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The map label for this gene is ilvB2

Identifier: 148663334

GI number: 148663334

Start: 3895821

End: 3897479

Strand: Reverse

Name: ilvB2

Synonym: MRA_3510

Alternate gene names: 148663334

Gene position: 3897479-3895821 (Counterclockwise)

Preceding gene: 148663335

Following gene: 148663333

Centisome position: 88.18

GC content: 65.82

Gene sequence:

>1659_bases
GTGACCGTCGGGGATCATCTCGTCGCCAGGATGCGCGCAGCCGGCATTTCGGTCGTATGCGGGCTACCGACTTCCCGGCT
CGATTCGTTGCTGGTGCGTCTGTCCCGCGATGCCGGCTTTCAGATCGTGTTGGCCCGGCACGAGGGCGGCGCCGGCTACC
TGGCCGACGGGTTCGCCCGCGCGTCCGGCAAGTCCGCTGCGGTGTTTGTGGCGGGACCTGGGGCGACCAACGTCATTAGC
GCCGTGGCCAATGCGTCGGTGAATCAGGTTCCGATGCTTATCCTGACCGGGGAGGTGGCGGTCGGAGAATTCGGCCTGCA
CTCGCAGCAGGATACGAGCGACGACGGACTGGGCCTGGGCGCAACCTTCCGCCGGTTCTGCCGCTGTTCGGTGTCCATCG
AATCGATCGCCAATGCGCGCAGCAAGATCGATTCGGCGTTCCGGGCCTTGGCGAGCATCCCTCGCGGACCGGTGCACATT
GCTCTGCCGCGCGATCTAGTGGACGAGCGGCTGCCGGCTCACCAACTTGGAACTGCCGCGGCCGGTCTTGGGGGCCTAAG
AACCCTGGCGCCGTGCGGTCCTGATGTCGCTGACGAGGTGATCGGTAGGCTGGATCGGTCGCGTGCCCCCATGCTCGTGC
TGGGGAATGGCTGCCGGCTGGACGGCATCGGCGAGCAGATCGTCGCGTTTTGCGAGAAGGCGGGACTTCCTTTTGCGACC
ACACCGAACGGGCGGGGGATCGTCGCGGAAACCCATCCGCTGTCTCTGGGTGTGCTCGGCATATTTGGTGACGGCAGGGC
CGACGAGTACCTCTTCGACACACCGTGCGACCTGTTGATCGCGGTCGGTGTTTCCTTCGGCGGGTTGGTTACCCGCTCCT
TTTCGCCCCGCTGGCGCGGGCTGAAAGCCGACGTCGTCCATGTCGATCCCGATCCATCGGCAGTTGGCCGATTCGTCGCT
ACGTCGCTCGGGATCACCACGTCCGGGCGCGCGTTCGTCAATGCCCTGAACTGCGGGCGGCCGCCACGCTTTTGTCGGAG
GGTGGGCGTGCGGCCGCCGGCGCCAGCGGCGTTGCCGGGGACCCCGCAAGCGCGGGGGGAATCGATTCACCCGCTTGAGC
TGATGCACGAACTGGACCGCGAGCTTGCACCCAACGCCACGATATGCGCCGACGTGGGTACCTGCATTTCTTGGACGTTT
CGTGGGATACCGGTGCGGCGGCCCGGCAGATTCTTTGCCACCGTTGACTTTTCGCCCATGGGATGCGGCATCGCCGGAGC
CATTGGCGTGGCCCTGGCTCGACCGGAAGAACACGTCATATGCATCGCCGGGGACGGGGCTTTCTTGATGCATGGCACGG
AGATTTCCACTGCGGTTGCGCACGGGATCAGGGTGACCTGGGCTGTCCTCAACGACGGACAAATGAGTGCCAGCGCCGGC
CCGGTCAGCGGGCGGATGGATCCGTCGCCGGTCGCTCGCATCGGCGCGAACGATCTGGCGGCGATGGCCCGCGCCCTTGG
AGCGGAAGGGATTCGGGTCGATACGCGCTGCGAGCTGCGCGCCGGTGTGCAAAAAGCGTTGGCCGCCACCGGTCCCTGCG
TCCTCGATATCGCGATCGATCCAGAGATCAACAAACCCGACATTGGACTGGGTCGATAG

Upstream 100 bases:

