Click here to switch to the map view.

The map label for this gene is rsmD [H]

Identifier: 148824156

GI number: 148824156

Start: 3330374

End: 3330940

Strand: Reverse

Name: rsmD [H]

Synonym: TBFG_12981

Alternate gene names: 148824156

Gene position: 3330940-3330374 (Counterclockwise)

Preceding gene: 148824157

Following gene: 148824155

Centisome position: 75.29

GC content: 69.84

Gene sequence:

>567_bases
TTGACCCGGATCATCGGCGGTGTCGCCGGAGGCCGGCGCATTGCCGTCCCACCACGCGGAACCAGACCTACCACCGATCG
GGTGCGCGAGTCGCTATTCAACATCGTGACTGCGCGGCGGGATCTGACCGGTCTGGCGGTGTTGGACCTCTATGCGGGTT
CCGGCGCCCTGGGGCTGGAGGCGTTGTCGCGGGGAGCGGCGTCCGTGCTGTTCGTGGAGTCCGACCAGCGCAGCGCGGCC
GTCATTGCGCGCAACATCGAGGCCCTAGGTCTCTCCGGTGCGACGCTGCGCCGGGGCGCGGTGGCGGCCGTCGTGGCGGC
CGGGACCACGTCCCCGGTGGATCTGGTGTTGGCCGACCCGCCCTACAACGTCGACTCCGCCGACGTTGACGCCATCCTGG
CCGCACTGGGCACCAACGGCTGGACGCGCGAGGGAACCGTAGCGGTGGTGGAGCGTGCGACCACGTGCGCGCCGTTGACG
TGGCCGGAGGGCTGGCGCCGATGGCCCCAGCGTGTTTACGGCGACACCCGTTTGGAACTGGCCGAACGGCTTTTTGCCAA
CGTGTAG

Upstream 100 bases:

>100_bases
TGAGCACCGCCGGCGACGATGCAGTGGGGGTACCGCCCGCTTGCGGGGGACGAAGCGATGAGGTGGGGGTACCGCCCGCT
TGCGAGGGAGAGCGGCGCAC

Downstream 100 bases:

>100_bases
CGTCATCGGTCATGACCGCCGGCGACGATGCGGTGGGGGCCGTGCCCGCTTGCGGGGGACGCAGCGATGAGGAGGAGCGG
CGCAGATGACGGGCGCGGTA

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTRIIGGVAGGRRIAVPPRGTRPTTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLEALSRGAASVLFVESDQRSAA
VIARNIEALGLSGATLRRGAVAAVVAAGTTSPVDLVLADPPYNVDSADVDAILAALGTNGWTREGTVAVVERATTCAPLT
WPEGWRRWPQRVYGDTRLELAERLFANV

Sequences:

>Translated_188_residues
MTRIIGGVAGGRRIAVPPRGTRPTTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLEALSRGAASVLFVESDQRSAA
VIARNIEALGLSGATLRRGAVAAVVAAGTTSPVDLVLADPPYNVDSADVDAILAALGTNGWTREGTVAVVERATTCAPLT
WPEGWRRWPQRVYGDTRLELAERLFANV
>Mature_187_residues
TRIIGGVAGGRRIAVPPRGTRPTTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLEALSRGAASVLFVESDQRSAAV
IARNIEALGLSGATLRRGAVAAVVAAGTTSPVDLVLADPPYNVDSADVDAILAALGTNGWTREGTVAVVERATTCAPLTW
PEGWRRWPQRVYGDTRLELAERLFANV

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=188, Percent_Identity=36.1702127659575, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19807; Mature: 19676

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIIGGVAGGRRIAVPPRGTRPTTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLE
CCCEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
ALSRGAASVLFVESDQRSAAVIARNIEALGLSGATLRRGAVAAVVAAGTTSPVDLVLADP
HHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCEEEEEECC
PYNVDSADVDAILAALGTNGWTREGTVAVVERATTCAPLTWPEGWRRWPQRVYGDTRLEL
CCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHH
AERLFANV
HHHHHHCC
>Mature Secondary Structure 
TRIIGGVAGGRRIAVPPRGTRPTTDRVRESLFNIVTARRDLTGLAVLDLYAGSGALGLE
CCEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
ALSRGAASVLFVESDQRSAAVIARNIEALGLSGATLRRGAVAAVVAAGTTSPVDLVLADP
HHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCEEEEEECC
PYNVDSADVDAILAALGTNGWTREGTVAVVERATTCAPLTWPEGWRRWPQRVYGDTRLEL
CCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHH
AERLFANV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]