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The map label for this gene is istB [H]

Identifier: 148824133

GI number: 148824133

Start: 3301263

End: 3301979

Strand: Direct

Name: istB [H]

Synonym: TBFG_12958

Alternate gene names: 148824133

Gene position: 3301263-3301979 (Clockwise)

Preceding gene: 148824132

Following gene: 148824141

Centisome position: 74.61

GC content: 65.27

Gene sequence:

>717_bases
GTGAGTCAGTGTCCCGGCTGGCCGATCGCGCCCGCGCCGAGAACTGGAGCCACGAAGAATACCTGGCCGCCTGCCTGCAG
CGGGAAGTGTCAGCCCGGGAGTCCCATGGTGGTGAGGGCCGCATCCGCGCCGCCCGCTTCCCGGCTCGGAAGTCGTTGGA
AGAGTTCGACTTTGAGCATGCTCGTGGCCTCAAACGCGACACCATCGCACATCTGGGCACCCTGGATTTCATCACCGCCC
GCGATAACGTCGTGTTTTTGGGCCCCGCCTGGCACCGGGAAGACTCATCTTGCGGTCGGCCTGGCGATACGCGCGTGTCA
GGCCGGTCATCGGGTGCTGTTCGCCACCGCCGCCGAATGGGTAGCACGGCTCGCCGAGGCTCACCACGCCGGGCGCATCT
ACGCCGAACTCACCCGGCTTTGCCGCTATCCGCTCCTGGTGGTTGACGAAGTCGGCTACATTCCGTTTGAGCCCGAGGCC
GCCAACCTCTTCTTCCAGCTGGTGTCCTCCCGGTATGAGCGGGCCAGCTTGATCGTCACGTCCAATAAGGCCTTCGGCCG
GTGGGGCGAGGTTTTCGGCGGCGACGACGTCGTTGCTGCCGCCATGATCGACCGCCTCGTCCACCATGCTGAAGTCGTCG
CCCTCAAAGGCGACAGCTACCGGCTCAAAGACCGCGACCTCGGCCGCGTCCCACCAGCCGGAACCACCGAAGAATAA

Upstream 100 bases:

>100_bases
TGGCGGGGTGGCCTGATGCCCACCACCAAAGCCACCCAGCGCCGTGATGTTTCCACCGAGATCGCTTACCTGACAAGAGC
ATTGAAAGCTCCCACCCTGC

Downstream 100 bases:

>100_bases
CCACCAACCGCCCGGTCTAGGGGGTCAATTTTCAGATGCCGTCAGGGGGTCAGTTTTCGGGTGCCGTTGACACCGTTCAC
AAGGGCGTTTCGAGCAACGC

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSQCPGWPIAPAPRTGATKNTWPPACSGKCQPGSPMVVRAASAPPASRLGSRWKSSTLSMLVASNATPSHIWAPWISSPP
AITSCFWAPPGTGKTHLAVGLAIRACQAGHRVLFATAAEWVARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEA
ANLFFQLVSSRYERASLIVTSNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE

Sequences:

>Translated_238_residues
MSQCPGWPIAPAPRTGATKNTWPPACSGKCQPGSPMVVRAASAPPASRLGSRWKSSTLSMLVASNATPSHIWAPWISSPP
AITSCFWAPPGTGKTHLAVGLAIRACQAGHRVLFATAAEWVARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEA
ANLFFQLVSSRYERASLIVTSNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE
>Mature_237_residues
SQCPGWPIAPAPRTGATKNTWPPACSGKCQPGSPMVVRAASAPPASRLGSRWKSSTLSMLVASNATPSHIWAPWISSPPA
ITSCFWAPPGTGKTHLAVGLAIRACQAGHRVLFATAAEWVARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEAA
NLFFQLVSSRYERASLIVTSNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 25567; Mature: 25436

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQCPGWPIAPAPRTGATKNTWPPACSGKCQPGSPMVVRAASAPPASRLGSRWKSSTLSM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LVASNATPSHIWAPWISSPPAITSCFWAPPGTGKTHLAVGLAIRACQAGHRVLFATAAEW
EEECCCCCCCEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHH
VARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEAANLFFQLVSSRYERASLIVT
HHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
SNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQCPGWPIAPAPRTGATKNTWPPACSGKCQPGSPMVVRAASAPPASRLGSRWKSSTLSM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LVASNATPSHIWAPWISSPPAITSCFWAPPGTGKTHLAVGLAIRACQAGHRVLFATAAEW
EEECCCCCCCEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHH
VARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEAANLFFQLVSSRYERASLIVT
HHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
SNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]