Click here to switch to the map view.

The map label for this gene is guaB [C]

Identifier: 148823054

GI number: 148823054

Start: 2100455

End: 2101891

Strand: Reverse

Name: guaB [C]

Synonym: TBFG_11871

Alternate gene names: 148823054

Gene position: 2101891-2100455 (Counterclockwise)

Preceding gene: 148823055

Following gene: 148823053

Centisome position: 47.51

GC content: 67.36

Gene sequence:

>1437_bases
ATGAGATTTCTAGACGGGCACCCACCCGGGTACGACCTGACATACAACGACGTGTTCATCGTTCCGAACCGATCCGAGGT
CGCGTCGCGCTTCGACGTCGATTTGTCCACCGCCGACGGCTCGGGCACCACCATTCCGGTAGTGGTCGCCAATATGACCG
CGGTAGCCGGGCGGCGGATGGCCGAGACGGTCGCCCGCCGCGGTGGCATCGTAATCCTGCCGCAGGATCTGCCGATCCCG
GCGGTAAAGCAGACGGTGGCGTTCGTCAAAAGCCGGGACCTGGTGCTCGACACCCCAGTGACGCTGGCACCCGACGATTC
GGTGTCCGACGCCATGGCGCTCATCCACAAGCGCGCACATGGCGTCGCGGTGGTCATCCTCGAGGGTCGCCCGATCGGAT
TGGTGCGCGAATCGTCCTGCCTGGGCGTGGATCGCTTCACCCGGGTGCGCGATATCGCCGTGACGGACTATGTGACCGCT
CCAGCGGGAACCGAGCCACGCAAGATCTTCGACCTGCTGGAGCACGCCCCGGTCGACGTTGCGGTGCTGACCGACGCCGA
CGGCACGTTGGCGGGAGTGCTAAGCCGCACCGGGGCTATCCGCGCCGGTATCTACACCCCGGCCACCGATAGCGCGGGCC
GGCTGCGGATCGGCGCGGCCGTCGGCATCAACGGCGACGTGGGCGCTAAGGCCCGAGCGCTCGCCGAAGCCGGCGTCGAC
GTGCTGGTCATCGACACCGCACACGGACACCAGGTCAAGACCTTGGACGCGATCAAGGCGGTCTCGGCACTGGACTTGGG
TTTGCCGCTGGCGGCGGGAAACGTGGTGTCGGCGGAGGGCACCCGGGATCTGCTGAAAGCCGGGGCGAACGTCGTCAAGG
TCGGTGTCGGCCCCGGCGCCATGTGCACCACCCGAATGATGACCGGTGTCGGCCGCCCGCAGTTCTCCGCTGTGCTCGAA
TGCGCTTCTGCGGCAAGGCAGCTCGGTGGTCATATCTGGGCCGACGGCGGGATCCGGCATCCACGCGACGTGGCTCTGGC
GCTGGCCGCCGGCGCCTCGAACGTGATGATCGGATCGTGGTTCGCCGGCACCTACGAATCCCCCGGAGACCTGATGCGTG
ACCGCGACGATCAGCCGTACAAGGAGAGCTACGGCATGGCATCCAAGCGGGCGGTGGTGGCCCGGACCGGCGCGGACAAC
CCGTTCGACCGAGCTCGTAAGGCGTTGTTCGAGGAAGGCATCTCGACGTCGCGGATGGGCCTTGACCCCGACCGCGGAGG
TGTGGAGGACCTAATTGACCACATCACTTCTGGCGTGCGCAGCACCTGCACCTATGTGGGTGCCTCGAACCTGGCGGAGC
TGCATGAGCGGGCCGTGGTGGGCGTGCAGTCGGGAGCGGGCTTTGCCGAGGGCCATCCGCTGCCCGCCGGCTGGTGA

Upstream 100 bases:

>100_bases
GGATCGACGAACCAGGCAAGTTCCACACACTATGGAGTTCAGACCGCACCGAAGTACCGGTGTAGCGGGCTAGAACTAAA
AGGGGGTAAAGGGGTAAGTG

Downstream 100 bases:

