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The map label for this gene is hsaA

Identifier: 148663434

GI number: 148663434

Start: 4019752

End: 4020936

Strand: Reverse

Name: hsaA

Synonym: MRA_3609

Alternate gene names: 148663434

Gene position: 4020936-4019752 (Counterclockwise)

Preceding gene: 148663437

Following gene: 148663433

Centisome position: 90.97

GC content: 65.4

Gene sequence:

>1185_bases
GTGACGTCCATTCAACAGCGTGATGCTCAGTCTGTCTTGGCTGCCATCGATAATCTGCTGCCGGAGATTCGGGATCGCGC
CCAGGCCACGGAGGATCTGCGGCGGCTGCCCGACGAGACCGTCAAGGCGCTCGACGACGTCGGCTTCTTCACCCTGTTGC
AGCCGCAGCAGTGGGGCGGACTGCAGTGCGACCCTGCGCTGTTCTTCGAGGCGACGCGTCGACTGGCGAGCGTGTGTGGT
TCCACCGGGTGGGTGAGCTCAATCGTCGGCGTGCACAACTGGCATCTGGCGCTGTTCGACCAACGGGCCCAAGAGGAGGT
CTGGGGCGAGGACCCGAGTACGCGGATCTCGTCCTCGTACGCACCGATGGGGGCGGGCGTGGTGGTCGACGGTGGCTACC
TGGTCAACGGGTCGTGGAACTGGTCCTCGGGCTGCGACCATGCCAGCTGGACGTTCGTCGGCGGCCCGGTCATCAAGGAC
GGCCGGCCGGTGGACTTCGGCAGTTTCCTGATCCCGCGCAGCGAATACGAGATCAAGGACGTGTGGTACGTGGTCGGCTT
ACGTGGTACCGGCAGCAACACCCTGGTGGTCAAGGATGTCTTTGTGCCCCGGCACCGGTTCTTGTCGTACAAGGCGATGA
ACGACCACACCGCGGGAGGGCTGGCGACCAACAGCGCACCTGTCTATAAAATGCCATGGGGCACAATGCATCCCACTACG
ATTTCGGCGCCGATTGTCGGCATGGCCTACGGTGCCTACGCCGCGCACGTCGAGCACCAGGGCAAGCGGGTGCGCGCGGC
GTTCGCCGGGGAAAAGGCCAAAGACGACCCGTTTGCCAAGGTCCGTATCGCCGAGGCGGCCAGCGACATCGACGCCGCGT
GGCGCCAACTGATCGGTAATGTGAGCGACGAATACGCGCTGCTGGCTGCCGGCAAGGAGATTCCGTTCGAACTGCGCGCC
CGTGCGCGCCGCGACCAGGTGCGCGCCACCGGTCGCTCAATCGCCTCGATCGACCGGCTGTTCGAGGCGTCCGGTGCCAC
CGCGTTGTCCAATGAGGCTCCGATTCAACGGTTCTGGCGCGACGCGCACGCCGGTCGGGTGCACGCCGCCAACGACCCCG
AGCGGGCATACGTGATCTTTGGAAACCACGAGTTCGGGTTGCCGCCCGGCGACACCATGGTCTAG

Upstream 100 bases:

>100_bases
CATTTGGTATCGCTACCTGCCGCAGGAATACCTAGCTCGACACAAATCGGAACGTGTTCTAGTCTTTCTCTAAGTTCGCA
ACCCGGAGAGGCAAAAGTAA

Downstream 100 bases:

>100_bases
CTGGTCTAGGCGAAATGACAGCTACCGAGGAATTGACGTTCGAATCCACCTCGCGCTTTGCGGAAGTGGACGTCGACGGG
CCGCTGAAACTGCACTACCA

Product: putative hydroxylase

Products: NA

Alternate protein names: 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTSIQQRDAQSVLAAIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGGLQCDPALFFEATRRLASVCG
STGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSYAPMGAGVVVDGGYLVNGSWNWSSGCDHASWTFVGGPVIKD
GRPVDFGSFLIPRSEYEIKDVWYVVGLRGTGSNTLVVKDVFVPRHRFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTT
ISAPIVGMAYGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLAAGKEIPFELRA
RARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWRDAHAGRVHAANDPERAYVIFGNHEFGLPPGDTMV

