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The map label for this gene is espA

Identifier: 148663479

GI number: 148663479

Start: 4063644

End: 4064822

Strand: Reverse

Name: espA

Synonym: MRA_3652

Alternate gene names: 148663479

Gene position: 4064822-4063644 (Counterclockwise)

Preceding gene: 148663482

Following gene: 148663478

Centisome position: 91.96

GC content: 63.36

Gene sequence:

>1179_bases
ATGAGCAGAGCGTTCATCATCGATCCAACGATCAGTGCCATTGACGGCTTGTACGACCTTCTGGGGATTGGAATACCCAA
CCAAGGGGGTATCCTTTACTCCTCACTAGAGTACTTCGAAAAAGCCCTGGAGGAGCTGGCAGCAGCGTTTCCGGGTGATG
GCTGGTTAGGTTCGGCCGCGGACAAATACGCCGGCAAAAACCGCAACCACGTGAATTTTTTCCAGGAACTGGCAGACCTC
GATCGTCAGCTCATCAGCCTGATCCACGACCAGGCCAACGCGGTCCAGACGACCCGCGACATCCTGGAGGGCGCCAAGAA
AGGTCTCGAGTTCGTGCGCCCGGTGGCTGTGGACCTGACCTACATCCCGGTCGTCGGGCACGCCCTATCGGCCGCCTTCC
AGGCGCCGTTTTGCGCGGGCGCGATGGCCGTAGTGGGCGGCGCGCTTGCCTACTTGGTCGTGAAAACGCTGATCAACGCG
ACTCAACTCCTCAAATTGCTTGCCAAATTGGCGGAGTTGGTCGCGGCCGCCATTGCGGACATCATTTCGGATGTGGCGGA
CATCATCAAGGGCACCCTCGGAGAAGTGTGGGAGTTCATCACAAACGCGCTCAACGGCCTGAAAGAGCTTTGGGACAAGC
TCACGGGGTGGGTGACCGGACTGTTCTCTCGAGGGTGGTCGAACCTGGAGTCCTTCTTTGCGGGCGTCCCCGGCTTGACC
GGCGCGACCAGCGGCTTGTCGCAAGTGACTGGCTTGTTCGGTGCGGCCGGTCTGTCCGCATCGTCGGGCTTGGCTCACGC
GGATAGCCTGGCGAGCTCAGCCAGCTTGCCCGCCCTGGCCGGCATTGGGGGCGGGTCCGGTTTTGGGGGCTTGCCGAGCC
TGGCTCAGGTCCATGCCGCCTCAACTCGGCAGGCGCTACGGCCCCGAGCTGATGGCCCGGTCGGCGCCGCTGCCGAGCAG
GTCGGCGGGCAGTCGCAGCTGGTCTCCGCGCAGGGTTCCCAAGGTATGGGCGGACCCGTAGGCATGGGCGGCATGCACCC
CTCTTCGGGGGCGTCGAAAGGGACGACGACGAAGAAGTACTCGGAAGGCGCGGCGGCGGGCACTGAAGACGCCGAGCGCG
CGCCAGTCGAAGCTGACGCGGGCGGTGGGCAAAAGGTGCTGGTACGAAACGTCGTCTAA

Upstream 100 bases:

>100_bases
GACATCGGCCAAGCATGACAGCCAGACTGTAGGCCCTACGCGTGCAATGCAGAACCAAGGCTATGCATGGAATCGACGAC
CGTTGAGATAGGCGGCAGGC

Downstream 100 bases:

>100_bases
CGGCATGGCGAGCCAAATCCATTGCTAGCCAGCGCCTAACAACGCGCAATGCTAAACGGAAGGGACACGATCAATGACGG
AAAACTTGACCGTCCAGCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEELAAAFPGDGWLGSAADKYAGKNRNHVNFFQELADL
DRQLISLIHDQANAVQTTRDILEGAKKGLEFVRPVAVDLTYIPVVGHALSAAFQAPFCAGAMAVVGGALAYLVVKTLINA
TQLLKLLAKLAELVAAAIADIISDVADIIKGTLGEVWEFITNALNGLKELWDKLTGWVTGLFSRGWSNLESFFAGVPGLT
GATSGLSQVTGLFGAAGLSASSGLAHADSLASSASLPALAGIGGGSGFGGLPSLAQVHAASTRQALRPRADGPVGAAAEQ
VGGQSQLVSAQGSQGMGGPVGMGGMHPSSGASKGTTTKKYSEGAAAGTEDAERAPVEADAGGGQKVLVRNVV

Sequences:

>Translated_392_residues
MSRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEELAAAFPGDGWLGSAADKYAGKNRNHVNFFQELADL
DRQLISLIHDQANAVQTTRDILEGAKKGLEFVRPVAVDLTYIPVVGHALSAAFQAPFCAGAMAVVGGALAYLVVKTLINA
TQLLKLLAKLAELVAAAIADIISDVADIIKGTLGEVWEFITNALNGLKELWDKLTGWVTGLFSRGWSNLESFFAGVPGLT
GATSGLSQVTGLFGAAGLSASSGLAHADSLASSASLPALAGIGGGSGFGGLPSLAQVHAASTRQALRPRADGPVGAAAEQ
VGGQSQLVSAQGSQGMGGPVGMGGMHPSSGASKGTTTKKYSEGAAAGTEDAERAPVEADAGGGQKVLVRNVV
>Mature_391_residues
SRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEELAAAFPGDGWLGSAADKYAGKNRNHVNFFQELADLD
RQLISLIHDQANAVQTTRDILEGAKKGLEFVRPVAVDLTYIPVVGHALSAAFQAPFCAGAMAVVGGALAYLVVKTLINAT
QLLKLLAKLAELVAAAIADIISDVADIIKGTLGEVWEFITNALNGLKELWDKLTGWVTGLFSRGWSNLESFFAGVPGLTG
ATSGLSQVTGLFGAAGLSASSGLAHADSLASSASLPALAGIGGGSGFGGLPSLAQVHAASTRQALRPRADGPVGAAAEQV
GGQSQLVSAQGSQGMGGPVGMGGMHPSSGASKGTTTKKYSEGAAAGTEDAERAPVEADAGGGQKVLVRNVV

Specific function: Required for virulence. Required for secretion of EsxA (ESAT-6) and EsxB (CFP-10)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Probably secreted via the ESX-1 / type VII secretion system (T7SS)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ESPA_MYCTU (O06267)

Other databases:

- EMBL:   BX842583
- EMBL:   AE000516
- PIR:   A70957
- RefSeq:   NP_218133.1
- RefSeq:   NP_338263.1
- EnsemblBacteria:   EBMYCT00000000738
- EnsemblBacteria:   EBMYCT00000069339
- GeneID:   885377
- GeneID:   922775
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3718
- KEGG:   mtu:Rv3616c
- TIGR:   MT3718
- TubercuList:   Rv3616c
- GeneTree:   EBGT00050000014868
- HOGENOM:   HBG569597
- OMA:   SAINGLH
- ProtClustDB:   CLSK792598
- GO:   GO:0009405

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39889; Mature: 39757

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCHHHCCCCCCCCCCCEEHHCCCC
>Mature Secondary Structure 
SRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEELAAAFPGDGWLGSAA
CCCEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH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CCCCCCCCCCHHHCCCCCCCCCCCEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036