Click here to switch to the map view.

The map label for this gene is glnB

Identifier: 148824108

GI number: 148824108

Start: 3242267

End: 3242605

Strand: Reverse

Name: glnB

Synonym: TBFG_12933

Alternate gene names: 148824108

Gene position: 3242605-3242267 (Counterclockwise)

Preceding gene: 148824109

Following gene: 148824107

Centisome position: 73.29

GC content: 60.77

Gene sequence:

>339_bases
ATGAAGCTGATCACTGCGATCGTGAAGCCGTTCACGCTCGACGACGTCAAGACGAGCCTCGAGGACGCGGGAGTGCTGGG
GATGACGGTCAGTGAAATCCAGGGATACGGACGGCAGAAGGGCCACACGGAGGTTTACCGCGGGGCCGAATACTCGGTGG
ATTTCGTACCGAAGGTTCGGATCGAGGTCGTTGTTGACGATTCCATTGTCGACAAGGTCGTGGACAGCATTGTCCGGGCG
GCGCGCACCGGCAAGATCGGCGACGGCAAGGTGTGGGTGAGTCCGGTAGATACCATCGTGCGGGTGCGCACCGGTGAACG
CGGACACGACGCGTTATGA

Upstream 100 bases:

>100_bases
CCGTCGCCAGCGGCTCGGTTCTCCCCCCGCGGGTCACCGTGGAGGATAGCCGCAACGGCATTCAGGAGCGTATAGGCCAG
AAAGTGGAGGCGGAGCCGAA

Downstream 100 bases:

>100_bases
AACTAGCCGCACTGTGCTGAACCAACCCGGCCGCACCAATTAGCGCTAGCGAGGGAGATGGAGGCAGAAAGTCCTTGTGC
CGCAAGCGATTTAGCTGTCG

Product: nitrogen regulatory protein P-II glnB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRA
ARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL

Sequences:

>Translated_112_residues
MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRA
ARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL
>Mature_112_residues
MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRA
ARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=61.6071428571429, Blast_Score=147, Evalue=2e-37,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=54.4642857142857, Blast_Score=139, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLNB_MYCBO (P64250)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856588.1
- ProteinModelPortal:   P64250
- SMR:   P64250
- EnsemblBacteria:   EBMYCT00000017791
- GeneID:   1092163
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2943c
- GeneTree:   EBGT00050000016728
- HOGENOM:   HBG748089
- OMA:   MDDVREA
- ProtClustDB:   CLSK792161
- BioCyc:   MBOV233413:MB2943C-MONOMER
- GO:   GO:0006350
- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332
- Gene3D:   G3DSA:3.30.70.120
- PRINTS:   PR00340
- SMART:   SM00938

Pfam domain/function: PF00543 P-II; SSF54913 N-reg_PII-like_a/b

EC number: NA

Molecular weight: Translated: 12227; Mature: 12227

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00638 PII_GLNB_CTER; PS51343 PII_GLNB_DOM; PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVR
CCHHHHHHCCCCHHHHHHHHHCCCEECCCHHHHHCCCCCCCCHHHHCCCCCCEECCCCEE
IEVVVDDSIVDKVVDSIVRAARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL
EEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHEEEECCCCCCCCCC
>Mature Secondary Structure
MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVR
CCHHHHHHCCCCHHHHHHHHHCCCEECCCHHHHHCCCCCCCCHHHHCCCCCCEECCCCEE
IEVVVDDSIVDKVVDSIVRAARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL
EEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972