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The map label for this gene is thrS

Identifier: 148823808

GI number: 148823808

Start: 2954506

End: 2956584

Strand: Reverse

Name: thrS

Synonym: TBFG_12633

Alternate gene names: 148823808

Gene position: 2956584-2954506 (Counterclockwise)

Preceding gene: 148823809

Following gene: 148823807

Centisome position: 66.82

GC content: 66.04

Gene sequence:

>2079_bases
ATGAGCGCCCCCGCACAACCCGCCCCGGGAGTCGATGGCGGCGACCCGTCGCAAGCCCGAATTCGGGTTCCTGCCGGGAC
CACCGCGGCCACCGCCGTCGGCGAAGCGGGTTTACCGCGGCGCGGTACGCCCGATGCGATCGTCGTCGTGCGCGACGCCG
ACGGCAACCTGCGCGACCTGAGCTGGGTGCCCGACGTCGACACCGATATCACGCCGGTGGCCGCCAACACCGACGACGGT
CGCAGCGTGATCCGCCATTCGACCGCGCACGTGTTGGCCCAAGCCGTCCAAGAGCTGTTTCCGCAGGCCAAGCTCGGCAT
CGGACCACCCATCACCGACGGCTTCTACTACGACTTCGACGTGCCCGAGCCGTTCACGCCCGAGGACTTGGCGGCGCTGG
AAAAGCGGATGCGCCAGATCGTCAAGGAAGGCCAGCTGTTCGACCGGCGGGTCTACGAATCCACCGAACAGGCCCGCGCC
GAGCTGGCCAACGAGCCCTACAAGCTGGAACTCGTCGACGACAAATCGGGTGACGCCGAGATCATGGAGGTCGGCGGTGA
CGAGCTCACCGCCTACGACAACCTCAACCCCCGCACCCGCGAGCGCGTCTGGGGCGACCTGTGCCGCGGACCGCACATCC
CGACCACCAAACACATCCCGGCGTTCAAGCTCACCCGCAGCTCGGCCGCCTACTGGCGGGGCGATCAGAAAAACGCCAGC
CTGCAACGGATCTACGGCACCGCGTGGGAATCCCAGGAGGCGCTCGACAGGCACCTGGAGTTCATCGAAGAGGCGCAGCG
CCGCGACCACCGCAAGCTGGGTGTCGAGCTGGACCTGTTCAGCTTCCCCGACGAAATCGGTTCCGGCCTAGCGGTTTTCC
ACCCCAAGGGCGGCATCGTGCGTCGCGAACTGGAGGACTACTCGCGGCGCAAGCACACCGAGGCGGGCTACCAGTTCGTC
AACAGCCCGCACATCACCAAGGCCCAGTTGTTCCACACCTCGGGACATCTGGACTGGTACGCCGACGGCATGTTCCCCCC
GATGCACATCGACGCGGAGTACAACGCCGACGGCTCGCTGCGCAAACCCGGCCAGGACTACTACCTCAAGCCGATGAACT
GCCCGATGCACTGCCTGATCTTCCGCGCGCGCGGGCGATCCTATCGGGAACTGCCGTTGCGGCTCTTCGAGTTCGGCACG
GTGTATCGCTACGAGAAGTCCGGTGTGGTGCACGGGTTGACCCGGGTGCGTGGGCTGACCATGGACGACGCGCACATCTT
CTGCACCCGCGACCAGATGCGCGACGAGCTGCGGTCGCTGCTGCGGTTTGTGCTCGACCTGCTCGCCGACTACGGCCTCA
CCGACTTCTACCTCGAACTGTCCACCAAGGACCCGGAGAAGTTCGTCGGCGCCGAGGAGGTCTGGGAGGAAGCCACCACC
GTGCTGGCCGAGGTGGGCGCCGAATCCGGGCTGGAGCTGGTGCCCGATCCAGGCGGCGCGGCGTTCTACGGGCCCAAGAT
TTCAGTGCAGGTCAAAGACGCGCTGGGCCGCACCTGGCAGATGTCGACCATCCAGCTGGACTTCAACTTTCCGGAACGTT
TCGGCCTGGAGTACACCGCCGCCGACGGAACCCGCCACCGCCCGGTGATGATCCACCGCGCGCTATTTGGGTCGATCGAG
CGGTTCTTCGGCATTCTCACCGAGCACTACGCGGGGGCGTTCCCGGCCTGGTTGGCGCCCGTGCAGGTGGTCGGCATCCC
GGTCGCCGATGAGCACGTCGCCTATCTGGAAGAGGTTGCCACGCAACTGAAGTCGCACGGGGTGCGGGCCGAGGTGGACG
CCAGCGACGATCGGATGGCCAAGAAGATCGTGCACCACACCAACCACAAGGTGCCGTTCATGGTGTTGGCGGGTGATCGT
GACGTCGCCGCCGGCGCGGTGAGTTTCCGGTTCGGTGACCGCACCCAAATCAACGGTGTGGCCCGTGACGATGCGGTGGC
GGCCATTGTCGCCTGGATCGCTGACCGCGAAAATGCGGTTCCTACAGCGGAACTGGTGAAAGTGGCCGGTCGTGAGTGA

