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The map label for this gene is fadD26 [H]

Identifier: 148824139

GI number: 148824139

Start: 3312069

End: 3313928

Strand: Reverse

Name: fadD26 [H]

Synonym: TBFG_12964

Alternate gene names: 148824139

Gene position: 3313928-3312069 (Counterclockwise)

Preceding gene: 148824140

Following gene: 148824138

Centisome position: 74.9

GC content: 60.0

Gene sequence:

>1860_bases
ATGAAAACCAACTCGTCATTTCATGCCGCGGGCGAGGTGGCGACACAACCGGCATGGGGCACCGGTGAGCAAGCGGCACA
ACCGCTGAACGGGAGCACATCGCGATTCGCTATGAGCGAGTCGTCGCTCGCTGATCTCCTACAGAAGGCGGCGAGCCAGT
ACCCCAATCGCGCGGCGTACAAGTTCATTGACTATGACACGGATCCGGCTGGCTTCACCGAAACCGTGACGTGGTGGCAA
GTCCACCGGCGAGCGATGATCGTGGCAGAGGAGCTCTGGATATACGCGTCAAGCGGTGATCGGGTCGCGATATTGGCCCC
GCAGGGCCTTGAATACATCATCGCCTTTATGGGTGTGCTGCAGGCTGGTCTCATTGCGGTTCCTCTTCCGGTGCCACAAT
TCGGCATTCACGACGAACGAATATCTTCGGCATTACGAGACTCTGCGCCGTCCATTATTCTTACGACCTCGTCGGTTATT
GACGAGGTCACGACATATGCGCCACATGCGTGCGCCGCACAGGGCCAGTCCGCGCCCATCGTCGTGGCTGTGGACGCTCT
GGACTTGAGCTCATCCAGAGCGCTCGATCCGACTCGGTTCGAGCGTCCGAGCACAGCATATCTACAGTACACATCCGGTT
CGACCCGCGCGCCGGCCGGTGTTGTCCTCTCGCACAAGAATGTCATCACCAACTGCGTACAGCTAATGTCCGATTACATC
GGGGATTCGGAAAAGGTTCCGTCAACTCCCGTATCGTGGCTGCCGTTCTATCACGACATGGGGTTGATGCTGGGCATCAT
TCTCCCGATGATCAATCAAGACACCGCGGTGTTGATGAGTCCCATGGCATTCTTGCAGCGGCCGGCCCGTTGGATGCAAT
TATTAGCCAAACACCGCGCACAGATTTCCAGCGCACCGAATTTCGGTTTCGAATTGGCGGTACGCAGAACATCCGACGAC
GATATGGCTGGACTTGACCTCGGGCATGTGCGGACGATCGTCACCGGCGCCGAACGAGTAAACGTCGCGACGCTGCGGCG
CTTCACCGAGCGGTTCGCCCCGTTCAACCTCAGCGAGACGGCAATCCGGCCGTCATATGGGCTCGCGGAGGCGACGGTGT
ACGTGGCGACCGCCGGACCGGGACGCGCGCCGAAGAGTGTTTGCTTTGACTACCAGCAGCTGTCGGTCGGTCAGGCGAAG
CGTGCCGAGAACGGCAGCGAGGGAGCCAACCTGGTGAGTTATGGAGCTCCCCGGGCGTCAACCGTGCGGATCGTCGACCC
CGAAACCAGGATGGAGAATCCCGCGGGGACGGTTGGCGAAATCTGGGTGCAGGGAGACAACGTCGGCTTGGGCTACTGGC
GCAACCCGCAGCAGACGGAGGCGACGTTCCGCGCGCGGCTCGTCACTCCCTCGCCCGGGACTTCGGAAGGTCCGTGGCTG
CGGACCGGAGACCTCGGCGTCATTTTCGAGGGCGAACTGTTCATCACGGGTCGCATCAAGGAGCTGTTGGTGGTGGATGG
GGCCAACCACTATCCGGAAGACATCGAGGCGACGATCCAGGAAATCACCGGCGGCCGGGTTGTGGCGATTGCGGTGCCCG
ATGACCGTACTGAAAAGCTGGTAACCATTATCGAACTCATGAAGCGGGGTCGCACAGATGAGGAGGAAAAGAATCGACTC
CGCACCGTCAAACGTGAAGTCGCCTCCGCGATATCCAGGTCACATCGTCTGCGCGTCGCGGATGTCGTTATGGTGGCGCC
GGGTTCTATTCCGGTCACCACCAGCGGTAAGGTTCGGCGTTCGGCCAGCGTTGAGCGTTACCTACATCACGAATTCAGCC
GCCTAGATGCTATGGCATAA

