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The map label for this gene is fadD28 [H]

Identifier: 148824130

GI number: 148824130

Start: 3294808

End: 3296550

Strand: Direct

Name: fadD28 [H]

Synonym: TBFG_12955

Alternate gene names: 148824130

Gene position: 3294808-3296550 (Clockwise)

Preceding gene: 148824128

Following gene: 148824131

Centisome position: 74.47

GC content: 59.44

Gene sequence:

>1743_bases
ATGAGTGTGCGTTCCCTTCCCGCTGCGTTGCGCGCGTGTGCGCGTCTGCAACCCCATGACCCGGCCTTCACGTTTATGGA
TTACGAACAGGACTGGGACGGCGTTGCGATAACCCTGACGTGGTCGCAGCTGTATCGGCGAACGCTGAATGTGGCACAGG
AGCTGAGCCGTTGTGGTTCCACGGGTGACCGCGTGGTGATCTCTGCTCCGCAGGGACTCGAGTACGTCGTCGCCTTTCTC
GGCGCGTTGCAGGCCGGGCGCATCGCCGTGCCGCTTTCGGTTCCACAAGGCGGCGTTACCGATGAACGTTCCGATTCGGT
ACTGAGTGATTCGTCGCCGGTGGCCATTCTCACTACATCGTCTGCCGTGGACGACGTCGTGCAACATGTTGCGCGGCGGC
CCGGGGAATCCCCGCCATCAATTATCGAAGTTGATTTGCTCGATCTGGACGCTCCGAATGGGTATACCTTCAAAGAAGAC
GAGTATCCATCTACCGCGTATTTGCAATACACCTCCGGGTCCACCCGCACGCCCGCTGGCGTGGTGATGTCCCATCAGAA
CGTTCGGGTTAATTTCGAACAGCTGATGTCTGGCTACTTTGCGGATACCGACGGGATTCCACCGCCAAATTCCGCACTCG
TATCCTGGCTACCCTTCTACCACGACATGGGTTTGGTAATAGGAATTTGCGCACCAATTCTGGGTGGATACCCCGCGGTG
CTCACCAGCCCGGTGTCGTTCCTGCAGCGCCCGGCCCGGTGGATGCACTTGATGGCCAGCGATTTTCACGCCTTTTCGGC
AGCACCGAATTTCGCCTTTGAACTAGCGGCACGAAGAACAACCGACGACGACATGGCCGGGCGTGACCTCGGCAACATAC
TGACCATCCTCAGCGGTAGCGAGCGGGTACAGGCCGCGACGATCAAGCGCTTCGCCGACCGCTTTGCTCGCTTCAATCTG
CAGGAGAGGGTGATCCGGCCTTCATACGGGCTCGCAGAAGCAACGGTGTACGTGGCGACGAGCAAACCGGGTCAACCACC
GGAGACCGTCGACTTCGATACTGAAAGTTTATCCGCCGGCCATGCGAAGCCGTGCGCAGGCGGCGGCGCTACATCGTTGA
TCAGCTACATGTTGCCGCGGTCACCGATCGTGCGGATCGTCGACTCGGACACCTGCATCGAATGTCCGGACGGAACCGTC
GGCGAGATCTGGGTGCACGGCGACAACGTCGCTAATGGCTATTGGCAAAAACCCGACGAGAGTGAGCGCACGTTCGGCGG
AAAGATTGTCACCCCTTCGCCGGGCACACCCGAAGGTCCTTGGCTAAGAACGGGCGACTCAGGTTTCGTCACCGATGGCA
AAATGTTCATCATCGGTCGGATCAAAGATCTGCTAATTGTGTACGGACGCAACCACTCCCCCGACGACATCGAGGCAACG
ATCCAGGAGATCACCCGCGGGCGCTGCGCGGCGATCTCGGTTCCCGGTGACCGCAGCACCGAAAAGCTGGTCGCCATTAT
CGAACTCAAGAAGCGTGGCGACTCAGATCAGGACGCGATGGCTAGACTGGGCGCTATTAAACGCGAAGTCACGTCGGCTT
TATCGAGTTCGCACGGTCTCAGCGTCGCGGATCTGGTTCTGGTTGCGCCTGGCTCGATCCCCATTACCACCAGCGGGAAG
GTCAGGAGAGGGGCGTGTGTCGAGCAATATCGACAGGATCAATTCGCCCGCTTGGATGCCTAG

Upstream 100 bases:

>100_bases
TGCTGAGCTGGCCAGATGCGACTTGGCTAAGACCGCGGAGCGCGAGCACACCCCGACGGCGACTGCGACAACTCCAAGCG
TGGCCGGTAACGTGATGCCC

Downstream 100 bases:

