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The map label for this gene is metK [H]

Identifier: 148822612

GI number: 148822612

Start: 1571166

End: 1572377

Strand: Direct

Name: metK [H]

Synonym: TBFG_11421

Alternate gene names: 148822612

Gene position: 1571166-1572377 (Clockwise)

Preceding gene: 148822611

Following gene: 148822615

Centisome position: 35.51

GC content: 66.67

Gene sequence:

>1212_bases
GTGAGCGAAAAGGGTCGGCTGTTTACCAGTGAGTCGGTGACAGAGGGACATCCCGACAAGATCTGTGACGCCATCAGCGA
CTCGGTTCTGGACGCGCTTCTAGCGGCGGACCCGCGCTCACGTGTCGCGGTCGAGACGCTGGTGACCACCGGGCAGGTGC
ACGTGGTGGGTGAGGTGACCACCTCGGCTAAGGAGGCGTTTGCCGACATCACCAACACGGTCCGCGCACGGATCCTCGAG
ATCGGCTACGACTCGTCGGACAAGGGTTTCGACGGGGCGACCTGCGGGGTGAACATCGGCATCGGCGCACAGTCACCCGA
CATCGCCCAGGGGGTCGACACCGCCCACGAGGCCCGGGTCGAGGGCGCGGCCGATCCGCTGGACTCCCAGGGCGCCGGTG
ACCAGGGCCTGATGTTCGGCTACGCGATCAATGCCACCCCGGAACTGATGCCACTGCCCATCGCGCTGGCCCACCGACTG
TCGCGGCGGCTGACCGAGGTCCGCAAGAACGGGGTGCTGCCCTACCTGCGTCCGGATGGCAAGACGCAGGTCACTATCGC
CTACGAGGACAACGTTCCGGTGCGGCTGGATACCGTGGTCATCTCCACCCAGCACGCGGCCGATATCGACCTGGAGAAGA
CGCTTGATCCCGACATCCGGGAAAAGGTGCTCAACACCGTGCTCGACGACCTGGCCCACGAAACCCTGGACGCGTCGACG
GTGCGGGTGCTGGTGAACCCGACCGGCAAGTTCGTGCTCGGCGGGCCGATGGGCGATGCCGGGCTCACCGGCCGCAAGAT
CATCGTCGACACCTACGGCGGCTGGGCCCGCCACGGCGGCGGCGCCTTCTCCGGCAAGGATCCGTCCAAGGTGGACCGGT
CGGCGGCGTACGCGATGCGCTGGGTGGCCAAGAATGTCGTCGCCGCCGGGTTGGCTGAACGGGTCGAGGTGCAGGTGGCC
TACGCCATCGGTAAAGCGGCACCCGTCGGCCTGTTCGTCGAGACGTTCGGTACCGAGACGGAAGACCCGGTCAAGATCGA
GAAGGCCATCGGCGAGGTATTCGACCTGCGCCCCGGTGCCATCATCCGCGACCTGAACCTGTTGCGCCCGATCTATGCGC
CGACCGCCGCCTACGGGCACTTCGGCCGCACCGACGTCGAATTACCGTGGGAGCAGCTCGACAAGGTCGACGACCTCAAG
CGCGCCATCTAG

Upstream 100 bases:

>100_bases
TCCTGGACAGGTGCTGGACGATCCCTTGCTCGATTGGACGAGCTGAGATTGATGCCTGAGGATATAATTCGGCTAACTAT
TTATCGGAAGGATGACGATA

Downstream 100 bases:

>100_bases
CGTCGAGGGCGCGAGCAGACGCAGAATCGCACGCGGAAAGGCTTCCGCGTGCGATTCTGCGTCTGCTCGGCGCTAGCTGC
TGATGCGGTAGTCGCCGAGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVTTSAKEAFADITNTVRARILE
IGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRL
SRRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERVEVQVA
YAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLK
RAI

Sequences:

>Translated_403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVTTSAKEAFADITNTVRARILE
IGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRL
SRRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERVEVQVA
YAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLK
RAI
>Mature_402_residues
SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVTTSAKEAFADITNTVRARILEI
GYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARVEGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLS
RRLTEVRKNGVLPYLRPDGKTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDASTV
RVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERVEVQVAY
AIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGAIIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKR
AI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=395, Percent_Identity=54.9367088607595, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=53.9845758354756, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=59.4458438287154, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17538494, Length=396, Percent_Identity=54.040404040404, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=54, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17538498, Length=396, Percent_Identity=53.5353535353535, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551082, Length=397, Percent_Identity=52.1410579345088, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=396, Percent_Identity=48.2323232323232, Blast_Score=333, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=349, Percent_Identity=52.1489971346705, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=52.0348837209302, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI32565903, Length=315, Percent_Identity=51.7460317460318, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=92, Percent_Identity=53.2608695652174, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=401, Percent_Identity=54.8628428927681, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=393, Percent_Identity=55.470737913486, Blast_Score=393, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=52.5641025641026, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=51.025641025641, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=51.025641025641, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=51.0582010582011, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=49.5798319327731, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43047; Mature: 42916

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECH
TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHC
EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCC
KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST
CEEEEEEECCCCCEEEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
EEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCH
IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAADPRSRVAVETLVTTGQVHVVGEVT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECH
TSAKEAFADITNTVRARILEIGYDSSDKGFDGATCGVNIGIGAQSPDIAQGVDTAHEARV
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHC
EGAADPLDSQGAGDQGLMFGYAINATPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCC
KTQVTIAYEDNVPVRLDTVVISTQHAADIDLEKTLDPDIREKVLNTVLDDLAHETLDAST
CEEEEEEECCCCCEEEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
EEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNVVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTETEDPVKIEKAIGEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCH
IIRDLNLLRPIYAPTAAYGHFGRTDVELPWEQLDKVDDLKRAI
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA