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The map label for this gene is lepB

Identifier: 148824092

GI number: 148824092

Start: 3224499

End: 3225383

Strand: Reverse

Name: lepB

Synonym: TBFG_12917

Alternate gene names: 148824092

Gene position: 3225383-3224499 (Counterclockwise)

Preceding gene: 148824093

Following gene: 148824091

Centisome position: 72.9

GC content: 65.08

Gene sequence:

>885_bases
GTGACCGAAACCACGGACTCCCCATCGGAGCGCCAGCCGGGTCCGGCAGAGCCGGAGCTCTCCTCCCGGGACCCGGACAT
TGCCGGCCAGGTCTTCGACGCAGCCCCGTTCGACGCAGCCCCGGATGCGGACTCCGAAGGCGACTCCAAGGCGGCCAAAA
CGGACGAGCCGCGGCCCGCGAAGCGATCAACGCTGCGGGAGTTCGCGGTGCTGGCGGTGATTGCTGTGGTGCTCTACTAC
GTCATGCTGACGTTTGTCGCGCGCCCTTATCTGATTCCGTCGGAATCGATGGAACCCACGTTGCACGGGTGTTCGACGTG
CGTCGGCGACCGCATCATGGTGGACAAACTCAGCTACCGCTTCGGCTCACCGCAACCTGGCGACGTCATCGTCTTCAGGG
GACCGCCGTCGTGGAACGTTGGTTACAAGTCGATCCGTTCGCACAACGTCGCCGTGCGCTGGGTGCAGAACGCGTTGTCG
TTCATCGGTTTCGTGCCTCCCGACGAGAACGACCTGGTCAAGCGTGTCATCGCGGTCGGCGGACAGACGGTTCAATGCCG
GTCCGACACCGGCCTGACGGTCAACGGCAGGCCACTGAAGGAGCCATACCTGGATCCGGCCACCATGATGGCCGACCCGT
CGATATACCCGTGCCTGGGCAGCGAGTTCGGGCCGGTCACCGTCCCGCCCGGGCGTGTCTGGGTGATGGGCGACAACCGC
ACCCATTCGGCGGATTCCCGCGCTCACTGCCCGTTGCTATGTACTGACGATCCGCTACCGGGGACCGTGCCGGTGGCCAA
CGTCATCGGTAAGGCCAGGTTGATCGTGTGGCCGCCGTCGCGTTGGGGTGTTGTGCGTTCGGTGAATCCCCAGCAAGGTC
GGTAG

Upstream 100 bases:

>100_bases
CGAACTGCGCGGAAAGAAGGCCAAGATCAAGGAGAAGCGCTGACCGGGAACGTCTCGGCGATGTTCGAAAGCCGCCACCT
GCGCTGGCTACGCTGATCTC

Downstream 100 bases:

>100_bases
CCATGGCTGGAATATGACCAAGACCTGGCCACCGCGGACGGTGATCCGTAAATCTGGGGGTTTGCGGGGAATGCGCACCC
TGGAGTCCGCGCTGCATCGT

Product: signal peptidase I lepB (leader peptidase I)

Products: NA

Alternate protein names: SPase I; Leader peptidase I

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADSEGDSKAAKTDEPRPAKRSTLREFAVLAVIAVVLYY
VMLTFVARPYLIPSESMEPTLHGCSTCVGDRIMVDKLSYRFGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALS
FIGFVPPDENDLVKRVIAVGGQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSEFGPVTVPPGRVWVMGDNR
THSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSRWGVVRSVNPQQGR

Sequences:

>Translated_294_residues
MTETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADSEGDSKAAKTDEPRPAKRSTLREFAVLAVIAVVLYY
VMLTFVARPYLIPSESMEPTLHGCSTCVGDRIMVDKLSYRFGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALS
FIGFVPPDENDLVKRVIAVGGQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSEFGPVTVPPGRVWVMGDNR
THSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSRWGVVRSVNPQQGR
>Mature_293_residues
TETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADSEGDSKAAKTDEPRPAKRSTLREFAVLAVIAVVLYYV
MLTFVARPYLIPSESMEPTLHGCSTCVGDRIMVDKLSYRFGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALSF
IGFVPPDENDLVKRVIAVGGQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSEFGPVTVPPGRVWVMGDNRT
HSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSRWGVVRSVNPQQGR

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP_MYCTU (Q10789)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   B70927
- RefSeq:   NP_217419.1
- RefSeq:   NP_337483.1
- ProteinModelPortal:   Q10789
- MEROPS:   S26.024
- EnsemblBacteria:   EBMYCT00000003256
- EnsemblBacteria:   EBMYCT00000068968
- GeneID:   887157
- GeneID:   925307
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2971
- KEGG:   mtu:Rv2903c
- TIGR:   MT2971
- TubercuList:   Rv2903c
- GeneTree:   EBGT00050000017115
- HOGENOM:   HBG596607
- OMA:   RAPPDSE
- ProtClustDB:   CLSK792152
- BRENDA:   3.4.21.89
- GO:   GO:0040007
- GO:   GO:0006508
- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- Gene3D:   G3DSA:2.10.109.10
- PRINTS:   PR00727
- TIGRFAMs:   TIGR02227

Pfam domain/function: PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.89

Molecular weight: Translated: 31880; Mature: 31749

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00501 SPASE_I_1; PS00760 SPASE_I_2; PS00761 SPASE_I_3

Important sites: ACT_SITE 96-96 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

HASH(0x1cfc3bcc)-;

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADSEGDSKAAKTDEPRPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KRSTLREFAVLAVIAVVLYYVMLTFVARPYLIPSESMEPTLHGCSTCVGDRIMVDKLSYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEHHHHHH
FGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALSFIGFVPPDENDLVKRVIAVG
CCCCCCCCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSEFGPVTVPPGRVWVMGDNR
CCEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCC
THSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSRWGVVRSVNPQQGR
CCCCCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCEEEECCCCCCH
>Mature Secondary Structure 
TETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADSEGDSKAAKTDEPRPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KRSTLREFAVLAVIAVVLYYVMLTFVARPYLIPSESMEPTLHGCSTCVGDRIMVDKLSYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEHHHHHH
FGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALSFIGFVPPDENDLVKRVIAVG
CCCCCCCCEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSEFGPVTVPPGRVWVMGDNR
CCEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCC
THSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSRWGVVRSVNPQQGR
CCCCCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCEEEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036