Sequences:

>Translated_394_residues
MTSIQQRDAQSVLAAIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGGLQCDPALFFEATRRLASVCG
STGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSYAPMGAGVVVDGGYLVNGSWNWSSGCDHASWTFVGGPVIKD
GRPVDFGSFLIPRSEYEIKDVWYVVGLRGTGSNTLVVKDVFVPRHRFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTT
ISAPIVGMAYGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLAAGKEIPFELRA
RARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWRDAHAGRVHAANDPERAYVIFGNHEFGLPPGDTMV
>Mature_393_residues
TSIQQRDAQSVLAAIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGGLQCDPALFFEATRRLASVCGS
TGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSYAPMGAGVVVDGGYLVNGSWNWSSGCDHASWTFVGGPVIKDG
RPVDFGSFLIPRSEYEIKDVWYVVGLRGTGSNTLVVKDVFVPRHRFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTTI
SAPIVGMAYGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLAAGKEIPFELRAR
ARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWRDAHAGRVHAANDPERAYVIFGNHEFGLPPGDTMV

Specific function: Catalyzes the o-hydroxylation of 3-hydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy- 9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Also catalyzes the o-hydroxylation of a range of

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSAA_MYCTU (P96852)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842583
- PIR:   H70605
- RefSeq:   NP_218087.1
- RefSeq:   NP_338220.1
- PDB:   3AFE
- PDB:   3AFF
- PDBsum:   3AFE
- PDBsum:   3AFF
- ProteinModelPortal:   P96852
- SMR:   P96852
- EnsemblBacteria:   EBMYCT00000003333
- EnsemblBacteria:   EBMYCT00000072069
- GeneID:   887241
- GeneID:   922818
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3675
- KEGG:   mtu:Rv3570c
- TIGR:   MT3675
- TubercuList:   Rv3570c
- GeneTree:   EBGT00050000015296
- HOGENOM:   HBG699734
- OMA:   PEQWGGL
- ProtClustDB:   CLSK872206
- InterPro:   IPR013107
- InterPro:   IPR006092
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100
- Gene3D:   G3DSA:2.40.110.10
- Gene3D:   G3DSA:1.10.540.10
- Gene3D:   G3DSA:1.20.140.10

Pfam domain/function: PF08028 Acyl-CoA_dh_2; PF02771 Acyl-CoA_dh_N; SSF56645 AcylCoA_dehyd_NM; SSF47203 AcylCoADH_C_like

EC number: NA

Molecular weight: Translated: 43143; Mature: 43012

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQQRDAQSVLAAIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCC
LQCDPALFFEATRRLASVCGSTGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHCCCC
APMGAGVVVDGGYLVNGSWNWSSGCDHASWTFVGGPVIKDGRPVDFGSFLIPRSEYEIKD
CCCCCCEEEECCEEEECCCCCCCCCCCCCEEEECCCEECCCCCCCCCCEECCCCCCCEEE
VWYVVGLRGTGSNTLVVKDVFVPRHRFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTT
EEEEEEEECCCCCEEEEEEECCCHHHHEEEEECCCCCCCCEECCCCCEEECCCCCCCCCC
ISAPIVGMAYGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGN
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCC
VSDEYALLAAGKEIPFELRARARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWR
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DAHAGRVHAANDPERAYVIFGNHEFGLPPGDTMV
HHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TSIQQRDAQSVLAAIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCC
LQCDPALFFEATRRLASVCGSTGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHCCCC
APMGAGVVVDGGYLVNGSWNWSSGCDHASWTFVGGPVIKDGRPVDFGSFLIPRSEYEIKD
CCCCCCEEEECCEEEECCCCCCCCCCCCCEEEECCCEECCCCCCCCCCEECCCCCCCEEE
VWYVVGLRGTGSNTLVVKDVFVPRHRFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTT
EEEEEEEECCCCCEEEEEEECCCHHHHEEEEECCCCCCCCEECCCCCEEECCCCCCCCCC
ISAPIVGMAYGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGN
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCC
VSDEYALLAAGKEIPFELRARARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWR
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DAHAGRVHAANDPERAYVIFGNHEFGLPPGDTMV
HHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036