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The map label for this gene is rnhB [H]

Identifier: 148824091

GI number: 148824091

Start: 3223691

End: 3224485

Strand: Reverse

Name: rnhB [H]

Synonym: TBFG_12916

Alternate gene names: 148824091

Gene position: 3224485-3223691 (Counterclockwise)

Preceding gene: 148824092

Following gene: 148824090

Centisome position: 72.88

GC content: 68.18

Gene sequence:

>795_bases
ATGACCAAGACCTGGCCACCGCGGACGGTGATCCGTAAATCTGGGGGTTTGCGGGGAATGCGCACCCTGGAGTCCGCGCT
GCATCGTGGTGGTCTGGGGCCGGTGGCCGGGGTCGACGAAGTGGGTCGCGGCGCCTGCGCTGGCCCGTTGGTGGTCGCGG
CCTGCGTGCTTGGCCCCGGGCGGATAGCAAGTCTTGCCGCCCTTGATGATTCGAAGAAGCTCAGCGAGCAAGCGCGGGAG
AAGCTGTTCCCGCTGATCTGCCGGTATGCGGTGGCCTATCACGTGGTGTTCATCCCGTCGGCCGAGGTCGACCGCCGCGG
CGTGCACGTGGCCAATATCGAAGGTATGCGGCGCGCGGTGGCCGGTCTGGCGGTGCGGCCCGGTTATGTGCTCAGCGACG
GCTTTCGCGTGCCAGGACTGCCGATGCCGTCGTTGCCGGTGATCGGTGGCGACGCGGCAGCCGCCTGCATCGCCGCGGCT
AGCGTGCTCGCCAAGGTCAGCCGGGACCGCGTGATGGTTGCGTTGGACGCCGATCACCCCGGGTACGGTTTCGCGGAGCA
CAAGGGTTACAGCACCCCGGCGCACAGCCGGGCGCTGGCCCGATTGGGACCCTGCCCGCAGCACCGTTACTCGTTCATCA
ATGTTCGCCGGGTGGCGAGTGGGTCGAACACCGCAGAGGTCGCCGACGGCCAACCCGACCCTCGGGACGGGACGGCACAG
ACGGGTGAGGGAAGATGGAGCAAGAGTTCCCACCCAGCGACAATGCGGGCCACGGGTCGCGCTCAGGGCACTTAG

Upstream 100 bases:

>100_bases
AACGTCATCGGTAAGGCCAGGTTGATCGTGTGGCCGCCGTCGCGTTGGGGTGTTGTGCGTTCGGTGAATCCCCAGCAAGG
TCGGTAGCCATGGCTGGAAT

Downstream 100 bases:

>100_bases
GGGCACTGAGCGGGGAAGCCGGGGAACCGGCAGGGAGAAGGACGTCTGAGCAGATGAGTGCAGAGGATCTCGAAAAGTAT
GAAACCGAGATGGAGCTCTC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPGRIASLAALDDSKKLSEQARE
KLFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAVAGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAA
SVLAKVSRDRVMVALDADHPGYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQ
TGEGRWSKSSHPATMRATGRAQGT

Sequences:

>Translated_264_residues
MTKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPGRIASLAALDDSKKLSEQARE
KLFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAVAGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAA
SVLAKVSRDRVMVALDADHPGYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQ
TGEGRWSKSSHPATMRATGRAQGT
>Mature_263_residues
TKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPGRIASLAALDDSKKLSEQAREK
LFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAVAGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAAS
VLAKVSRDRVMVALDADHPGYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQT
GEGRWSKSSHPATMRATGRAQGT

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=183, Percent_Identity=48.6338797814208, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27646; Mature: 27515

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPG
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCC
RIASLAALDDSKKLSEQAREKLFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEECHHHHHHHH
AGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAASVLAKVSRDRVMVALDADHP
HHHHCCCCEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQ
CCCCHHCCCCCCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCC
TGEGRWSKSSHPATMRATGRAQGT
CCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPG
CCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCC
RIASLAALDDSKKLSEQAREKLFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEECHHHHHHHH
AGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAASVLAKVSRDRVMVALDADHP
HHHHCCCCEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQ
CCCCHHCCCCCCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCC
TGEGRWSKSSHPATMRATGRAQGT
CCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]