Upstream 100 bases:

>100_bases
TCGAAAACCCATCCGGTCACTCGGCTTACGGTCGTCGACGTGCGCCCCGATAGCATGGTCGCGATCGTTTCGACACGTCC
AAGCCTTGGAGACCGACCCG

Downstream 100 bases:

>100_bases
CGAGGACCGCACGGATCGGGCCACCGAGGACCACACCATCTTCGATCGGGGTGTCGGCCAGCGCGACCAGCTGCAGCGGT
TATGGACCCCCTACCGGATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDLSWVPDVDTDITPVAANTDDG
RSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFDVPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARA
ELANEPYKLELVDDKSGDAEIMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNAS
LQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFV
NSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGT
VYRYEKSGVVHGLTRVRGLTMDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATT
VLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTAADGTRHRPVMIHRALFGSIE
RFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVATQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDR
DVAAGAVSFRFGDRTQINGVARDDAVAAIVAWIADRENAVPTAELVKVAGRE

Sequences:

>Translated_692_residues
MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDLSWVPDVDTDITPVAANTDDG
RSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFDVPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARA
ELANEPYKLELVDDKSGDAEIMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNAS
LQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFV
NSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGT
VYRYEKSGVVHGLTRVRGLTMDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATT
VLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTAADGTRHRPVMIHRALFGSIE
RFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVATQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDR
DVAAGAVSFRFGDRTQINGVARDDAVAAIVAWIADRENAVPTAELVKVAGRE
>Mature_691_residues
SAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDLSWVPDVDTDITPVAANTDDGR
SVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFDVPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARAE
LANEPYKLELVDDKSGDAEIMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNASL
QRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFVN
SPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTV
YRYEKSGVVHGLTRVRGLTMDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATTV
LAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTAADGTRHRPVMIHRALFGSIER
FFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVATQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRD
VAAGAVSFRFGDRTQINGVARDDAVAAIVAWIADRENAVPTAELVKVAGRE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=616, Percent_Identity=35.8766233766234, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI38202255, Length=616, Percent_Identity=36.525974025974, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI20070344, Length=643, Percent_Identity=35.7698289269051, Blast_Score=347, Evalue=3e-95,
Organism=Escherichia coli, GI1788013, Length=612, Percent_Identity=40.8496732026144, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984184, Length=647, Percent_Identity=34.9304482225657, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71984192, Length=633, Percent_Identity=35.2290679304897, Blast_Score=367, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322112, Length=597, Percent_Identity=38.5259631490787, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322654, Length=350, Percent_Identity=39.7142857142857, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24583842, Length=626, Percent_Identity=37.220447284345, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI28574141, Length=626, Percent_Identity=37.220447284345, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24583839, Length=626, Percent_Identity=37.220447284345, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_MYCBO (P67583)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856292.1
- ProteinModelPortal:   P67583
- SMR:   P67583
- EnsemblBacteria:   EBMYCT00000014473
- GeneID:   1091977
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2646c
- GeneTree:   EBGT00070000032331
- HOGENOM:   HBG352811
- OMA:   NDETMST
- ProtClustDB:   PRK12305
- BioCyc:   MBOV233413:MB2646C-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 77123; Mature: 76991

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDL
CCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEECC
SWVPDVDTDITPVAANTDDGRSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFD
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEECC
VPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARAELANEPYKLELVDDKSGDAE
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
IMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNAS
EEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHCCCCCCCCC
LQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCEEEECCCCCCH
RRELEDYSRRKHTEAGYQFVNSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSL
HHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCEEECCCCCCCCCC
RKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTVYRYEKSGVVHGLTRVRGLT
CCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCC
MDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATT
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHHHHH
VLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTA
HHHHHHHCCCCEECCCCCCCEEECCEEEEEEHHHCCCEEEEEEEEEEECCHHHHCCEEEE
ADGTRHRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHHHHHHHHH
TQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFRFGDRTQINGV
HHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCC
ARDDAVAAIVAWIADRENAVPTAELVKVAGRE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDL
CCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEECC
SWVPDVDTDITPVAANTDDGRSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFD
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEECC
VPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARAELANEPYKLELVDDKSGDAE
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
IMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNAS
EEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHCCCCCCCCC
LQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCEEEECCCCCCH
RRELEDYSRRKHTEAGYQFVNSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSL
HHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCEEECCCCCCCCCC
RKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTVYRYEKSGVVHGLTRVRGLT
CCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCC
MDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATT
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHHHHH
VLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTA
HHHHHHHCCCCEECCCCCCCEEECCEEEEEEHHHCCCEEEEEEEEEEECCHHHHCCEEEE
ADGTRHRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHHHHHHHHH
TQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFRFGDRTQINGV
HHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCC
ARDDAVAAIVAWIADRENAVPTAELVKVAGRE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972