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The map label for this gene is hisF [H]

Identifier: 148661400

GI number: 148661400

Start: 1806371

End: 1807174

Strand: Direct

Name: hisF [H]

Synonym: MRA_1615

Alternate gene names: 148661400

Gene position: 1806371-1807174 (Clockwise)

Preceding gene: 148661399

Following gene: 148661401

Centisome position: 40.87

GC content: 68.16

Gene sequence:

>804_bases
ATGTATGCCGACCGTGACCTTCCGGGGGCTGGGGGCCTCGCGGTACGCGTGATCCCGTGTCTGGATGTCGACGATGGGCG
GGTGGTCAAGGGAGTCAACTTCGAGAACCTCCGCGACGCCGGTGATCCCGTGGAACTCGCCGCCGTCTATGACGCGGAGG
GCGCGGACGAGTTGACCTTTCTCGACGTGACCGCGTCGTCGTCCGGAAGAGCCACCATGCTGGAGGTGGTGCGCCGCACC
GCCGAGCAGGTGTTCATCCCGCTGACGGTGGGCGGTGGGGTACGCACCGTCGCCGACGTCGATTCGCTGCTACGGGCTGG
GGCTGACAAAGTCGCCGTCAACACGGCCGCCATCGCTTGCCCGGACTTGCTGGCGGACATGGCGAGGCAGTTCGGCTCGC
AGTGCATCGTGTTGTCCGTCGACGCGCGCACAGTTCCGGTGGGATCAGCCCCGACACCGTCGGGTTGGGAGGTCACCACT
CACGGCGGTCGTCGTGGCACCGGTATGGACGCCGTGCAGTGGGCGGCCCGTGGCGCCGACCTCGGTGTGGGGGAGATCCT
GCTCAACTCGATGGACGCCGACGGCACCAAAGCCGGATTCGACCTGGCTTTGCTGCGTGCGGTCCGTGCCGCGGTCACGG
TGCCGGTAATCGCCAGCGGGGGCGCCGGTGCTGTGGAGCACTTCGCGCCAGCGGTTGCCGCGGGGGCCGATGCAGTGTTG
GCGGCCAGCGTCTTTCACTTCCGGGAGCTGACGATCGGTCAGGTGAAGGCGGCCCTGGCCGCGGAAGGAATCACCGTGCG
ATGA

Upstream 100 bases:

>100_bases
TGGACCCCTGCATCGCGTTCTGCCTTGGCCGGGCCACCGCGCGTGCATGCCCAGATCCTCGAGATTCTTGGCAGCATAGG
GGAACCAGAGGACTACTGAG

Downstream 100 bases:

>100_bases
CACTCGACCCAAAGATCGCGGCGCGGTTGAAGCGTAATGCCGACGGACTGGTTACCGCCGTCGTCCAGGAGCGGGGCAGC
GGTGACGTGCTGATGGTTGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACPDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR

Sequences:

>Translated_267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACPDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR
>Mature_267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACPDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=45.3846153846154, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=28.099173553719, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27221; Mature: 27221

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTF
CCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEE
LDVTASSSGRATMLEVVRRTAEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIAC
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEECHHHHHH
PDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTTHGGRRGTGMDAVQWAARGAD
HHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCC
LGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
AASVFHFRELTIGQVKAALAAEGITVR
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTF
CCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEE
LDVTASSSGRATMLEVVRRTAEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIAC
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEECHHHHHH
PDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTTHGGRRGTGMDAVQWAARGAD
HHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCC
LGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
AASVFHFRELTIGQVKAALAAEGITVR
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]