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The map label for this gene is mhpE [H]

Identifier: 148663333

GI number: 148663333

Start: 3894750

End: 3895760

Strand: Reverse

Name: mhpE [H]

Synonym: MRA_3509

Alternate gene names: 148663333

Gene position: 3895760-3894750 (Counterclockwise)

Preceding gene: 148663334

Following gene: 148663332

Centisome position: 88.14

GC content: 63.2

Gene sequence:

>1011_bases
ATGCTCATGACTGCGACCCACAGAGAGCCGATTGTCCTGGACACGACCGTCCGTGACGGCAGCTATGCGGTGAACTTCCA
GTACACCGACGACGATGTCCGCAGGATTGTCGGGGATCTCGACGCAGCGGGAATTCCCTACATTGAAATCGGTCACGGCG
TCACCATCGGAGCCGCCGCGGCGCAGGGCCCGGCGGCTCACACCGACGAAGAATATTTCCGCGCCGCCCGATCGGTGGTT
CGAAACGCGAGACTTGGTGCCGTGATCGTGCCGGCCCTGGCCCGCATCGAGACGGTCGATTTGGCCGGAGACTATCTGGA
CTTTCTGCGCATATGTGTCATCGCCACCGAATTTGAGTTGGTGATGCCGTTCGTCGAGCGTGCGCAGTCAAAAGGTCTGG
AGGTTTCGATCCAGCTGGTGAAGTCGCATCTCTTCGAGCCCGATGTGCTTGCTGCTGCCGGGAAGCGGGCCCGCGATGTG
GGCGTCCGGATCGTTTACGTGGTGGATACGACGGGAACCTTCCTGCCCGAGGACGCGCGCCGCTACGTCGAGGCTCTCCG
CGGTGCCAGCGATGTCTCCGTCGGCTTCCACGGGCACAACAACCTCGCCATGGCCGTCGCCAACACCTTGGAGGCATTCG
ACGCCGGGGCCGATTTCCTGGATGGAACGCTGATGGGTTTCGGTCGCGGGGCCGGGAATTGCCAGATCGAGTGCCTCGTC
GCCGCGTTGCAGCGGCGCGGGCACCTGGCGGCAGTCGACCTGGATCGGATTTTCGACGCAGCGCGATCGGATATGTTGGG
CCGGAGCCCGCAGAGTTACGGCATCGATCCGTGGGAGATCTCGTTCGGATTCCACGGACTCGATTCGCTGCAGGTCGAGC
ACCTCCGCGCAGCGGCCCAACAGGCGGGGCTTTCCGTGTCCCACGTTATTCGGCAGACCGCGAAAAGTCATGCGGGGCAG
TGGTTGTCCCCGCAAGACATCGATCGAGTGGTCGTGGGAATGAGGGCGTGA

Upstream 100 bases:

>100_bases
TCCAGAGATCAACAAACCCGACATTGGACTGGGTCGATAGGACACCAGCCGCGCGTCCAAGAATTCATCAAGGATCCGGC
AAGGGTGCGGGCTGACACTG

Downstream 100 bases:

>100_bases
GTCGTGGGAACACATGCAGCCACCATGAGAGTCCGCGCCGGCGTGCGGTCCAGTCCGCTGTTGTTGCACGCGGGCACGCC
TCCGACGGCTGCCGCCGCCG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAAAQGPAAHTDEEYFRAARSVV
RNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFELVMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDV
GVRIVYVVDTTGTFLPEDARRYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLV
AALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQQAGLSVSHVIRQTAKSHAGQ
WLSPQDIDRVVVGMRA

Sequences:

>Translated_336_residues
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAAAQGPAAHTDEEYFRAARSVV
RNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFELVMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDV
GVRIVYVVDTTGTFLPEDARRYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLV
AALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQQAGLSVSHVIRQTAKSHAGQ
WLSPQDIDRVVVGMRA
>Mature_336_residues
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAAAQGPAAHTDEEYFRAARSVV
RNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFELVMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDV
GVRIVYVVDTTGTFLPEDARRYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLV
AALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQQAGLSVSHVIRQTAKSHAGQ
WLSPQDIDRVVVGMRA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=314, Percent_Identity=30.2547770700637, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36298; Mature: 36298

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAA
CCEECCCCCCEEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCEEEECCCEEEECCC
AQGPAAHTDEEYFRAARSVVRNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFEL
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHH
VMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDVGVRIVYVVDTTGTFLPEDAR
HHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHH
RYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLV
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCHHHHHH
AALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQ
HHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
QAGLSVSHVIRQTAKSHAGQWLSPQDIDRVVVGMRA
HHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAA
CCEECCCCCCEEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCEEEECCCEEEECCC
AQGPAAHTDEEYFRAARSVVRNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFEL
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHH
VMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDVGVRIVYVVDTTGTFLPEDAR
HHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHH
RYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLV
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCHHHHHH
AALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQ
HHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
QAGLSVSHVIRQTAKSHAGQWLSPQDIDRVVVGMRA
HHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA