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The map label for this gene is amiC

Identifier: 148824077

GI number: 148824077

Start: 3208393

End: 3209814

Strand: Reverse

Name: amiC

Synonym: TBFG_12902

Alternate gene names: 148824077

Gene position: 3209814-3208393 (Counterclockwise)

Preceding gene: 148824078

Following gene: 148824076

Centisome position: 72.55

GC content: 66.95

Gene sequence:

>1422_bases
ATGTCGCGCGTACACGCTTTCGTTGACGATGCCCTTGGTGACCTCGACGCGGTCGCCCTCGCCGACGCCATCCGATCAGG
TCGAGTGGGTCGGGCAGACGTCGTCGAGGCCGCCATCGCCCGCGCCGAAGCCGTTAACCCGGCACTGAACGCGTTGGCGT
ATGCGGCTTTCGACGTGGCGCGAGACGCGGCCGCGATGGGTACGGGCCAGGAAGCGTTCTTCAGTGGAGTGCCGACCTTC
ATCAAGGACAACGTCGACGTTGCCGGACAGCCGTCGATGCATGGCACCGACGCGTGGGAACCATACGCGGCCGTCGCCGA
CAGCGAGATAACCCGGGTGGTGCTGGGCACCGGGCTGGTGTCCCTGGGCAAGACGCAGTTGTCGGAATTCGGCTTCAGCG
CCGTGGCCGAACACCCTCGGCTGGGACCGGTCCGTAATCCGTGGAATACCGACTACACAGCGGGTGCCTCCTCATCGGGA
TCGGGCGCCTTGGTGGCAGCCGGCGTGGTGCCGATCGCGCACGCCAACGACGGCGGCGGCTCGATCCGTATTCCGGCCGC
CTGCAACGGGTTGGTCGGGCTCAAGCCGTCGCGCGGCCGGTTGCCGCTGGAGCCGGAGTATCGCAGGTTGCCGGTGGGCA
TCGTCGCCAATGGCGTCCTGACCCGCACGGTACGCGACACCGCAGCCTTCTACCGCGAGGCCGAGCGCCTTTGGCGTAAC
CACCAGCTGCCGCCGGTCGGGGACGTCACAAGCCCCGTTAAGCAGCGGCTGAGGATTGCCGTAGTAACCCGCTCGGTTCT
GCGCGAGGCCAGCCCCGAAGTGCGGCAGCTGACGCTGAAGTTGGCAGGGCTGCTCGAGGAACTAGGTCACCGGGTAGAAC
ACGTCGACCACCCACCGGCACCGGCCAGTTTCGTGGACGATTTCGTGCTCTATTGGGGGTTCTTGGCCCTGGCGCAAGTG
CGCAGCGGCCGGCGCACATTCGGCAGGACGTTCGACCCTACCCGGCTGGACGAACTGACTTTGGGGCTGGCCCGCCATAC
CGGCCGCAACCTGCACCGGCTGCCGTTGGCGATCATGCGTCTGCGCATGCTACGGCGGCGCAGCGTGCGGTTCTTCGGTA
CCTACGACGTCTTGCTCACGCCAACGGTCGCGGAGGCCACTCCGCAGGTCGGATACCTTGCGCCTACCGACTATCAGACG
GTGCTCGACCGGCTGAGCAGCTGGGTCGTGTTCACGCCAGTGCAGAACGTCACGGGGGTGCCGGCGATTTCGCTACCGTT
GGCTCAATCCGCGGACGGCATGCCCGTGGGCATGATGCTCTCGGCTGATACCGGGCGCGAGGCGCTGCTGCTGGAGCTGG
CTTACGAACTCGAAGAGGCCCGGCCCTGGGCCCGGATCCACGCCCCAAATATCGCCGAGTAG

Upstream 100 bases:

>100_bases
GGTGTCCGACAATAAGAAGACCGTCAAGGCCCTGCTCGACGTGGCCGGCGTGACGGTGACACGGTTCGTCCGCTTCGAGG
TGGGCCAGGCTTAGCCGGGT

Downstream 100 bases:

>100_bases
CCCGCGTCCGGATCTAGTCGGACCCGAGCTTCTCCAGCATTCGTTTGAACTCGCGTTGCTGAGGCGCGGTCAGCCTGGCC
AGGACGCGGGCATCGGCGGC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRVHAFVDDALGDLDAVALADAIRSGRVGRADVVEAAIARAEAVNPALNALAYAAFDVARDAAAMGTGQEAFFSGVPTF
IKDNVDVAGQPSMHGTDAWEPYAAVADSEITRVVLGTGLVSLGKTQLSEFGFSAVAEHPRLGPVRNPWNTDYTAGASSSG
SGALVAAGVVPIAHANDGGGSIRIPAACNGLVGLKPSRGRLPLEPEYRRLPVGIVANGVLTRTVRDTAAFYREAERLWRN
HQLPPVGDVTSPVKQRLRIAVVTRSVLREASPEVRQLTLKLAGLLEELGHRVEHVDHPPAPASFVDDFVLYWGFLALAQV
RSGRRTFGRTFDPTRLDELTLGLARHTGRNLHRLPLAIMRLRMLRRRSVRFFGTYDVLLTPTVAEATPQVGYLAPTDYQT
VLDRLSSWVVFTPVQNVTGVPAISLPLAQSADGMPVGMMLSADTGREALLLELAYELEEARPWARIHAPNIAE

Sequences:

>Translated_473_residues
MSRVHAFVDDALGDLDAVALADAIRSGRVGRADVVEAAIARAEAVNPALNALAYAAFDVARDAAAMGTGQEAFFSGVPTF
IKDNVDVAGQPSMHGTDAWEPYAAVADSEITRVVLGTGLVSLGKTQLSEFGFSAVAEHPRLGPVRNPWNTDYTAGASSSG
SGALVAAGVVPIAHANDGGGSIRIPAACNGLVGLKPSRGRLPLEPEYRRLPVGIVANGVLTRTVRDTAAFYREAERLWRN
HQLPPVGDVTSPVKQRLRIAVVTRSVLREASPEVRQLTLKLAGLLEELGHRVEHVDHPPAPASFVDDFVLYWGFLALAQV
RSGRRTFGRTFDPTRLDELTLGLARHTGRNLHRLPLAIMRLRMLRRRSVRFFGTYDVLLTPTVAEATPQVGYLAPTDYQT
VLDRLSSWVVFTPVQNVTGVPAISLPLAQSADGMPVGMMLSADTGREALLLELAYELEEARPWARIHAPNIAE
>Mature_472_residues
SRVHAFVDDALGDLDAVALADAIRSGRVGRADVVEAAIARAEAVNPALNALAYAAFDVARDAAAMGTGQEAFFSGVPTFI
KDNVDVAGQPSMHGTDAWEPYAAVADSEITRVVLGTGLVSLGKTQLSEFGFSAVAEHPRLGPVRNPWNTDYTAGASSSGS
GALVAAGVVPIAHANDGGGSIRIPAACNGLVGLKPSRGRLPLEPEYRRLPVGIVANGVLTRTVRDTAAFYREAERLWRNH
QLPPVGDVTSPVKQRLRIAVVTRSVLREASPEVRQLTLKLAGLLEELGHRVEHVDHPPAPASFVDDFVLYWGFLALAQVR
SGRRTFGRTFDPTRLDELTLGLARHTGRNLHRLPLAIMRLRMLRRRSVRFFGTYDVLLTPTVAEATPQVGYLAPTDYQTV
LDRLSSWVVFTPVQNVTGVPAISLPLAQSADGMPVGMMLSADTGREALLLELAYELEEARPWARIHAPNIAE

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=25.1984126984127, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI195972892, Length=514, Percent_Identity=24.7081712062257, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI166795287, Length=267, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556264, Length=294, Percent_Identity=27.2108843537415, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=304, Percent_Identity=26.9736842105263, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=23.3532934131737, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=30.7291666666667, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=30.7291666666667, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=124, Percent_Identity=37.9032258064516, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=136, Percent_Identity=35.2941176470588, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356731, Length=473, Percent_Identity=24.9471458773784, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652985, Length=510, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=510, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=510, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=510, Percent_Identity=25.2941176470588, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=26.9141531322506, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550774, Length=199, Percent_Identity=29.6482412060301, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=199, Percent_Identity=29.6482412060301, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648437, Length=199, Percent_Identity=29.6482412060301, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24644968, Length=230, Percent_Identity=26.5217391304348, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161078093, Length=473, Percent_Identity=23.6786469344609, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMI3_MYCBO (P63495)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856557.1
- ProteinModelPortal:   P63495
- EnsemblBacteria:   EBMYCT00000017418
- GeneID:   1092195
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2912c
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   NTDHTAG
- ProtClustDB:   PRK07869
- BioCyc:   MBOV233413:MB2912C-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 50917; Mature: 50785

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 82-82 ACT_SITE 157-157 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVHAFVDDALGDLDAVALADAIRSGRVGRADVVEAAIARAEAVNPALNALAYAAFDVA
CCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RDAAAMGTGQEAFFSGVPTFIKDNVDVAGQPSMHGTDAWEPYAAVADSEITRVVLGTGLV
HHHHHCCCCHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
SLGKTQLSEFGFSAVAEHPRLGPVRNPWNTDYTAGASSSGSGALVAAGVVPIAHANDGGG
HHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCC
SIRIPAACNGLVGLKPSRGRLPLEPEYRRLPVGIVANGVLTRTVRDTAAFYREAERLWRN
EEECCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
HQLPPVGDVTSPVKQRLRIAVVTRSVLREASPEVRQLTLKLAGLLEELGHRVEHVDHPPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PASFVDDFVLYWGFLALAQVRSGRRTFGRTFDPTRLDELTLGLARHTGRNLHRLPLAIMR
CHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
LRMLRRRSVRFFGTYDVLLTPTVAEATPQVGYLAPTDYQTVLDRLSSWVVFTPVQNVTGV
HHHHHHCCCEEEEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCC
PAISLPLAQSADGMPVGMMLSADTGREALLLELAYELEEARPWARIHAPNIAE
CEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure 
SRVHAFVDDALGDLDAVALADAIRSGRVGRADVVEAAIARAEAVNPALNALAYAAFDVA
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RDAAAMGTGQEAFFSGVPTFIKDNVDVAGQPSMHGTDAWEPYAAVADSEITRVVLGTGLV
HHHHHCCCCHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
SLGKTQLSEFGFSAVAEHPRLGPVRNPWNTDYTAGASSSGSGALVAAGVVPIAHANDGGG
HHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCC
SIRIPAACNGLVGLKPSRGRLPLEPEYRRLPVGIVANGVLTRTVRDTAAFYREAERLWRN
EEECCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
HQLPPVGDVTSPVKQRLRIAVVTRSVLREASPEVRQLTLKLAGLLEELGHRVEHVDHPPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PASFVDDFVLYWGFLALAQVRSGRRTFGRTFDPTRLDELTLGLARHTGRNLHRLPLAIMR
CHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
LRMLRRRSVRFFGTYDVLLTPTVAEATPQVGYLAPTDYQTVLDRLSSWVVFTPVQNVTGV
HHHHHHCCCEEEEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCC
PAISLPLAQSADGMPVGMMLSADTGREALLLELAYELEEARPWARIHAPNIAE
CEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972