>100_bases
TTCCGTTGACATTCTTGTGCGCCTGCGGCCCGGCCTACCTACCGGAACGCGATCAGCGCATGGGCGAGGCCGCCGTGATC
GGGGCATGGCCGTGACTCCC

Downstream 100 bases:

>100_bases
GACACCAGCCGCGCGTCCAAGAATTCATCAAGGATCCGGCAAGGGTGCGGGCTGACACTGATGCTCATGACTGCGACCCA
CAGAGAGCCGATTGTCCTGG

Product: putative acetolactate synthase large subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTVGDHLVARMRAAGISVVCGLPTSRLDSLLVRLSRDAGFQIVLARHEGGAGYLADGFARASGKSAAVFVAGPGATNVIS
AVANASVNQVPMLILTGEVAVGEFGLHSQQDTSDDGLGLGATFRRFCRCSVSIESIANARSKIDSAFRALASIPRGPVHI
ALPRDLVDERLPAHQLGTAAAGLGGLRTLAPCGPDVADEVIGRLDRSRAPMLVLGNGCRLDGIGEQIVAFCEKAGLPFAT
TPNGRGIVAETHPLSLGVLGIFGDGRADEYLFDTPCDLLIAVGVSFGGLVTRSFSPRWRGLKADVVHVDPDPSAVGRFVA
TSLGITTSGRAFVNALNCGRPPRFCRRVGVRPPAPAALPGTPQARGESIHPLELMHELDRELAPNATICADVGTCISWTF
RGIPVRRPGRFFATVDFSPMGCGIAGAIGVALARPEEHVICIAGDGAFLMHGTEISTAVAHGIRVTWAVLNDGQMSASAG
PVSGRMDPSPVARIGANDLAAMARALGAEGIRVDTRCELRAGVQKALAATGPCVLDIAIDPEINKPDIGLGR

Sequences:

>Translated_552_residues
MTVGDHLVARMRAAGISVVCGLPTSRLDSLLVRLSRDAGFQIVLARHEGGAGYLADGFARASGKSAAVFVAGPGATNVIS
AVANASVNQVPMLILTGEVAVGEFGLHSQQDTSDDGLGLGATFRRFCRCSVSIESIANARSKIDSAFRALASIPRGPVHI
ALPRDLVDERLPAHQLGTAAAGLGGLRTLAPCGPDVADEVIGRLDRSRAPMLVLGNGCRLDGIGEQIVAFCEKAGLPFAT
TPNGRGIVAETHPLSLGVLGIFGDGRADEYLFDTPCDLLIAVGVSFGGLVTRSFSPRWRGLKADVVHVDPDPSAVGRFVA
TSLGITTSGRAFVNALNCGRPPRFCRRVGVRPPAPAALPGTPQARGESIHPLELMHELDRELAPNATICADVGTCISWTF
RGIPVRRPGRFFATVDFSPMGCGIAGAIGVALARPEEHVICIAGDGAFLMHGTEISTAVAHGIRVTWAVLNDGQMSASAG
PVSGRMDPSPVARIGANDLAAMARALGAEGIRVDTRCELRAGVQKALAATGPCVLDIAIDPEINKPDIGLGR
>Mature_551_residues
TVGDHLVARMRAAGISVVCGLPTSRLDSLLVRLSRDAGFQIVLARHEGGAGYLADGFARASGKSAAVFVAGPGATNVISA
VANASVNQVPMLILTGEVAVGEFGLHSQQDTSDDGLGLGATFRRFCRCSVSIESIANARSKIDSAFRALASIPRGPVHIA
LPRDLVDERLPAHQLGTAAAGLGGLRTLAPCGPDVADEVIGRLDRSRAPMLVLGNGCRLDGIGEQIVAFCEKAGLPFATT
PNGRGIVAETHPLSLGVLGIFGDGRADEYLFDTPCDLLIAVGVSFGGLVTRSFSPRWRGLKADVVHVDPDPSAVGRFVAT
SLGITTSGRAFVNALNCGRPPRFCRRVGVRPPAPAALPGTPQARGESIHPLELMHELDRELAPNATICADVGTCISWTFR
GIPVRRPGRFFATVDFSPMGCGIAGAIGVALARPEEHVICIAGDGAFLMHGTEISTAVAHGIRVTWAVLNDGQMSASAGP
VSGRMDPSPVARIGANDLAAMARALGAEGIRVDTRCELRAGVQKALAATGPCVLDIAIDPEINKPDIGLGR

