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The map label for this gene is tsf [H]

Identifier: 148824078

GI number: 148824078

Start: 3209821

End: 3210636

Strand: Reverse

Name: tsf [H]

Synonym: TBFG_12903

Alternate gene names: 148824078

Gene position: 3210636-3209821 (Counterclockwise)

Preceding gene: 148824079

Following gene: 148824077

Centisome position: 72.57

GC content: 67.03

Gene sequence:

>816_bases
ATGGCGAACTTCACTGCCGCTGACGTCAAGCGGCTGAGGGAGCTGACCGGCGCCGGCATGCTCGCCTGTAAGAACGCGCT
GGCCGAAACCGACGGGGACTTCGACAAGGCCGTCGAGGCGCTGCGGATCAAGGGCGCTAAGGATGTCGGCAAGCGTGCCG
AGCGGGCTACGGCCGAGGGCTTGGTGGCGGCCAAGGACGGCGCGCTGATCGAGCTCAACTGCGAGACTGACTTTGTTGCC
AAGAACGCGGAGTTCCAAACGCTGGCCGACCAAGTGGTCGCGGCGGCGGCAGCAGCTAAGCCCGCCGACGTCGACGCGCT
CAAGGGTGCCAGCATCGGCGACAAGACCGTCGAGCAGGCGATCGCCGAGCTGTCGGCCAAGATCGGCGAGAAGCTCGAGC
TGCGTCGTGTGGCGATTTTCGACGGGACCGTGGAAGCCTACCTGCATCGACGTTCCGCTGACCTGCCGCCAGCGGTGGGT
GTACTGGTCGAGTACCGCGGCGACGACGCGGCCGCCGCGCACGCCGTTGCGTTGCAAATCGCCGCGCTGCGGGCGCGGTA
CCTGTCCCGCGACGACGTGCCTGAAGACATCGTGGCCAGCGAACGCCGCATCGCCGAGGAGACGGCAAGGGCCGAGGGCA
AGCCGGAGCAGGCGCTGCCCAAGATTGTCGAGGGCCGGCTGAACGGCTTCTTCAAGGATGCGGTGCTGCTTGAGCAGGCG
TCGGTGTCCGACAATAAGAAGACCGTCAAGGCCCTGCTCGACGTGGCCGGCGTGACGGTGACACGGTTCGTCCGCTTCGA
GGTGGGCCAGGCTTAG

Upstream 100 bases:

>100_bases
CTTCGGCAACGCCATCAGCAACCGCGTCAACAACTGCCCTCACCGACGCCCCCGCAGGCGCAACCGAACCAACCACAGAT
GCATCCTAGGAAAGGCTGAT

Downstream 100 bases:

>100_bases
CCGGGTATGTCGCGCGTACACGCTTTCGTTGACGATGCCCTTGGTGACCTCGACGCGGTCGCCCTCGCCGACGCCATCCG
ATCAGGTCGAGTGGGTCGGG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVA
KNAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQAIAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVG
VLVEYRGDDAAAAHAVALQIAALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQA
SVSDNKKTVKALLDVAGVTVTRFVRFEVGQA

Sequences:

>Translated_271_residues
MANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVA
KNAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQAIAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVG
VLVEYRGDDAAAAHAVALQIAALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQA
SVSDNKKTVKALLDVAGVTVTRFVRFEVGQA
>Mature_270_residues
ANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVAK
NAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQAIAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVGV
LVEYRGDDAAAAHAVALQIAALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQAS
VSDNKKTVKALLDVAGVTVTRFVRFEVGQA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=275, Percent_Identity=39.6363636363636, Blast_Score=174, Evalue=6e-45,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 28755; Mature: 28624

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEG
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
LVAAKDGALIELNCETDFVAKNAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQA
EEEECCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHH
IAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVGVLVEYRGDDAAAAHAVALQI
HHHHHHHHCCHHHHHEEEEECCHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHH
AALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVSDNKKTVKALLDVAGVTVTRFVRFEVGQA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEG
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
LVAAKDGALIELNCETDFVAKNAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQA
EEEECCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHH
IAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVGVLVEYRGDDAAAAHAVALQI
HHHHHHHHCCHHHHHEEEEECCHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHH
AALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVSDNKKTVKALLDVAGVTVTRFVRFEVGQA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA