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The map label for this gene is cobS [H]

Identifier: 148823415

GI number: 148823415

Start: 2484639

End: 2485388

Strand: Direct

Name: cobS [H]

Synonym: TBFG_12236

Alternate gene names: 148823415

Gene position: 2484639-2485388 (Clockwise)

Preceding gene: 148823414

Following gene: 148823416

Centisome position: 56.16

GC content: 71.33

Gene sequence:

>750_bases
GTGATGCGTTCGCTGGCAACAGCTTTCGCATTCGCAACGGTGATACCCACACCGGGCTCAGCGACCACCCCGATGGGCCG
TGGCCCGATGACCGCGCTGCCGGTGGTGGGCGCGGCGCTGGGTGCACTGGCGGCGGCGATCGCATGGGCTGGCGCGCAAG
TGTTCGGCCCGTCCAGCCCGCTGTCCGGCATGCTCACGGTGGCGGTACTGCTGGTCGTCACTCGAGGCCTGCACATCGAT
GGCGTTGCCGATACCGCTGACGGACTGGGCTGCTATGGGCCGCCGCAGCGTGCGCTTGCGGTGATGCGCGACGGGTCGAC
CGGACCGTTCGGGGTGGCGGCCGTGGTCTTGGTCATCGCCTTGCAGGGCCTGGCCTTCGCGACCCTCACCACGGTCGGGA
TCGCTGGGATCACGCTGGCGGTCTTATCCGGCCGGGTCACCGCCGTACTGGTCTGTCGCCGGTTGGTGCCGGCAGCCCAC
GGCAGCACCCTGGGCTCGCGGGTCGCCGGTACGCAACCCGCGCCGGTGGTGGCGGCCTGGCTCGCCGTCCTGCTCGCCGT
TTCGGTGCCGGCCGGTCCCCGGCCTTGGCAAGGACCGATAGCGGTTCTGGTAGCGGTGACGGCCGGCGCGGCCCTGGCGG
CGCATTGCGTGCACCGGTTCGGCGGTGTCACCGGTGACGTGCTGGGCAGCGCGATCGAGCTGAGCACGACGGTCAGCGCC
GTGACGCTTGCGGGCTTGGCCCGGCTTTAG

Upstream 100 bases:

>100_bases
CGGTCGCGGCGCTGTCGTCGATGGCGACCTTCACCGAGGCCGGCGTGTCCACCCGGTCCGTCGACGGTGTCGACCGGACC
GCACCCCCGGCAGTCTCACC

Downstream 100 bases:

>100_bases
CAGGCGGCGAGCGGGACGCTGCAGTAGACTCATGTCCGCCGTCCCTTCCAACACAGGGCTCCCCTCCGTGTCCCCAGATT
AGGGGACATGAAATTCAACC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRLVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL

Sequences:

>Translated_249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRLVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL
>Mature_249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRLVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=250, Percent_Identity=31.2, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 24346; Mature: 24346

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCC
>Mature Secondary Structure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HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]