>100_bases
AGCTGGCGCCACGAGCTATCTCGCTACGATGTGTACTTCAAACCCGCAGTATCGCGCTGCGCGGTTGCGAGCGAAAGGGA
TGACGTGCCGCGGGCACCCG

Product: inosine 5-monophosphate dehydrogenase

Products: xanthosine 5'-phosphate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRFLDGHPPGYDLTYNDVFIVPNRSEVASRFDVDLSTADGSGTTIPVVVANMTAVAGRRMAETVARRGGIVILPQDLPIP
AVKQTVAFVKSRDLVLDTPVTLAPDDSVSDAMALIHKRAHGVAVVILEGRPIGLVRESSCLGVDRFTRVRDIAVTDYVTA
PAGTEPRKIFDLLEHAPVDVAVLTDADGTLAGVLSRTGAIRAGIYTPATDSAGRLRIGAAVGINGDVGAKARALAEAGVD
VLVIDTAHGHQVKTLDAIKAVSALDLGLPLAAGNVVSAEGTRDLLKAGANVVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARQLGGHIWADGGIRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRDDQPYKESYGMASKRAVVARTGADN
PFDRARKALFEEGISTSRMGLDPDRGGVEDLIDHITSGVRSTCTYVGASNLAELHERAVVGVQSGAGFAEGHPLPAGW

Sequences:

>Translated_478_residues
MRFLDGHPPGYDLTYNDVFIVPNRSEVASRFDVDLSTADGSGTTIPVVVANMTAVAGRRMAETVARRGGIVILPQDLPIP
AVKQTVAFVKSRDLVLDTPVTLAPDDSVSDAMALIHKRAHGVAVVILEGRPIGLVRESSCLGVDRFTRVRDIAVTDYVTA
PAGTEPRKIFDLLEHAPVDVAVLTDADGTLAGVLSRTGAIRAGIYTPATDSAGRLRIGAAVGINGDVGAKARALAEAGVD
VLVIDTAHGHQVKTLDAIKAVSALDLGLPLAAGNVVSAEGTRDLLKAGANVVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARQLGGHIWADGGIRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRDDQPYKESYGMASKRAVVARTGADN
PFDRARKALFEEGISTSRMGLDPDRGGVEDLIDHITSGVRSTCTYVGASNLAELHERAVVGVQSGAGFAEGHPLPAGW
>Mature_478_residues
MRFLDGHPPGYDLTYNDVFIVPNRSEVASRFDVDLSTADGSGTTIPVVVANMTAVAGRRMAETVARRGGIVILPQDLPIP
AVKQTVAFVKSRDLVLDTPVTLAPDDSVSDAMALIHKRAHGVAVVILEGRPIGLVRESSCLGVDRFTRVRDIAVTDYVTA
PAGTEPRKIFDLLEHAPVDVAVLTDADGTLAGVLSRTGAIRAGIYTPATDSAGRLRIGAAVGINGDVGAKARALAEAGVD
VLVIDTAHGHQVKTLDAIKAVSALDLGLPLAAGNVVSAEGTRDLLKAGANVVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARQLGGHIWADGGIRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRDDQPYKESYGMASKRAVVARTGADN
PFDRARKALFEEGISTSRMGLDPDRGGVEDLIDHITSGVRSTCTYVGASNLAELHERAVVGVQSGAGFAEGHPLPAGW

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI66933016, Length=463, Percent_Identity=30.0215982721382, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI50541954, Length=219, Percent_Identity=42.9223744292237, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI50541952, Length=219, Percent_Identity=42.9223744292237, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI50541948, Length=219, Percent_Identity=42.9223744292237, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI50541956, Length=219, Percent_Identity=42.9223744292237, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI217035148, Length=463, Percent_Identity=29.8056155507559, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI217035146, Length=464, Percent_Identity=29.7413793103448, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI156616279, Length=459, Percent_Identity=30.2832244008715, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI34328928, Length=459, Percent_Identity=30.5010893246187, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI34328930, Length=459, Percent_Identity=30.2832244008715, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI156104880, Length=220, Percent_Identity=41.8181818181818, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI217035152, Length=456, Percent_Identity=29.6052631578947, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI217035150, Length=463, Percent_Identity=28.7257019438445, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1788855, Length=470, Percent_Identity=34.2553191489362, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786293, Length=219, Percent_Identity=41.0958904109589, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560440, Length=220, Percent_Identity=41.3636363636364, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994385, Length=486, Percent_Identity=27.7777777777778, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994389, Length=424, Percent_Identity=28.7735849056604, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322012, Length=470, Percent_Identity=29.3617021276596, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6323464, Length=475, Percent_Identity=28.6315789473684, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323585, Length=471, Percent_Identity=29.2993630573248, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319352, Length=373, Percent_Identity=27.8820375335121, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24641071, Length=466, Percent_Identity=29.6137339055794, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24641073, Length=466, Percent_Identity=29.6137339055794, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI28571163, Length=422, Percent_Identity=29.1469194312796, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): Y1843_MYCTU (P65172)

