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The map label for this gene is rrmA [C]

Identifier: 148824141

GI number: 148824141

Start: 3315914

End: 3316726

Strand: Direct

Name: rrmA [C]

Synonym: TBFG_12966

Alternate gene names: 148824141

Gene position: 3315914-3316726 (Clockwise)

Preceding gene: 148824133

Following gene: 148824142

Centisome position: 74.95

GC content: 61.99

Gene sequence:

>813_bases
ATGGCCTTCAGTCGAACCCACAGCCTCCTCGCCCGCGCGGGCAGTACCTCGACCTACAAGAGAGTTTGGCGGTACTGGTA
CCCGTTGATGACGCGCGGACTCGGTAACGACGAAATCGTGTTCATCAACTGGGCCTATGAGGAAGATCCGCCGATGGACC
TGCCACTGGAGGCATCCGACGAGCCCAACCGAGCCCACATCAACCTGTACCACCGCACCGCGACCCAGGTCGATCTGGGC
GGCAAGCAGGTGCTGGAGGTCAGTTGCGGACACGGCGGCGGAGCCTCTTACCTCACACGCACGTTGCACCCGGCCTCCTA
CACCGGCCTGGACTTGAACCAGGCGGGAATCAAGTTGTGCAAGAAACGACACCGGCTGCCTGGTTTGGACTTCGTGCGAG
GTGACGCCGAAAACCTGCCCTTCGACGACGAATCCTTCGATGTTGTGCTCAATGTCGAAGCCTCGCACTGTTACCCGCAC
TTTCGGCGTTTCCTCGCCGAGGTGGTTCGCGTGCTGCGCCCAGGAGGGTACTTCCCATACGCCGACCTGCGCCCCAACAA
TGAGATCGCCGCATGGGAGGCCGACCTCGCTGCTACCCCGCTGCGGCAACTGTCGCAGCGGCAAATCAACGCCGAAGTGC
TGCGCGGCATCGGAAACAATTCACAGAAGTCACGGGACCTGGTCGACCGCCATTTGCCGGCCTTCCTGCGTTTCGCGGGC
CGCGAATTCATCGGTGTGCAGGGCACGCAGCTGTCCCGCTACCTGGAAGGCGGGGAACTCTCGTACCGGATGTACTGCTT
CACCAAGGACTGA

Upstream 100 bases:

>100_bases
ACCGGGGTCGGATGTCATGGCATCGACCGCGGGCTCGGTGTTGCGCCAACTTCTCTTCTGACGCATCGCTCGTACATACT
GTCTGCCATACTCCTTGCCC

Downstream 100 bases:

>100_bases
GCCAGTTTCGGGTAATGTCGCCCGGATGAGCCCAGCTGAGCGCGAGTTCGACATCGTTCTATATGGCGCCACCGGCTTCT
CCGGCAAGCTGACCGCCGAA

Product: methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAFSRTHSLLARAGSTSTYKRVWRYWYPLMTRGLGNDEIVFINWAYEEDPPMDLPLEASDEPNRAHINLYHRTATQVDLG
GKQVLEVSCGHGGGASYLTRTLHPASYTGLDLNQAGIKLCKKRHRLPGLDFVRGDAENLPFDDESFDVVLNVEASHCYPH
FRRFLAEVVRVLRPGGYFPYADLRPNNEIAAWEADLAATPLRQLSQRQINAEVLRGIGNNSQKSRDLVDRHLPAFLRFAG
REFIGVQGTQLSRYLEGGELSYRMYCFTKD

Sequences:

>Translated_270_residues
MAFSRTHSLLARAGSTSTYKRVWRYWYPLMTRGLGNDEIVFINWAYEEDPPMDLPLEASDEPNRAHINLYHRTATQVDLG
GKQVLEVSCGHGGGASYLTRTLHPASYTGLDLNQAGIKLCKKRHRLPGLDFVRGDAENLPFDDESFDVVLNVEASHCYPH
FRRFLAEVVRVLRPGGYFPYADLRPNNEIAAWEADLAATPLRQLSQRQINAEVLRGIGNNSQKSRDLVDRHLPAFLRFAG
REFIGVQGTQLSRYLEGGELSYRMYCFTKD
>Mature_269_residues
AFSRTHSLLARAGSTSTYKRVWRYWYPLMTRGLGNDEIVFINWAYEEDPPMDLPLEASDEPNRAHINLYHRTATQVDLGG
KQVLEVSCGHGGGASYLTRTLHPASYTGLDLNQAGIKLCKKRHRLPGLDFVRGDAENLPFDDESFDVVLNVEASHCYPHF
RRFLAEVVRVLRPGGYFPYADLRPNNEIAAWEADLAATPLRQLSQRQINAEVLRGIGNNSQKSRDLVDRHLPAFLRFAGR
EFIGVQGTQLSRYLEGGELSYRMYCFTKD

Specific function: Catalyzes the methylation of the lipid moiety of the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycoserosates to form phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase family

Homologues:

Organism=Caenorhabditis elegans, GI17540496, Length=182, Percent_Identity=32.4175824175824, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHMT_MYCBO (Q7TXK3)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856621.1
- ProteinModelPortal:   Q7TXK3
- SMR:   Q7TXK3
- EnsemblBacteria:   EBMYCT00000014839
- GeneID:   1092129
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2976
- GeneTree:   EBGT00050000014593
- HOGENOM:   HBG567260
- OMA:   RTATHVE
- ProtClustDB:   CLSK791223
- BioCyc:   MBOV233413:MB2976-MONOMER
- InterPro:   IPR013216

Pfam domain/function: PF08241 Methyltransf_11

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30652; Mature: 30521

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSRTHSLLARAGSTSTYKRVWRYWYPLMTRGLGNDEIVFINWAYEEDPPMDLPLEASD
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCC
EPNRAHINLYHRTATQVDLGGKQVLEVSCGHGGGASYLTRTLHPASYTGLDLNQAGIKLC
CCCCEEEEEEEECCCEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHH
KKRHRLPGLDFVRGDAENLPFDDESFDVVLNVEASHCYPHFRRFLAEVVRVLRPGGYFPY
HHHCCCCCCCEECCCCCCCCCCCCCEEEEEEECHHHCCHHHHHHHHHHHHHHCCCCCCCC
ADLRPNNEIAAWEADLAATPLRQLSQRQINAEVLRGIGNNSQKSRDLVDRHLPAFLRFAG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
REFIGVQGTQLSRYLEGGELSYRMYCFTKD
CHHCCCCCHHHHHHHCCCCEEEEEEEEECC
>Mature Secondary Structure 
AFSRTHSLLARAGSTSTYKRVWRYWYPLMTRGLGNDEIVFINWAYEEDPPMDLPLEASD
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCC
EPNRAHINLYHRTATQVDLGGKQVLEVSCGHGGGASYLTRTLHPASYTGLDLNQAGIKLC
CCCCEEEEEEEECCCEEECCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHH
KKRHRLPGLDFVRGDAENLPFDDESFDVVLNVEASHCYPHFRRFLAEVVRVLRPGGYFPY
HHHCCCCCCCEECCCCCCCCCCCCCEEEEEEECHHHCCHHHHHHHHHHHHHHCCCCCCCC
ADLRPNNEIAAWEADLAATPLRQLSQRQINAEVLRGIGNNSQKSRDLVDRHLPAFLRFAG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
REFIGVQGTQLSRYLEGGELSYRMYCFTKD
CHHCCCCCHHHHHHHCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972