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The map label for this gene is murD [H]

Identifier: 148661971

GI number: 148661971

Start: 2424836

End: 2426296

Strand: Reverse

Name: murD [H]

Synonym: MRA_2170

Alternate gene names: 148661971

Gene position: 2426296-2424836 (Counterclockwise)

Preceding gene: 148661972

Following gene: 148661970

Centisome position: 54.89

GC content: 70.5

Gene sequence:

>1461_bases
GTGCTTGACCCTCTGGGGCCGGGTGCGCCCGTGTTGGTAGCCGGTGGCCGGGTGACCGGTCAGGCGGTGGCCGCGGTGCT
GACTCGGTTTGGTGCGACGCCGACGGTGTGCGACGACGATCCGGTCATGCTGCGACCGCACGCCGAACGTGGGCTGCCGA
CCGTTAGTTCCTCGGACGCGGTGCAGCAGATAACCGGGTATGCGCTGGTGGTCGCCAGTCCCGGCTTCTCGCCCGCAACC
CCGCTACTGGCCGCGGCCGCGGCGGCGGGGGTGCCGATCTGGGGTGACGTGGAGTTAGCCTGGCGGCTAGACGCAGCGGG
CTGCTACGGACCGCCGCGCAGCTGGCTGGTGGTGACCGGCACCAACGGCAAGACCACCACGACGTCGATGCTGCACGCCA
TGCTGATCGCCGGTGGCCGCCGCGCCGTGCTGTGCGGCAATATCGGCAGTGCGGTGCTGGATGTGCTGGACGAGCCGGCC
GAGCTGCTGGCCGTGGAGTTGTCCAGTTTCCAGCTGCACTGGGCGCCGTCGCTGCGGCCCGAGGCCGGCGCGGTGCTCAA
CATTGCCGAAGACCACCTGGACTGGCATGCCACGATGGCCGAATACACCGCGGCCAAGGCCCGGGTGCTGACCGGCGGGG
TAGCGGTGGCCGGGCTGGATGACAGCCGAGCGGCCGCACTGCTGGACGGCTCACCGGCGCAGGTGCGGGTCGGCTTCCGG
CTCGGCGAGCCGGCCGCGCGGGAACTGGGCGTGCGCGACGCCCACCTGGTCGATCGCGCCTTCTCCGACGACTTGACGCT
GCTGCCGGTCGCGTCGATACCGGTGCCAGGTCCGGTCGGCGTGCTTGACGCCCTGGCCGCGGCGGCGCTGGCCCGCTCGG
TCGGGGTGCCCGCCGGTGCGATCGCCGACGCGGTCACGTCGTTTCGAGTGGGCCGACACCGCGCCGAGGTGGTGGCCGTT
GCCGACGGCATCACCTACGTGGACGACTCCAAGGCCACCAACCCGCACGCCGCGCGGGCTTCGGTGCTTGCATACCCGAG
GGTGGTATGGATCGCCGGTGGCCTGCTCAAGGGCGCGTCGCTTCACGCCGAGGTTGCGGCGATGGCGTCGCGGCTGGTCG
GTGCGGTGCTGATCGGCCGGGATCGCGCAGCGGTTGCCGAGGCGTTATCACGACACGCGCCCGATGTCCCAGTCGTTCAG
GTTGTGGCAGGCGAGGATACTGGTATGCCTGCGACTGTTGAGGTTCCTGTTGCTTGTGTTCTAGATGTGGCAAAAGATGA
CAAAGCCGGTGAGACCGTTGGCGCTGCCGTGATGACCGCTGCGGTGGCCGCGGCCCGGCGGATGGCCCAACCCGGTGACA
CCGTGCTGCTGGCACCGGCCGGCGCCTCATTCGACCAGTTCACCGGTTATGCCGACCGGGGCGAGGCATTCGCGACCGCG
GTCCGCGCGGTGATCCGGTAG

Upstream 100 bases:

>100_bases
ACCACGGTCATCATCCGGTTCTGGCTGCTCACCGCGATCACCTGCGGTCTGGGCGTGGCCTTGTTCTACGGTGAGTGGCT
TGCCGCGGTCGGTGCCTGAC

Downstream 100 bases:

