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The map label for this gene is dapF

Identifier: 148823914

GI number: 148823914

Start: 3050886

End: 3051755

Strand: Reverse

Name: dapF

Synonym: TBFG_12739

Alternate gene names: 148823914

Gene position: 3051755-3050886 (Counterclockwise)

Preceding gene: 148823915

Following gene: 148823913

Centisome position: 68.98

GC content: 68.16

Gene sequence:

>870_bases
ATGATCTTCGCGAAGGGCCACGGTACCCAGAACGACTTCGTGTTGCTGCCTGACGTCGACGCCGAGTTGGTGCTCACCGC
TGCCCGGGTGGCGGCATTGTGCGACCGGCGGAAGGGGCTGGGCGCCGACGGGGTGTTGCGGGTTACGACCGCCGGAGCCG
CCCAGGCGGTAGGAGTGCTGGACAGCCTGCCCGAGGGTGTCCGCGTGACCGACTGGTACATGGATTACCGCAACGCCGAC
GGGTCGGCAGCCCAGATGTGCGGCAACGGTGTGCGAGTGTTCGCGCACTACTTGCGTGCCAGTGGCCTGGAGGTTCGCGA
CGAGTTCGTCGTAGGGTCACTGGCCGGACCGCGACCAGTCACCTGCCACCACGTCGAGGCGGCCTACGCCGACGTCTCCG
TCGACATGGGCAAGGCCAACAGATTGGGCGCAGGGGAGGCTGTTGTTGGTGGCAGGCGGTTTCACGGCCTGGCCGTCGAT
GTGGGTAACCCGCATCTGGCGTGCGTGGATTCCCAGCTGACCGTCGACGGCCTGGCGGCATTGGACGTGGGTGCGCCGGT
GAGTTTCGATGGTGCGCAGTTCCCCGACGGGGTCAATGTCGAGGTACTCACCGCGCCGGTCGACGGGGCGGTCTGGATGC
GGGTTCACGAACGCGGCGTGGGGGAAACCCGCTCGTGTGGCACCGGGACGGTTGCGGCCGCGGTCGCCGCGTTGGCGGCG
GTGGGCTCGCCGACCGGAACGCTCACCGTGCACGTGCCGGGCGGAGAGGTCGTGGTTACCGTCACCGATGCCACCAGCTT
CCTGCGTGGGCCGTCAGTGTTGGTGGCCCGCGGAGATCTCGCTGACGATTGGTGGAACGCGATGGGGTGA

Upstream 100 bases:

>100_bases
CGCCGGTGTGGCCAGCTCACCGGACCGCGCACGACTGGTCGACGACGCCGTGCGGTTATGGCGGCACGTAACCTGATCTG
GTGATTGAGGAGTGGCGCAG

Downstream 100 bases:

>100_bases
TCCCAGGCAGCGGCTTATGCCGGCAAATTCAGATGCGCGGCCAGCTGCCACGTGCCACCATCGGGTACTGGCTATGACAT
ATCCAGATCCGCCCCAGACG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MIFAKGHGTQNDFVLLPDVDAELVLTAARVAALCDRRKGLGADGVLRVTTAGAAQAVGVLDSLPEGVRVTDWYMDYRNAD
GSAAQMCGNGVRVFAHYLRASGLEVRDEFVVGSLAGPRPVTCHHVEAAYADVSVDMGKANRLGAGEAVVGGRRFHGLAVD
VGNPHLACVDSQLTVDGLAALDVGAPVSFDGAQFPDGVNVEVLTAPVDGAVWMRVHERGVGETRSCGTGTVAAAVAALAA
VGSPTGTLTVHVPGGEVVVTVTDATSFLRGPSVLVARGDLADDWWNAMG

Sequences:

>Translated_289_residues
MIFAKGHGTQNDFVLLPDVDAELVLTAARVAALCDRRKGLGADGVLRVTTAGAAQAVGVLDSLPEGVRVTDWYMDYRNAD
GSAAQMCGNGVRVFAHYLRASGLEVRDEFVVGSLAGPRPVTCHHVEAAYADVSVDMGKANRLGAGEAVVGGRRFHGLAVD
VGNPHLACVDSQLTVDGLAALDVGAPVSFDGAQFPDGVNVEVLTAPVDGAVWMRVHERGVGETRSCGTGTVAAAVAALAA
VGSPTGTLTVHVPGGEVVVTVTDATSFLRGPSVLVARGDLADDWWNAMG
>Mature_289_residues
MIFAKGHGTQNDFVLLPDVDAELVLTAARVAALCDRRKGLGADGVLRVTTAGAAQAVGVLDSLPEGVRVTDWYMDYRNAD
GSAAQMCGNGVRVFAHYLRASGLEVRDEFVVGSLAGPRPVTCHHVEAAYADVSVDMGKANRLGAGEAVVGGRRFHGLAVD
VGNPHLACVDSQLTVDGLAALDVGAPVSFDGAQFPDGVNVEVLTAPVDGAVWMRVHERGVGETRSCGTGTVAAAVAALAA
VGSPTGTLTVHVPGGEVVVTVTDATSFLRGPSVLVARGDLADDWWNAMG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=296, Percent_Identity=30.0675675675676, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_MYCBO (P63898)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856391.1
- ProteinModelPortal:   P63898
- SMR:   P63898
- EnsemblBacteria:   EBMYCT00000015313
- GeneID:   1091823
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2745c
- GeneTree:   EBGT00050000017180
- HOGENOM:   HBG399442
- OMA:   NACGTAM
- ProtClustDB:   PRK00450
- BioCyc:   MBOV233413:MB2745C-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29698; Mature: 29698

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 87-87 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFAKGHGTQNDFVLLPDVDAELVLTAARVAALCDRRKGLGADGVLRVTTAGAAQAVGVL
CEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
DSLPEGVRVTDWYMDYRNADGSAAQMCGNGVRVFAHYLRASGLEVRDEFVVGSLAGPRPV
HHCCCCCEEEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCE
TCHHVEAAYADVSVDMGKANRLGAGEAVVGGRRFHGLAVDVGNPHLACVDSQLTVDGLAA
EEEEEEHEEEEEEEECCCCCCCCCCCEEECCCEEEEEEEECCCCEEEEECCCCEECCEEE
LDVGAPVSFDGAQFPDGVNVEVLTAPVDGAVWMRVHERGVGETRSCGTGTVAAAVAALAA
EECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
VGSPTGTLTVHVPGGEVVVTVTDATSFLRGPSVLVARGDLADDWWNAMG
CCCCCCEEEEEECCCCEEEEEECCHHHHCCCEEEEEECCCCHHHHHHCC
>Mature Secondary Structure
MIFAKGHGTQNDFVLLPDVDAELVLTAARVAALCDRRKGLGADGVLRVTTAGAAQAVGVL
CEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
DSLPEGVRVTDWYMDYRNADGSAAQMCGNGVRVFAHYLRASGLEVRDEFVVGSLAGPRPV
HHCCCCCEEEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCE
TCHHVEAAYADVSVDMGKANRLGAGEAVVGGRRFHGLAVDVGNPHLACVDSQLTVDGLAA
EEEEEEHEEEEEEEECCCCCCCCCCCEEECCCEEEEEEEECCCCEEEEECCCCEECCEEE
LDVGAPVSFDGAQFPDGVNVEVLTAPVDGAVWMRVHERGVGETRSCGTGTVAAAVAALAA
EECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
VGSPTGTLTVHVPGGEVVVTVTDATSFLRGPSVLVARGDLADDWWNAMG
CCCCCCEEEEEECCCCEEEEEECCHHHHCCCEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972