Upstream 100 bases:

>100_bases
CGAGCCCGGGCAAGGTACAGACGCTATGCGCCGACTCGGATTCGTATGAAAGCGGCAGCGCAGCCGTCTGCTCTCTGCCG
TCCATAGCTGAAGGAAGACC

Downstream 100 bases:

>100_bases
CAGCGCATGTATGTGGGGGAAGAATATGACCGAGTGTTTTCTATCTGATCAAGAGATCCGAAAGCTCAATCGTGACCTTC
GAATACTGATAGCAGCTAAT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAYKFIDYDTDPAGFTETVTWWQ
VHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVLQAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVI
DEVTTYAPHACAAQGQSAPIVVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYI
GDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDD
DMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAK
RAENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWL
RTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKLVTIIELMKRGRTDEEEKNRL
RTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVERYLHHEFSRLDAMA

Sequences:

>Translated_619_residues
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAYKFIDYDTDPAGFTETVTWWQ
VHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVLQAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVI
DEVTTYAPHACAAQGQSAPIVVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYI
GDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDD
DMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAK
RAENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWL
RTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKLVTIIELMKRGRTDEEEKNRL
RTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVERYLHHEFSRLDAMA
>Mature_619_residues
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAYKFIDYDTDPAGFTETVTWWQ
VHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVLQAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVI
DEVTTYAPHACAAQGQSAPIVVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYI
GDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDD
DMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAK
RAENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWL
RTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKLVTIIELMKRGRTDEEEKNRL
RTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVERYLHHEFSRLDAMA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=591, Percent_Identity=25.8883248730964, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI225735629, Length=588, Percent_Identity=26.7006802721088, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI45827692, Length=588, Percent_Identity=26.7006802721088, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI55749758, Length=541, Percent_Identity=25.1386321626617, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI225735625, Length=617, Percent_Identity=24.4732576985413, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI45827694, Length=574, Percent_Identity=24.390243902439, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI225735627, Length=518, Percent_Identity=24.7104247104247, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI45827696, Length=518, Percent_Identity=24.7104247104247, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=24.7755834829443, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=668, Percent_Identity=23.2035928143713, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=595, Percent_Identity=22.3529411764706, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=581, Percent_Identity=24.4406196213425, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=540, Percent_Identity=23.5185185185185, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI18859661, Length=535, Percent_Identity=24.2990654205607, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24654656, Length=440, Percent_Identity=22.9545454545455, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581924, Length=544, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67447; Mature: 67447

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAY
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEE
KFIDYDTDPAGFTETVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVL
EEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
QAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVIDEVTTYAPHACAAQGQSAPI
HCCCEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCHHHCCCCCCCE
VVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYI
EEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHC
GDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
QISSAPNFGFELAVRRTSDDDMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSET
HHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHH
AIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAKRAENGSEGANLVSYGAPRAS
CCCCCCCCCEEEEEEEECCCCCCCHHHHHCHHHHCCCHHHHCCCCCCCCCEEECCCCCCC
TVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWL
EEEEECCHHHCCCCCCCCEEEEEECCCEEECCCCCCCHHCCEEEEEEECCCCCCCCCCEE
RTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKL
EECCEEEEEECCEEEEECCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHH
VTIIELMKRGRTDEEEKNRLRTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEECCCCCCHH
SASVERYLHHEFSRLDAMA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAY
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEE
KFIDYDTDPAGFTETVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVL
EEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
QAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVIDEVTTYAPHACAAQGQSAPI
HCCCEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCHHHCCCCCCCE
VVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYI
EEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHC
GDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
QISSAPNFGFELAVRRTSDDDMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSET
HHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHH
AIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAKRAENGSEGANLVSYGAPRAS
CCCCCCCCCEEEEEEEECCCCCCCHHHHHCHHHHCCCHHHHCCCCCCCCCEEECCCCCCC
TVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWL
EEEEECCHHHCCCCCCCCEEEEEECCCEEECCCCCCCHHCCEEEEEEECCCCCCCCCCEE
RTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKL
EECCEEEEEECCEEEEECCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHH
VTIIELMKRGRTDEEEKNRLRTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEECCCCCCHH
SASVERYLHHEFSRLDAMA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]