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=25.7657657657658, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=26.4388489208633, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=27.2058823529412, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=22.9656419529837, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786717, Length=487, Percent_Identity=21.7659137577002, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788716, Length=570, Percent_Identity=23.6842105263158, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531299, Length=536, Percent_Identity=24.2537313432836, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=536, Percent_Identity=24.2537313432836, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=26.3431542461005, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323073, Length=493, Percent_Identity=25.1521298174442, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=25.3036437246964, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=491, Percent_Identity=24.6435845213849, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=22.7353463587922, Blast_Score=96, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVB2_MYCTU (O06335)

Other databases:

- EMBL:   BX842583
- PIR:   C70567
- RefSeq:   NP_217987.1
- HSSP:   P07342
- ProteinModelPortal:   O06335
- SMR:   O06335
- EnsemblBacteria:   EBMYCT00000000091
- GeneID:   888041
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv3470c
- TubercuList:   Rv3470c
- GeneTree:   EBGT00050000014643
- HOGENOM:   HBG565361
- OMA:   RECELDE
- ProtClustDB:   CLSK792510
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 57257; Mature: 57126

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGDHLVARMRAAGISVVCGLPTSRLDSLLVRLSRDAGFQIVLARHEGGAGYLADGFAR
CCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHH
ASGKSAAVFVAGPGATNVISAVANASVNQVPMLILTGEVAVGEFGLHSQQDTSDDGLGLG
CCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCC
ATFRRFCRCSVSIESIANARSKIDSAFRALASIPRGPVHIALPRDLVDERLPAHQLGTAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCHHHHHHHH
AGLGGLRTLAPCGPDVADEVIGRLDRSRAPMLVLGNGCRLDGIGEQIVAFCEKAGLPFAT
HCCCCHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEE
TPNGRGIVAETHPLSLGVLGIFGDGRADEYLFDTPCDLLIAVGVSFGGLVTRSFSPRWRG
CCCCCEEEEECCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCEEECCCCCCCCC
LKADVVHVDPDPSAVGRFVATSLGITTSGRAFVNALNCGRPPRFCRRVGVRPPAPAALPG
CEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
TPQARGESIHPLELMHELDRELAPNATICADVGTCISWTFRGIPVRRPGRFFATVDFSPM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEECCCCCEEEEEECCCC
GCGIAGAIGVALARPEEHVICIAGDGAFLMHGTEISTAVAHGIRVTWAVLNDGQMSASAG
CCCHHHHHHHHEECCCCCEEEEECCCEEEEECCHHHHHHHCCEEEEEEEECCCCCCCCCC
PVSGRMDPSPVARIGANDLAAMARALGAEGIRVDTRCELRAGVQKALAATGPCVLDIAID
CCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEEC
PEINKPDIGLGR
CCCCCCCCCCCC
>Mature Secondary Structure 
TVGDHLVARMRAAGISVVCGLPTSRLDSLLVRLSRDAGFQIVLARHEGGAGYLADGFAR
CCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHH
ASGKSAAVFVAGPGATNVISAVANASVNQVPMLILTGEVAVGEFGLHSQQDTSDDGLGLG
CCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCC
ATFRRFCRCSVSIESIANARSKIDSAFRALASIPRGPVHIALPRDLVDERLPAHQLGTAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCHHHHHHHH
AGLGGLRTLAPCGPDVADEVIGRLDRSRAPMLVLGNGCRLDGIGEQIVAFCEKAGLPFAT
HCCCCHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEE
TPNGRGIVAETHPLSLGVLGIFGDGRADEYLFDTPCDLLIAVGVSFGGLVTRSFSPRWRG
CCCCCEEEEECCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHCCCCCEEECCCCCCCCC
LKADVVHVDPDPSAVGRFVATSLGITTSGRAFVNALNCGRPPRFCRRVGVRPPAPAALPG
CEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
TPQARGESIHPLELMHELDRELAPNATICADVGTCISWTFRGIPVRRPGRFFATVDFSPM
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEECCCCCEEEEEECCCC
GCGIAGAIGVALARPEEHVICIAGDGAFLMHGTEISTAVAHGIRVTWAVLNDGQMSASAG
CCCHHHHHHHHEECCCCCEEEEECCCEEEEECCHHHHHHHCCEEEEEEEECCCCCCCCCC
PVSGRMDPSPVARIGANDLAAMARALGAEGIRVDTRCELRAGVQKALAATGPCVLDIAID
CCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEEC
PEINKPDIGLGR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230