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The map label for this gene is miaA

Identifier: 148823915

GI number: 148823915

Start: 3051780

End: 3052724

Strand: Reverse

Name: miaA

Synonym: TBFG_12740

Alternate gene names: 148823915

Gene position: 3052724-3051780 (Counterclockwise)

Preceding gene: 148823916

Following gene: 148823914

Centisome position: 69.0

GC content: 68.68

Gene sequence:

>945_bases
GTGAGACCGTTGGCGATTATCGGACCGACGGGCGCCGGCAAGTCCCAGTTGGCGCTCGACGTCGCCGCGCGGCTCGGCGC
CAGGGTGAGCGTGGAGATCGTGAACGCCGACGCGATGCAGCTGTATCGCGGCATGGACATCGGGACGGCGAAGCTGCCCG
TCAGCGAGCGCCGTGGCATCCCGCATCATCAACTCGATGTCCTGGATGTCACCGAAACCGCGACCGTGGCGCGCTACCAG
CGGGCCGCCGCGGCGGACATCGAGGCGATCGCAGCCCGGGGAGCGGTGCCGGTCGTGGTGGGCGGCTCGATGCTGTATGT
CCAATCCCTGCTCGACGACTGGTCGTTTCCCGCAACCGATCCTTCAGTACGCGCGAGATGGGAGCGGCGTCTGGCCGAGG
TCGGGGTGGATCGGCTGCACGCCGAGTTAGCCCGCCGAGACCCGGCTGCAGCCGCGGCAATCCTGCCCACAGATGCCCGG
CGCACGGTGCGGGCGCTCGAGGTGGTCGAACTCACCGGGCAGCCATTTGCCGCGTCCGCGCCACGCATCGGTGCGCCGCG
GTGGGACACGGTTATCGTCGGGTTGGACTGTCAGACAACGATTCTCGACGAGCGGTTGGCCCGTCGCACCGACCTGATGT
TTGATCAGGGCCTGGTTGAAGAGGTACGCACTCTGCTCCGCAATGGTCTGCGCGAGGGGGTCACCGCGTCACGCGCGCTG
GGCTACGCGCAGGTAATAGCCGCTCTGGACGCCGGTGCTGGAGCTGACATGATGCGCGCCGCGCGGGAGCAGACGTACCT
GGGCACCCGCCGCTACGTGCGACGGCAGCGGTCCTGGTTTCGCCGAGACCACCGGGTGCACTGGCTCGACGCCGGTGTGG
CCAGCTCACCGGACCGCGCACGACTGGTCGACGACGCCGTGCGGTTATGGCGGCACGTAACCTGA

Upstream 100 bases:

>100_bases
AGGTGCTGGCCGGCCTGGCCGAGCCAGGACCGCGATCGGCCAAGGAGTTCTACCGAGGAGCGCCACACGGCGTGGGGTAT
TTCGCCGGTGTCTGGCAGCC

Downstream 100 bases:

>100_bases
TCTGGTGATTGAGGAGTGGCGCAGATGATCTTCGCGAAGGGCCACGGTACCCAGAACGACTTCGTGTTGCTGCCTGACGT
CGACGCCGAGTTGGTGCTCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIGTAKLPVSERRGIPHHQLDVLDVTETATVARYQ
RAAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPATDPSVRARWERRLAEVGVDRLHAELARRDPAAAAAILPTDAR
RTVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCQTTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRAL
GYAQVIAALDAGAGADMMRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDAGVASSPDRARLVDDAVRLWRHVT

Sequences:

>Translated_314_residues
MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIGTAKLPVSERRGIPHHQLDVLDVTETATVARYQ
RAAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPATDPSVRARWERRLAEVGVDRLHAELARRDPAAAAAILPTDAR
RTVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCQTTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRAL
GYAQVIAALDAGAGADMMRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDAGVASSPDRARLVDDAVRLWRHVT
>Mature_314_residues
MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIGTAKLPVSERRGIPHHQLDVLDVTETATVARYQ
RAAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPATDPSVRARWERRLAEVGVDRLHAELARRDPAAAAAILPTDAR
RTVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCQTTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRAL
GYAQVIAALDAGAGADMMRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDAGVASSPDRARLVDDAVRLWRHVT

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=319, Percent_Identity=31.0344827586207, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1790613, Length=307, Percent_Identity=35.1791530944625, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=26.7326732673267, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=28.7671232876712, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=30.4477611940298, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MYCBO (P65353)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856392.1
- ProteinModelPortal:   P65353
- SMR:   P65353
- EnsemblBacteria:   EBMYCT00000015787
- GeneID:   1091816
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2746c
- GeneTree:   EBGT00050000016412
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   MBOV233413:MB2746C-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34447; Mature: 34447

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIGTAKLPVSERRGI
CCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCCCCCHHHHCCC
PHHQLDVLDVTETATVARYQRAAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPATD
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCC
PSVRARWERRLAEVGVDRLHAELARRDPAAAAAILPTDARRTVRALEVVELTGQPFAASA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PRIGAPRWDTVIVGLDCQTTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRAL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GYAQVIAALDAGAGADMMRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDAGVASSPDRA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
RLVDDAVRLWRHVT
HHHHHHHHHHHCCC
>Mature Secondary Structure
MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIGTAKLPVSERRGI
CCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCCCCCHHHHCCC
PHHQLDVLDVTETATVARYQRAAAADIEAIAARGAVPVVVGGSMLYVQSLLDDWSFPATD
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCC
PSVRARWERRLAEVGVDRLHAELARRDPAAAAAILPTDARRTVRALEVVELTGQPFAASA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PRIGAPRWDTVIVGLDCQTTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRAL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GYAQVIAALDAGAGADMMRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDAGVASSPDRA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
RLVDDAVRLWRHVT
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972