>100_bases
GCGGGGGCAGCGTGCTAACCCGGTTGCTGCGTCGGGGCACCAGCGACACCGACGGCTCCCAGACTCGAGGGGCCGAGCCG
GTCGAGGGGCAGCGGACGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDAVQQITGYALVVASPGFSPAT
PLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLVVTGTNGKTTTTSMLHAMLIAGGRRAVLCGNIGSAVLDVLDEPA
ELLAVELSSFQLHWAPSLRPEAGAVLNIAEDHLDWHATMAEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFR
LGEPAARELGVRDAHLVDRAFSDDLTLLPVASIPVPGPVGVLDALAAAALARSVGVPAGAIADAVTSFRVGRHRAEVVAV
ADGITYVDDSKATNPHAARASVLAYPRVVWIAGGLLKGASLHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPVVQ
VVAGEDTGMPATVEVPVACVLDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFDQFTGYADRGEAFATA
VRAVIR

Sequences:

>Translated_486_residues
MLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDAVQQITGYALVVASPGFSPAT
PLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLVVTGTNGKTTTTSMLHAMLIAGGRRAVLCGNIGSAVLDVLDEPA
ELLAVELSSFQLHWAPSLRPEAGAVLNIAEDHLDWHATMAEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFR
LGEPAARELGVRDAHLVDRAFSDDLTLLPVASIPVPGPVGVLDALAAAALARSVGVPAGAIADAVTSFRVGRHRAEVVAV
ADGITYVDDSKATNPHAARASVLAYPRVVWIAGGLLKGASLHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPVVQ
VVAGEDTGMPATVEVPVACVLDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFDQFTGYADRGEAFATA
VRAVIR
>Mature_486_residues
MLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDAVQQITGYALVVASPGFSPAT
PLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLVVTGTNGKTTTTSMLHAMLIAGGRRAVLCGNIGSAVLDVLDEPA
ELLAVELSSFQLHWAPSLRPEAGAVLNIAEDHLDWHATMAEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFR
LGEPAARELGVRDAHLVDRAFSDDLTLLPVASIPVPGPVGVLDALAAAALARSVGVPAGAIADAVTSFRVGRHRAEVVAV
ADGITYVDDSKATNPHAARASVLAYPRVVWIAGGLLKGASLHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPVVQ
VVAGEDTGMPATVEVPVACVLDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFDQFTGYADRGEAFATA
VRAVIR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=488, Percent_Identity=31.7622950819672, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49346; Mature: 49346

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCHHCCCCCCCCCHH
VQQITGYALVVASPGFSPATPLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLVVTG
HHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCCEEEEEC
TNGKTTTTSMLHAMLIAGGRRAVLCGNIGSAVLDVLDEPAELLAVELSSFQLHWAPSLRP
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCHHHEEEECCCEEEEECCCCCC
EAGAVLNIAEDHLDWHATMAEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFR
CCCCEEEEHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCEEEECCCCCEEEEEEE
LGEPAARELGVRDAHLVDRAFSDDLTLLPVASIPVPGPVGVLDALAAAALARSVGVPAGA
ECCCHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHH
IADAVTSFRVGRHRAEVVAVADGITYVDDSKATNPHAARASVLAYPRVVWIAGGLLKGAS
HHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHCCCC
LHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPVVQVVAGEDTGMPATVEVPVACV
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEE
LDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFDQFTGYADRGEAFATA
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHH
VRAVIR
HHHHHC
>Mature Secondary Structure
MLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCHHCCCCCCCCCHH
VQQITGYALVVASPGFSPATPLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLVVTG
HHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCCEEEEEC
TNGKTTTTSMLHAMLIAGGRRAVLCGNIGSAVLDVLDEPAELLAVELSSFQLHWAPSLRP
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCHHHEEEECCCEEEEECCCCCC
EAGAVLNIAEDHLDWHATMAEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFR
CCCCEEEEHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCEEEECCCCCEEEEEEE
LGEPAARELGVRDAHLVDRAFSDDLTLLPVASIPVPGPVGVLDALAAAALARSVGVPAGA
ECCCHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHH
IADAVTSFRVGRHRAEVVAVADGITYVDDSKATNPHAARASVLAYPRVVWIAGGLLKGAS
HHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHCCCC
LHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPVVQVVAGEDTGMPATVEVPVACV
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEE
LDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFDQFTGYADRGEAFATA
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHH
VRAVIR
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]