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The map label for this gene is mhpD [H]

Identifier: 148663399

GI number: 148663399

Start: 3983177

End: 3983962

Strand: Reverse

Name: mhpD [H]

Synonym: MRA_3575

Alternate gene names: 148663399

Gene position: 3983962-3983177 (Counterclockwise)

Preceding gene: 148663403

Following gene: 148663398

Centisome position: 90.14

GC content: 66.41

Gene sequence:

>786_bases
ATGCTCCGTGATGCCACCCGTGATGAGCTGGCCGCCGACCTAGCACAAGCCGAGCGAAGCCGCGACCCGATCGGTCAACT
GACCGCCGCCCATCCCGAGATCGATGTCGTCGACGCCTACGAGATCCAGTTGATCAACATCCGGCAGCGGGTGGCCGAGG
GTGCCCGCGTGGTGGGTCATAAGGTCGGGCTGTCGTCGCCGATCATGCAGCAGATGATGGGGGTTGACGAACCAGACTAC
GGACACCTGCTCGACGACATGCAGGTGTTCGAAGACACGCCGGTCCAGGCGTCCCGGTACCTGTCTCCGCGGGTTGAGGT
GGAGGTGGGCTTCATCCTGGCCGCCGACCTACCGGGTGCGGGCTGCACCGAGGACGACGTGCTGGCGGCCACCGAGGCCC
TGGTGCCCGCCATCGAGCTGATCGACACCAGGATCAAGGATTGGCAGATCAAGATCTGCGACACCATCGCCGACAACGCC
TCGGCGGCGGGCTTCGTGTTGGGCGCGGCCCGGGTGCCGCCGGCCGATCTTGACGTCAGGGCGATCGATGCGAAGCTGAC
CCGCAACGGCGAGGTGGTGGCCGAGGGCCGCAGTGATGCGGTGTTGGGTAACCCGGCGACCGCGGTGGCTTGGCTGGCCG
GCAAGGTCGAGAGTTTCGGCGTGCGGCTGCGCAAAGGCGACATCGTGTTACCCGGGTCATGCACGTTCGCGGTCGAGGCG
CGGGCCGGCGACGAGTTTGTCGCCGACTTCACCGGGCTGGGTTTGGTCCGGTTGTCGTTCGAATAA

Upstream 100 bases:

>100_bases
CCACGACGTCGAACTCCTGCACAGTCATGTAGAACACGTTATAGAATTGCTCGGGTCGGGCGCTACTGGCCCGGTCACGC
CTACGAGGGGACTTCGCCGA

Downstream 100 bases:

>100_bases
GGGGAGCAACATGCCGTCCAAGGCAAAAGTGGCGATTGTCGGGTCGGGAAATATCAGCACCGATCTGCTGTACAAGCTGC
TGCGATCGGAGTGGCTGGAG

Product: 2-keto-4-pentenoate hydratase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 3 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDY
GHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNA
SAAGFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA
RAGDEFVADFTGLGLVRLSFE

Sequences:

>Translated_261_residues
MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDY
GHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNA
SAAGFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA
RAGDEFVADFTGLGLVRLSFE
>Mature_261_residues
MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDY
GHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNA
SAAGFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA
RAGDEFVADFTGLGLVRLSFE

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=255, Percent_Identity=42.7450980392157, Blast_Score=200, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 27892; Mature: 27892

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHH
KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA
HCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEEEECCCCC
GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCEE
AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA
EEEEEECCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHCEEEECCCEEECCCCEEEEEE
RAGDEFVADFTGLGLVRLSFE
CCCCHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHH
KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA
HCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEEEECCCCC
GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCEE
AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA
EEEEEECCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHCEEEECCCEEECCCCEEEEEE
RAGDEFVADFTGLGLVRLSFE
CCCCHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791097; 12823813 [H]