Other databases:

- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   C70664
- RefSeq:   NP_216359.1
- RefSeq:   NP_336348.1
- ProteinModelPortal:   P65172
- SMR:   P65172
- EnsemblBacteria:   EBMYCT00000000760
- EnsemblBacteria:   EBMYCT00000069718
- GeneID:   885714
- GeneID:   923725
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1891
- KEGG:   mtu:Rv1843c
- TIGR:   MT1891
- TubercuList:   Rv1843c
- GeneTree:   EBGT00050000017422
- HOGENOM:   HBG298985
- OMA:   ESFGMAS
- ProtClustDB:   PRK07807
- GO:   GO:0005829
- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR018529
- InterPro:   IPR001093
- InterPro:   IPR005991
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000130
- SMART:   SM00116
- TIGRFAMs:   TIGR01303

Pfam domain/function: PF00571 CBS; PF00478 IMPDH

EC number: 1.1.1.205

Molecular weight: Translated: 49834; Mature: 49834

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51371 CBS

Important sites: ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLDGHPPGYDLTYNDVFIVPNRSEVASRFDVDLSTADGSGTTIPVVVANMTAVAGRRM
CCCCCCCCCCCCEECCCEEEECCHHHHHHHHCCEEECCCCCCCEEEEEEECHHHHHHHHH
AETVARRGGIVILPQDLPIPAVKQTVAFVKSRDLVLDTPVTLAPDDSVSDAMALIHKRAH
HHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEEECCEEECCCCCHHHHHHHHHHHCC
GVAVVILEGRPIGLVRESSCLGVDRFTRVRDIAVTDYVTAPAGTEPRKIFDLLEHAPVDV
CEEEEEECCCCEEEEECCCCCCHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCCCE
AVLTDADGTLAGVLSRTGAIRAGIYTPATDSAGRLRIGAAVGINGDVGAKARALAEAGVD
EEEECCCCHHHHHHHHCCCEEECEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCC
VLVIDTAHGHQVKTLDAIKAVSALDLGLPLAAGNVVSAEGTRDLLKAGANVVKVGVGPGA
EEEEECCCCCEEHHHHHHHHHHHHHCCCCEECCCEEECCCHHHHHHCCCCEEEEECCCCH
MCTTRMMTGVGRPQFSAVLECASAARQLGGHIWADGGIRHPRDVALALAAGASNVMIGSW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHEEEEECCCCCEEEEEE
FAGTYESPGDLMRDRDDQPYKESYGMASKRAVVARTGADNPFDRARKALFEEGISTSRMG
ECCCCCCCHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
LDPDRGGVEDLIDHITSGVRSTCTYVGASNLAELHERAVVGVQSGAGFAEGHPLPAGW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFLDGHPPGYDLTYNDVFIVPNRSEVASRFDVDLSTADGSGTTIPVVVANMTAVAGRRM
CCCCCCCCCCCCEECCCEEEECCHHHHHHHHCCEEECCCCCCCEEEEEEECHHHHHHHHH
AETVARRGGIVILPQDLPIPAVKQTVAFVKSRDLVLDTPVTLAPDDSVSDAMALIHKRAH
HHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEEECCEEECCCCCHHHHHHHHHHHCC
GVAVVILEGRPIGLVRESSCLGVDRFTRVRDIAVTDYVTAPAGTEPRKIFDLLEHAPVDV
CEEEEEECCCCEEEEECCCCCCHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCCCE
AVLTDADGTLAGVLSRTGAIRAGIYTPATDSAGRLRIGAAVGINGDVGAKARALAEAGVD
EEEECCCCHHHHHHHHCCCEEECEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCC
VLVIDTAHGHQVKTLDAIKAVSALDLGLPLAAGNVVSAEGTRDLLKAGANVVKVGVGPGA
EEEEECCCCCEEHHHHHHHHHHHHHCCCCEECCCEEECCCHHHHHHCCCCEEEEECCCCH
MCTTRMMTGVGRPQFSAVLECASAARQLGGHIWADGGIRHPRDVALALAAGASNVMIGSW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHEEEEECCCCCEEEEEE
FAGTYESPGDLMRDRDDQPYKESYGMASKRAVVARTGADNPFDRARKALFEEGISTSRMG
ECCCCCCCHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
LDPDRGGVEDLIDHITSGVRSTCTYVGASNLAELHERAVVGVQSGAGFAEGHPLPAGW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: inosine 5'-phosphate; NAD+; H2O

Specific reaction: inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036