Click here to switch to the map view.

The map label for this gene is hsdM [C]

Identifier: 148823944

GI number: 148823944

Start: 3080416

End: 3082038

Strand: Reverse

Name: hsdM [C]

Synonym: TBFG_12769

Alternate gene names: 148823944

Gene position: 3082038-3080416 (Counterclockwise)

Preceding gene: 148823945

Following gene: 148823943

Centisome position: 69.66

GC content: 61.92

Gene sequence:

>1623_bases
ATGCCGCCCAGGAAGAAGCAGGCGCCGCAGGCGCCGTCGACGATGAAGGAGCTCAAAGACACGCTCTGGAAGGCCGCCGA
CAAGCTGCGCGGGTCGCTGTCGGCCAGCCAATACAAGGACGTGATCCTCGGCCTGGTGTTCCTTAAGTACGTGTCCGACG
CGTATGACGAACGGCGCGAGGCAATCCGTGCCGAGTTGGCGGCCGAAGGAATGGAGGAGTCTCAGATAGAAGACCTGATC
GACGATCCCGAGCAGTACCAGGGTTACGGCGTATTCGTCGTGCCGGTGAGTGCGCGCTGGAAGTTCTTGGCAGAGAACAC
AAAAGGCAAGCCAGCCGTTGGTGGTGAGCCGGCGAAGAACATCGGTCAGCTGATCGACGAGGCGATGGACGCGGTAATGA
AGGCCAATCCAACACTCGGTGGGACGCTGCCGAGGCTGTATAACAAGGACAACATCGACCAGCGCCGGCTCGGTGAGCTG
ATCGACCTATTTAACAGTGCGCGCTTCAGCCGGCAGGGCGAGCACCGCGCCCGGGATCTGATGGGTGAGGTCTACGAATA
CTTCCTCGGCAATTTCGCTCGCGCGGAAGGGAAGCGGGGTGGCGAGTTCTTTACCCCGCCCAGCGTGGTCAAGGTGATCG
TGGAGGTGCTGGAGCCGTCGAGTGGGCGGGTGTATGACCCGTGCTGCGGTTCCGGAGGCATGTTTGTGCAGACCGAGAAG
TTCATCTACGAACACGACGGCGATCCGAAGGATGTCTCGATCTATGGCCAGGAAAGCATTGAGGAGACCTGGCGGATGGC
GAAGATGAACCTCGCCATCCACGGCATCGACAACAAGGGGCTCGGCGCCCGATGGAGTGATACCTTCGCCCGCGACCAGC
ACCCGGACGTGCAGATGGACTACGTGATGGCCAATCTGCCGTTCAACATCAAAGACTGGGCCCGCAACGAGGAAGACCCA
CGCTGGCGCTTCGGTGTTCCGCCCGCCAATAACGCCAACTACGCATGGATTCAGCACATCCTGTACAAGTTGGCGCCGGG
AGGTCGGGCGGGCGTGGTGATGGCCAACGGGTCGATGTCGTCGAACTCCAACGGCGAGGGGGATATTCGCGCGCAGATCG
TGGAGGCGGATTTGGTTTCCTGCATGGTCGCGTTACCCACCCAGCTGTTCCGCAGCACCGGAATCCCGGTGTGCCTGTGG
TTTTTCGCCAAAGACAAGGCGGCAGGTAAGCAAGGGTCTATCGACCGGTGCGGGCAGGTGCTGTTCATCGACGCTCGTGA
ACTGGGCGACCTAGTGGACCGGGCCGAGCGGGCGCTGACCAACGAGGAGATCGTCCGCATCGGGGATACCTTCCACGCGT
GGCGCGGGTCGAAGTCGGCTGCCGTCAAAGGGATTATGTACGAGGATGTTCCGGGGTTCTGTAAGTCGGCGACGTTGGCG
GAGATCAAGGCGACCGACTATGCGCTCACGCCGGGGCGGTATGTGGGTACGCCCGCGGTCGAGGACGACGGAGAGCCGAT
CGACGAGAAGATGGCCCGGTTGTCGAAGGCGTTGCTGGAGGCGTTCGATGAGTCGGCGCGGCTGGAAAGGGTCGTGCGGG
AGCAACTGGGGCGGCTTAGATGA

Upstream 100 bases:

>100_bases
CAGGGACAGCGTGATTCGCCCGATGCGACCTTCGTGTTGTCGAGGAGCTGGCCCACCACGCCCGGCATCGCCGCGGCGCA
TCGATAGGAAGGAAACGGGA

Downstream 100 bases:

>100_bases
GTGATGGATGGAAAACCTTGCGGTTCGGGGAAGTCCTTGAGCTGCAGCGAGGACATGATCTTCCTGCGGCGTCGCGGGGA
TCTGGAACGGTACCTGTTAT

Product: type I restriction/modification system DNA methylase hsdM

Products: NA

Alternate protein names: M.HindVIIP [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERREAIRAELAAEGMEESQIEDLI
DDPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKNIGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGEL
IDLFNSARFSRQGEHRARDLMGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEK
FIYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMDYVMANLPFNIKDWARNEEDP
RWRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMSSNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLW
FFAKDKAAGKQGSIDRCGQVLFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLA
EIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR

Sequences:

>Translated_540_residues
MPPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERREAIRAELAAEGMEESQIEDLI
DDPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKNIGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGEL
IDLFNSARFSRQGEHRARDLMGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEK
FIYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMDYVMANLPFNIKDWARNEEDP
RWRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMSSNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLW
FFAKDKAAGKQGSIDRCGQVLFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLA
EIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR
>Mature_539_residues
PPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERREAIRAELAAEGMEESQIEDLID
DPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKNIGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGELI
DLFNSARFSRQGEHRARDLMGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEKF
IYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMDYVMANLPFNIKDWARNEEDPR
WRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMSSNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLWF
FAKDKAAGKQGSIDRCGQVLFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLAE
IKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=427, Percent_Identity=24.5901639344262, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 60085; Mature: 59954

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERRE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRAELAAEGMEESQIEDLIDDPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKN
HHHHHHHHCCCCHHHHHHHHCCHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHH
IGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGELIDLFNSARFSRQGEHRARDL
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
MGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEHH
FIYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMD
HHEECCCCCCCEEEECHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHCCCCCCCCEEE
YVMANLPFNIKDWARNEEDPRWRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMS
EEEECCCCCHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
SNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLWFFAKDKAAGKQGSIDRCGQV
CCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCHHHCCCE
LFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLA
EEEEHHHHHHHHHHHHHHHCHHHHHEECCHHHHHCCCCHHHHCCCHHHCCCCHHHCCHHH
EIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR
HHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRAELAAEGMEESQIEDLIDDPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKN
HHHHHHHHCCCCHHHHHHHHCCHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHH
IGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGELIDLFNSARFSRQGEHRARDL
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
MGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEHH
FIYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMD
HHEECCCCCCCEEEECHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHCCCCCCCCEEE
YVMANLPFNIKDWARNEEDPRWRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMS
EEEECCCCCHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
SNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLWFFAKDKAAGKQGSIDRCGQV
CCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCHHHCCCE
LFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLA
EEEEHHHHHHHHHHHHHHHCHHHHHEECCHHHHHCCCCHHHHCCCHHHCCCCHHHCCHHH
EIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR
HHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]