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The map label for this gene is amt

Identifier: 148824109

GI number: 148824109

Start: 3242602

End: 3244035

Strand: Reverse

Name: amt

Synonym: TBFG_12934

Alternate gene names: 148824109

Gene position: 3244035-3242602 (Counterclockwise)

Preceding gene: 148824110

Following gene: 148824108

Centisome position: 73.32

GC content: 63.18

Gene sequence:

>1434_bases
GTGGACCAATTCCCCATCATGGGCGTTCCGGATGGCGGCGACACCGCCTGGATGCTGGTGAGTTCCGCGCTCGTGCTGTT
GATGACGCCGGGTCTGGCATTTTTCTACGGCGGCATGGTGCGTTCCAAGAGCGTCCTGAACATGATCATGATGAGCATCA
GCGCGATGGGTGTCGTGACGGTGCTCTGGGCGCTTTACGGCTACTCGATTGCGTTCGGTGATGACGTTGGCAACATCGCC
GGCAACCCGAGCCAATACTGGGGTCTCAAAGGTCTCATCGGCGTCAATGCTGTTGCTGCCGATCCGAGCACGCAGACCGC
CGCGGTGAATATTCCGCTGGCGGGTACCCTGCCGGCGACCGTGTTTGTGGCATTCCAGCTGATGTTCGCGATCATCACGG
TTGCGCTGATCTCCGGGGCGGTGGCCGACCGGTTGAAGTTCGGCGCCTGGCTGTTGTTCGCCGGTTTGTGGGCGACGTTC
GTCTATTTCCCGGTCGCACACTGGGTCTTCGCATTTGACGGTTTCGCCGCTGAGCACGGCGGCTGGATCGCCAACAAGCT
GCACGCCATCGACTTCGCCGGCGGCACCGCGGTTCATATCAACGCCGGTGTGGCGGCCTTGATGCTGGCGATCGTGCTGG
GCAAAAGACGCGGTTGGCCGGCGACGCTGTTTCGACCGCACAATCTGCCGTTTGTGATGCTCGGGGCCGCCCTGCTGTGG
TTCGGCTGGTACGGGTTCAACGCAGGATCGGCGACGACCGCCAACGGTGTTGCCGGGGCGACGTTTGTCACCACCACGAT
TGCGACGGCCGCGGCCATGCTTGGCTGGCTGCTCACGGAGCGCGTCCGAGATGGCAAGGCTACGACGCTGGGCGCGGCGT
CGGGCATCGTCGCCGGACTGGTCGCCATCACGCCGTCCTGCTCGTCGGTTAATGTCCTGGGTGCCTTGGCGGTAGGCGTG
TCGGCCGGGGTGCTGTGCGCGCTGGCGGTGGGGCTGAAATTCAAACTGGGCTTCGATGATTCGCTCGACGTGGTCGGGGT
GCACCTGGTTGGTGGTCTGGTGGGCACACTGCTGGTGGGTCTGCTGGCAGCTCCGGAGGCTCCGGCAATTAATGGTGTGG
CCGGGGTATCGAAGGGGTTGTTTTACGGCGGAGGCTTCGCCCAGCTGGAACGACAGGCGCTCGGTGCCTGCAGTGTTCTC
GTCTACTCTGGAATCATCACCCTGATCTTGGCATTGATCCTGAAATTCACCATCGGGCTCAGGCTTGACGCGGAGCAAGA
GAGCACCGGCATCGACGAGGCTGAGCACGCGGAAAGCGGCTACGATTTCGCCGTCGCCAGCGGCTCGGTTCTCCCCCCGC
GGGTCACCGTGGAGGATAGCCGCAACGGCATTCAGGAGCGTATAGGCCAGAAAGTGGAGGCGGAGCCGAAATGA

Upstream 100 bases:

>100_bases
GGCGGCACCATCGCCGCAACAACTAGTGCGCATGGTTCTGGATCAGGTCGTCAGTCGTAAGGCTGGGGCCGTGCCAACGC
TCGACTGGAGGTGATAGCGA

Downstream 100 bases:

>100_bases
AGCTGATCACTGCGATCGTGAAGCCGTTCACGCTCGACGACGTCAAGACGAGCCTCGAGGACGCGGGAGTGCTGGGGATG
ACGGTCAGTGAAATCCAGGG

Product: ammonium-transport integral membrane protein amt

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDQFPIMGVPDGGDTAWMLVSSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSISAMGVVTVLWALYGYSIAFGDDVGNIA
GNPSQYWGLKGLIGVNAVAADPSTQTAAVNIPLAGTLPATVFVAFQLMFAIITVALISGAVADRLKFGAWLLFAGLWATF
VYFPVAHWVFAFDGFAAEHGGWIANKLHAIDFAGGTAVHINAGVAALMLAIVLGKRRGWPATLFRPHNLPFVMLGAALLW
FGWYGFNAGSATTANGVAGATFVTTTIATAAAMLGWLLTERVRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALAVGV
SAGVLCALAVGLKFKLGFDDSLDVVGVHLVGGLVGTLLVGLLAAPEAPAINGVAGVSKGLFYGGGFAQLERQALGACSVL
VYSGIITLILALILKFTIGLRLDAEQESTGIDEAEHAESGYDFAVASGSVLPPRVTVEDSRNGIQERIGQKVEAEPK

Sequences:

>Translated_477_residues
MDQFPIMGVPDGGDTAWMLVSSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSISAMGVVTVLWALYGYSIAFGDDVGNIA
GNPSQYWGLKGLIGVNAVAADPSTQTAAVNIPLAGTLPATVFVAFQLMFAIITVALISGAVADRLKFGAWLLFAGLWATF
VYFPVAHWVFAFDGFAAEHGGWIANKLHAIDFAGGTAVHINAGVAALMLAIVLGKRRGWPATLFRPHNLPFVMLGAALLW
FGWYGFNAGSATTANGVAGATFVTTTIATAAAMLGWLLTERVRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALAVGV
SAGVLCALAVGLKFKLGFDDSLDVVGVHLVGGLVGTLLVGLLAAPEAPAINGVAGVSKGLFYGGGFAQLERQALGACSVL
VYSGIITLILALILKFTIGLRLDAEQESTGIDEAEHAESGYDFAVASGSVLPPRVTVEDSRNGIQERIGQKVEAEPK
>Mature_477_residues
MDQFPIMGVPDGGDTAWMLVSSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSISAMGVVTVLWALYGYSIAFGDDVGNIA
GNPSQYWGLKGLIGVNAVAADPSTQTAAVNIPLAGTLPATVFVAFQLMFAIITVALISGAVADRLKFGAWLLFAGLWATF
VYFPVAHWVFAFDGFAAEHGGWIANKLHAIDFAGGTAVHINAGVAALMLAIVLGKRRGWPATLFRPHNLPFVMLGAALLW
FGWYGFNAGSATTANGVAGATFVTTTIATAAAMLGWLLTERVRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALAVGV
SAGVLCALAVGLKFKLGFDDSLDVVGVHLVGGLVGTLLVGLLAAPEAPAINGVAGVSKGLFYGGGFAQLERQALGACSVL
VYSGIITLILALILKFTIGLRLDAEQESTGIDEAEHAESGYDFAVASGSVLPPRVTVEDSRNGIQERIGQKVEAEPK

Specific function: Involved in the uptake of ammonia (Probable)

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Escherichia coli, GI1786656, Length=436, Percent_Identity=43.1192660550459, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17550182, Length=455, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=493, Percent_Identity=27.3833671399594, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=439, Percent_Identity=27.3348519362187, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=468, Percent_Identity=26.4957264957265, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=474, Percent_Identity=34.1772151898734, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=32.7231121281465, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=462, Percent_Identity=29.6536796536797, Blast_Score=220, Evalue=5e-58,
Organism=Drosophila melanogaster, GI161078314, Length=489, Percent_Identity=30.2658486707566, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMT_MYCBO (P63520)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856589.1
- ProteinModelPortal:   P63520
- EnsemblBacteria:   EBMYCT00000016597
- GeneID:   1092162
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2944c
- GeneTree:   EBGT00050000016180
- HOGENOM:   HBG677626
- OMA:   SRVFLHG
- ProtClustDB:   CLSK872123
- BioCyc:   MBOV233413:MB2944C-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 49242; Mature: 49242

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c865e80)-; HASH(0x1cf5ac9c)-; HASH(0x1ce5244c)-; HASH(0x1c4b0af8)-; HASH(0x1cf916b0)-; HASH(0x1cf7a740)-; HASH(0x1ca8b6e4)-; HASH(0x1b8352e4)-; HASH(0x1cea1ca0)-; HASH(0x1cf54cf0)-; HASH(0x1cfbc6d8)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFPIMGVPDGGDTAWMLVSSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSISAMGVVT
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLWALYGYSIAFGDDVGNIAGNPSQYWGLKGLIGVNAVAADPSTQTAAVNIPLAGTLPAT
HHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHH
VFVAFQLMFAIITVALISGAVADRLKFGAWLLFAGLWATFVYFPVAHWVFAFDGFAAEHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
GWIANKLHAIDFAGGTAVHINAGVAALMLAIVLGKRRGWPATLFRPHNLPFVMLGAALLW
CHHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHH
FGWYGFNAGSATTANGVAGATFVTTTIATAAAMLGWLLTERVRDGKATTLGAASGIVAGL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
VAITPSCSSVNVLGALAVGVSAGVLCALAVGLKFKLGFDDSLDVVGVHLVGGLVGTLLVG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLAAPEAPAINGVAGVSKGLFYGGGFAQLERQALGACSVLVYSGIITLILALILKFTIGL
HHCCCCCCCCCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLDAEQESTGIDEAEHAESGYDFAVASGSVLPPRVTVEDSRNGIQERIGQKVEAEPK
EECCCHHCCCCCCHHHHCCCCCEEEECCCCCCCEEEECCCHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MDQFPIMGVPDGGDTAWMLVSSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSISAMGVVT
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLWALYGYSIAFGDDVGNIAGNPSQYWGLKGLIGVNAVAADPSTQTAAVNIPLAGTLPAT
HHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHH
VFVAFQLMFAIITVALISGAVADRLKFGAWLLFAGLWATFVYFPVAHWVFAFDGFAAEHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
GWIANKLHAIDFAGGTAVHINAGVAALMLAIVLGKRRGWPATLFRPHNLPFVMLGAALLW
CHHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHH
FGWYGFNAGSATTANGVAGATFVTTTIATAAAMLGWLLTERVRDGKATTLGAASGIVAGL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
VAITPSCSSVNVLGALAVGVSAGVLCALAVGLKFKLGFDDSLDVVGVHLVGGLVGTLLVG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLAAPEAPAINGVAGVSKGLFYGGGFAQLERQALGACSVLVYSGIITLILALILKFTIGL
HHCCCCCCCCCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLDAEQESTGIDEAEHAESGYDFAVASGSVLPPRVTVEDSRNGIQERIGQKVEAEPK
EECCCHHCCCCCCHHHHCCCCCEEEECCCCCCCEEEECCCHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972