVWAHEHASDEFGALPGRVAVGGDSAGGNLSAVVCQLARDKARYEGGPT
PVLQWLLYPRTDFTAQTRSMGLFGNGFLLTKRDIDWFHTQYLRDSDVDPADPRLSPLLAESLSGLAPALIAVAGFDPLRD
EGESYAKALRAAGTAVDLRYLGSLTHGFLNLFQLGGGSAAGTNELISALRAHLSRV
>Mature_375_residues
TKSLPGVADLRLGANHPRMWTRRVQGTVVNVGVKVLPWIPTPAKRILSAGRSVIIDGNTLDPTLQLMLSTSRIFGVDGLA
VDDDIVASRAHMRAICEAMPGPQIHVDVTDLSIPGPAGEIPARHYRPSGGGATPLLVFYHGGGWTLGDLDTHDALCRLTC
RDADIQVLSIDYRLAPEHPAPAAVEDAYAAFVWAHEHASDEFGALPGRVAVGGDSAGGNLSAVVCQLARDKARYEGGPTP
VLQWLLYPRTDFTAQTRSMGLFGNGFLLTKRDIDWFHTQYLRDSDVDPADPRLSPLLAESLSGLAPALIAVAGFDPLRDE
GESYAKALRAAGTAVDLRYLGSLTHGFLNLFQLGGGSAAGTNELISALRAHLSRV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=319, Percent_Identity=26.6457680250784, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI68299767, Length=294, Percent_Identity=27.2108843537415, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI206597554, Length=305, Percent_Identity=27.5409836065574, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI61966717, Length=323, Percent_Identity=24.7678018575851, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI21328446, Length=90, Percent_Identity=37.7777777777778, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786682, Length=239, Percent_Identity=32.2175732217573, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17567059, Length=300, Percent_Identity=30.6666666666667, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17540028, Length=133, Percent_Identity=35.3383458646617, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI72001146, Length=150, Percent_Identity=36.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=155, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 40135; Mature: 40004

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLPGVADLRLGANHPRMWTRRVQGTVVNVGVKVLPWIPTPAKRILSAGRSVIIDGNT
CCCCCCCCEEEEECCCCCHHHHHHHCCEEEEECEEEEECCCCHHHHHHHCCCEEEEECCC
LDPTLQLMLSTSRIFGVDGLAVDDDIVASRAHMRAICEAMPGPQIHVDVTDLSIPGPAGE
CCHHHHHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
IPARHYRPSGGGATPLLVFYHGGGWTLGDLDTHDALCRLTCRDADIQVLSIDYRLAPEHP
CCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHEEEEECCCCEEEEEEEEEECCCCC
APAAVEDAYAAFVWAHEHASDEFGALPGRVAVGGDSAGGNLSAVVCQLARDKARYEGGPT
CCCHHHHHHEEEEEEECCCCCHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCH
PVLQWLLYPRTDFTAQTRSMGLFGNGFLLTKRDIDWFHTQYLRDSDVDPADPRLSPLLAE
HHHHHHHCCCCCCCHHHHCCCEECCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHH
SLSGLAPALIAVAGFDPLRDEGESYAKALRAAGTAVDLRYLGSLTHGFLNLFQLGGGSAA
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
GTNELISALRAHLSRV
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKSLPGVADLRLGANHPRMWTRRVQGTVVNVGVKVLPWIPTPAKRILSAGRSVIIDGNT
CCCCCCCEEEEECCCCCHHHHHHHCCEEEEECEEEEECCCCHHHHHHHCCCEEEEECCC
LDPTLQLMLSTSRIFGVDGLAVDDDIVASRAHMRAICEAMPGPQIHVDVTDLSIPGPAGE
CCHHHHHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
IPARHYRPSGGGATPLLVFYHGGGWTLGDLDTHDALCRLTCRDADIQVLSIDYRLAPEHP
CCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHEEEEECCCCEEEEEEEEEECCCCC
APAAVEDAYAAFVWAHEHASDEFGALPGRVAVGGDSAGGNLSAVVCQLARDKARYEGGPT
CCCHHHHHHEEEEEEECCCCCHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCH
PVLQWLLYPRTDFTAQTRSMGLFGNGFLLTKRDIDWFHTQYLRDSDVDPADPRLSPLLAE
HHHHHHHCCCCCCCHHHHCCCEECCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHH
SLSGLAPALIAVAGFDPLRDEGESYAKALRAAGTAVDLRYLGSLTHGFLNLFQLGGGSAA
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
GTNELISALRAHLSRV
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]