>100_bases
TCCGGCTGGCCGTCTACACAGAATTCGGTATATCCGTTTGAAAAAGTCCTCCCCGGACTGCCGCGCCACCATCACCAGCG
GGTCAGCCGACGGTCAGCGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MSVRSLPAALRACARLQPHDPAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFL
GALQAGRIAVPLSVPQGGVTDERSDSVLSDSSPVAILTTSSAVDDVVQHVARRPGESPPSIIEVDLLDLDAPNGYTFKED
EYPSTAYLQYTSGSTRTPAGVVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV
LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDDMAGRDLGNILTILSGSERVQAATIKRFADRFARFNL
QERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAGHAKPCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTV
GEIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFVTDGKMFIIGRIKDLLIVYGRNHSPDDIEAT
IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGK
VRRGACVEQYRQDQFARLDA

Sequences:

>Translated_580_residues
MSVRSLPAALRACARLQPHDPAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFL
GALQAGRIAVPLSVPQGGVTDERSDSVLSDSSPVAILTTSSAVDDVVQHVARRPGESPPSIIEVDLLDLDAPNGYTFKED
EYPSTAYLQYTSGSTRTPAGVVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV
LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDDMAGRDLGNILTILSGSERVQAATIKRFADRFARFNL
QERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAGHAKPCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTV
GEIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFVTDGKMFIIGRIKDLLIVYGRNHSPDDIEAT
IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGK
VRRGACVEQYRQDQFARLDA
>Mature_579_residues
SVRSLPAALRACARLQPHDPAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFLG
ALQAGRIAVPLSVPQGGVTDERSDSVLSDSSPVAILTTSSAVDDVVQHVARRPGESPPSIIEVDLLDLDAPNGYTFKEDE
YPSTAYLQYTSGSTRTPAGVVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAVL
TSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDDMAGRDLGNILTILSGSERVQAATIKRFADRFARFNLQ
ERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAGHAKPCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVG
EIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFVTDGKMFIIGRIKDLLIVYGRNHSPDDIEATI
QEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKV
RRGACVEQYRQDQFARLDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=611, Percent_Identity=24.7135842880524, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI225735629, Length=599, Percent_Identity=24.7078464106845, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI45827692, Length=599, Percent_Identity=24.7078464106845, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI225735625, Length=607, Percent_Identity=24.5469522240527, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI45827694, Length=538, Percent_Identity=24.7211895910781, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI55749758, Length=581, Percent_Identity=24.2685025817556, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI225735627, Length=532, Percent_Identity=24.4360902255639, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI45827696, Length=532, Percent_Identity=24.4360902255639, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI45827698, Length=487, Percent_Identity=23.8193018480493, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788107, Length=570, Percent_Identity=25.9649122807018, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193202679, Length=607, Percent_Identity=24.0527182866557, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=495, Percent_Identity=23.030303030303, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62641; Mature: 62510

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRSLPAALRACARLQPHDPAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGS
CCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
TGDRVVISAPQGLEYVVAFLGALQAGRIAVPLSVPQGGVTDERSDSVLSDSSPVAILTTS
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
SAVDDVVQHVARRPGESPPSIIEVDLLDLDAPNGYTFKEDEYPSTAYLQYTSGSTRTPAG
HHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV
EEEECCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDDMAGRDLGNILTILSGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCHHCCCCHHHHHHHHCCC
ERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAG
CHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHCCCC
HAKPCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNVANGYWQKPDE
CCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCCCC
SERTFGGKIVTPSPGTPEGPWLRTGDSGFVTDGKMFIIGRIKDLLIVYGRNHSPDDIEAT
CCCCCCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEEEECEEEEEECCCCCCHHHHHH
IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTSALSSSHGL
HHHHHCCCEEEEECCCCCCHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SVADLVLVAPGSIPITTSGKVRRGACVEQYRQDQFARLDA
CHHHEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRSLPAALRACARLQPHDPAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGS
CCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
TGDRVVISAPQGLEYVVAFLGALQAGRIAVPLSVPQGGVTDERSDSVLSDSSPVAILTTS
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
SAVDDVVQHVARRPGESPPSIIEVDLLDLDAPNGYTFKEDEYPSTAYLQYTSGSTRTPAG
HHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV
EEEECCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDDMAGRDLGNILTILSGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCHHCCCCHHHHHHHHCCC
ERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAG
CHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHCCCC
HAKPCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNVANGYWQKPDE
CCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCCCC
SERTFGGKIVTPSPGTPEGPWLRTGDSGFVTDGKMFIIGRIKDLLIVYGRNHSPDDIEAT
CCCCCCCEEECCCCCCCCCCEEEECCCCEEECCCEEEEEEECEEEEEECCCCCCHHHHHH
IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTSALSSSHGL
HHHHHCCCEEEEECCCCCCHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
SVADLVLVAPGSIPITTSGKVRRGACVEQYRQDQFARLDA
CHHHEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972; 1527058 [H]