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The map label for this gene is aspS [H]

Identifier: 148823767

GI number: 148823767

Start: 2909330

End: 2911120

Strand: Reverse

Name: aspS [H]

Synonym: TBFG_12592

Alternate gene names: 148823767

Gene position: 2911120-2909330 (Counterclockwise)

Preceding gene: 148823771

Following gene: 148823766

Centisome position: 65.8

GC content: 67.5

Gene sequence:

>1791_bases
GTGTTTGTGCTGCGCAGCCACGCCGCGGGTTTGCTACGGGAAGGCGACGCCGGGCAGCAGGTGACATTGGCCGGCTGGGT
GGCTCGCCGCCGCGACCACGGCGGCGTGATCTTCATCGATCTGCGGGACGCTTCGGGCATCGCACAGGTGGTGTTCCGCG
ACCCCCAGGACACTGAGGTGCTGGCGCAGGCTCACCGGCTGCGTGCGGAATTCTGCGTATCGGTGGCCGGCGTCGTCGAG
ATCCGCCCGGAAGGCAACGCCAACCCGGAGATCGCCACCGGCGAGATCGAGGTCAACGCCACGTCGTTGACCGTGCTGGG
CGAATGTGCGCCGCTGCCGTTTCAGCTGGACGAACCGGCGGGTGAGGAGTTGCGACTGAAGTACCGCTACCTCGATCTGC
GCCGCGACGACCCCGCTGCGGCGATTCGACTGCGTTCCCGGGTGAATGCCGCTGCGCGCGCGGTGCTGGCGCGTCACGAC
TTCGTCGAGATCGAGACGCCGACGATCACCCGCTCGACCCCGGAGGGAGCGCGCGACTTCCTGGTGCCGGCCCGACTGCA
CCCCGGTTCGTTTTACGCCCTACCGCAGAGCCCGCAGCTGTTCAAGCAGCTGCTGATGGTGGCGGGGATGGAACGCTACT
ACCAGATCGCCCGCTGCTACCGCGACGAGGACTTCCGCGCCGACCGCCAGCCCGAATTCACCCAGCTCGATATGGAGATG
AGCTTCGTCGACGCCGAGGACATCATCGCGATCTCCGAGGAAGTTCTGACCGAGCTGTGGGCGCTGATAGGCTACCGGAT
TCCGACGCCCATCCCGCGGATCGGCTATGCCGAAGCGATGCGCCGATTCGGTACGGACAAACCCGACCTGCGGTTCGGGC
TGGAGCTCGTCGAATGCACGGATTTCTTCTCCGACACCACATTTCGTGTCTTCCAGGCACCGTATGTGGGCGCGGTGGTG
ATGCCTGGCGGGGCGTCGCAGCCGCGGCGCACGCTGGACGGCTGGCAGGACTGGGCCAAGCAGCGCGGCCACCGCGGACT
GGCCTACGTGCTGGTCGCCGAAGACGGCACGCTGGGCGGTCCGGTGGCCAAAAACCTGACCGAGGCCGAGCGCACCGGAC
TGGCCGACCATGTCGGGGCCAAACCCGGCGATTGCATCTTCTTCTCGGCCGGTCCGGTCAAATCGTCGCGGGCACTGCTG
GGCGCGGCCCGCGTCGAGATCGCCAACCGGCTGGGCCTGATTGACCCCGATGCGTGGGCATTCGTCTGGGTCGTTGACCC
GCCGCTGTTCGAGCCGGCCGACGAAGCGACCGCCGCCGGTGAGGTCGCGGTCGGCTCGGGGGCCTGGACCGCGGTGCACC
ATGCCTTCACCGCGCCGAAACCGGAATGGGAGGACCGCATCGAATCCGATACCGGCAGCGTGCTGGCCGACGCGTACGAC
ATCGTCTGCAACGGCCACGAGATCGGTGGCGGCTCGGTGCGTATCCACCGCCGTGATATCCAGGAACGGGTGTTCGCGGT
GATGGGCCTGGACAAGGCCGAGGCGGAGGAAAAGTTCGGATTCCTGTTGGAGGCGTTCATGTTTGGCGCACCGCCGCACG
GCGGAATCGCGTTCGGGTGGGACCGGACCACCGCGCTGCTGGCCGGGATGGACTCGATCCGTGAGGTGATCGCGTTCCCG
AAGACCGGCGGCGGCGTCGATCCGCTCACCGACGCGCCGGCGCCCATTACCGCTCAGCAACGTAAGGAGTCCGGAATAGA
TGCCCAGCCCAAGCGGGTCCAGCAGGCATGA

Upstream 100 bases:

>100_bases
GTTGCAATGTTTGTTGAGATGAGCCGAAATTATCCTGCGTGACCGATGTGCGCCGCGGGCACCTCTAGACTGAGCAGTCG
GTTAATCCGGTTGAAGGGGA

Downstream 100 bases:

>100_bases
CCAGATCTTTGCGGTTACGACGTCGCACCCGAGTGTGAATAAATAGGCGTTCATGAGCGCTTCGCTGCTAGTCAGGACGG
CCTGCGGCGGCCGTGCGGTC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEAEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQQA

Sequences:

>Translated_596_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEAEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQQA
>Mature_596_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEAEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQQA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=39.7689768976898, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI45439306, Length=290, Percent_Identity=26.551724137931, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=28.7937743190661, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=28.7937743190661, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4758762, Length=251, Percent_Identity=25.8964143426295, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=581, Percent_Identity=46.815834767642, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=28.2758620689655, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=26.9896193771626, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=610, Percent_Identity=34.0983606557377, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17551876, Length=271, Percent_Identity=27.6752767527675, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=294, Percent_Identity=25.1700680272109, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=266, Percent_Identity=24.0601503759398, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=266, Percent_Identity=24.0601503759398, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=266, Percent_Identity=24.0601503759398, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=639, Percent_Identity=31.924882629108, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=274, Percent_Identity=27.7372262773723, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=38.6776859504132, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24640849, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921528, Length=294, Percent_Identity=25.8503401360544, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136276, Length=238, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65228; Mature: 65228

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEV
CEEECCCCCHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
LAQAHRLRAEFCVSVAGVVEIRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPA
HHHHHHHHHHHHHEEEEEEEECCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEECCCCC
GEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHDFVEIETPTITRSTPEGARDF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHC
LVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
EEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECT
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
DFFSDTTFRVFQAPYVGAVVMPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGG
HHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALLGAARVEIANRLGLIDPDAWA
HHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
EEEEECCCCCCCCCHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHCEE
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEAEEKFGFLLEAFMFGAPPHGGIAFGW
EEECCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECC
DRTTALLAGMDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQQA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEV
CEEECCCCCHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
LAQAHRLRAEFCVSVAGVVEIRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPA
HHHHHHHHHHHHHEEEEEEEECCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEECCCCC
GEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHDFVEIETPTITRSTPEGARDF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHC
LVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
EEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECT
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
DFFSDTTFRVFQAPYVGAVVMPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGG
HHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALLGAARVEIANRLGLIDPDAWA
HHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
EEEEECCCCCCCCCHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHCEE
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEAEEKFGFLLEAFMFGAPPHGGIAFGW
EEECCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECC
DRTTALLAGMDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQQA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]