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The map label for this gene is impA

Identifier: 148661399

GI number: 148661399

Start: 1805557

End: 1806369

Strand: Direct

Name: impA

Synonym: MRA_1614

Alternate gene names: 148661399

Gene position: 1805557-1806369 (Clockwise)

Preceding gene: 148661398

Following gene: 148661400

Centisome position: 40.85

GC content: 65.07

Gene sequence:

>813_bases
ATGCACTTGGATTCGTTGGTTGCCCCGCTGGTTGAACAGGCGTCGGCGATCCTGGATGCCGCAACGGCGCTCTTTCTCGT
CGGTCATCGCGCCGATTCAGCGGTCCGCAAGAAGGGTAACGACTTCGCCACCGAAGTCGATCTAGCGATCGAGCGGCAGG
TTGTCGCAGCGCTGGTGGCGGCCACCGGCATCGAGGTGCACGGCGAGGAGTTCGGCGGCCCGGCAGTCGACTCGCGGTGG
GTGTGGGTACTGGACCCCATCGACGGCACAATCAACTACGCCGCCGGATCGCCGTTGGCTGCGATCCTGTTGGGCCTGCT
GCACGACGGAGTTCCGGTGGCCGGCTTGACCTGGATGCCATTCACCGACCCACGCTATACCGCCGTGGCGGGTGGTCCGC
TGATCAAGAACGGTGTACCGCAGCCGCCGCTGGCTGACGCCGAACTGGCCAACGTGCTCGTCGGCGTCGGCACATTCAGC
GCCGACTCACGGGGCCAGTTCCCGGGGCGATATCGACTGGCGGTGCTGGAAAAGCTCAGCCGAGTGTCATCGCGGCTGCG
CATGCACGGATCCACCGGCATCGATCTCGTCTTCGTCGCTGACGGGATACTCGGTGGTGCAATAAGTTTCGGAGGTCACG
TTTGGGACCATGCCGCTGGGGTGGCGTTGGTACGAGCCGCCGGTGGCGTGGTCACCGACCTGGCTGGGCAACCGTGGACC
CCTGCATCGCGTTCTGCCTTGGCCGGGCCACCGCGCGTGCATGCCCAGATCCTCGAGATTCTTGGCAGCATAGGGGAACC
AGAGGACTACTGA

Upstream 100 bases:

>100_bases
ACGCACCGCGGCGTCGAGGGGGCCATCGTCGGCAAGGCCCTCTACGCCCGTCGGTTCACCTTGCCGCAAGCGTTGGCCGC
GGTTCGGGACTAGATCGGCG

Downstream 100 bases:

>100_bases
GATGTATGCCGACCGTGACCTTCCGGGGGCTGGGGGCCTCGCGGTACGCGTGATCCCGTGTCTGGATGTCGACGATGGGC
GGGTGGTCAAGGGAGTCAAC

Product: inositol-monophosphatase ImpA

Products: NA

Alternate protein names: I-1-Pase; IMPase; Inositol-1-phosphatase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHLDSLVAPLVEQASAILDAATALFLVGHRADSAVRKKGNDFATEVDLAIERQVVAALVAATGIEVHGEEFGGPAVDSRW
VWVLDPIDGTINYAAGSPLAAILLGLLHDGVPVAGLTWMPFTDPRYTAVAGGPLIKNGVPQPPLADAELANVLVGVGTFS
ADSRGQFPGRYRLAVLEKLSRVSSRLRMHGSTGIDLVFVADGILGGAISFGGHVWDHAAGVALVRAAGGVVTDLAGQPWT
PASRSALAGPPRVHAQILEILGSIGEPEDY

Sequences:

>Translated_270_residues
MHLDSLVAPLVEQASAILDAATALFLVGHRADSAVRKKGNDFATEVDLAIERQVVAALVAATGIEVHGEEFGGPAVDSRW
VWVLDPIDGTINYAAGSPLAAILLGLLHDGVPVAGLTWMPFTDPRYTAVAGGPLIKNGVPQPPLADAELANVLVGVGTFS
ADSRGQFPGRYRLAVLEKLSRVSSRLRMHGSTGIDLVFVADGILGGAISFGGHVWDHAAGVALVRAAGGVVTDLAGQPWT
PASRSALAGPPRVHAQILEILGSIGEPEDY
>Mature_270_residues
MHLDSLVAPLVEQASAILDAATALFLVGHRADSAVRKKGNDFATEVDLAIERQVVAALVAATGIEVHGEEFGGPAVDSRW
VWVLDPIDGTINYAAGSPLAAILLGLLHDGVPVAGLTWMPFTDPRYTAVAGGPLIKNGVPQPPLADAELANVLVGVGTFS
ADSRGQFPGRYRLAVLEKLSRVSSRLRMHGSTGIDLVFVADGILGGAISFGGHVWDHAAGVALVRAAGGVVTDLAGQPWT
PASRSALAGPPRVHAQILEILGSIGEPEDY

Specific function: Catalyzes the dephosphorylation of inositol-1-phosphate (I-1-P) to yield free myo-inositol, a key metabolite in mycobacteria

COG id: COG0483

COG function: function code G; Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inositol monophosphatase family

Homologues:

Organism=Homo sapiens, GI5031789, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221625487, Length=176, Percent_Identity=28.4090909090909, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI7657236, Length=179, Percent_Identity=27.3743016759777, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788882, Length=231, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193202570, Length=259, Percent_Identity=31.6602316602317, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193202572, Length=258, Percent_Identity=30.6201550387597, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320493, Length=207, Percent_Identity=28.0193236714976, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21357329, Length=270, Percent_Identity=29.2592592592593, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357303, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IMPA_MYCTU (O53907)

Other databases:

- EMBL:   BX842577
- PIR:   C70819
- RefSeq:   NP_216120.1
- ProteinModelPortal:   O53907
- SMR:   O53907
- EnsemblBacteria:   EBMYCT00000001523
- GeneID:   885567
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv1604
- TubercuList:   Rv1604
- GeneTree:   EBGT00050000015694
- HOGENOM:   HBG730251
- OMA:   CTELTRT
- ProtClustDB:   CLSK791276
- InterPro:   IPR000760
- InterPro:   IPR020550
- PANTHER:   PTHR20854
- PRINTS:   PR00377

Pfam domain/function: PF00459 Inositol_P

EC number: =3.1.3.25

Molecular weight: Translated: 27979; Mature: 27979

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00629 IMP_1; PS00630 IMP_2

Important sites: BINDING 69-69 BINDING 187-187 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLDSLVAPLVEQASAILDAATALFLVGHRADSAVRKKGNDFATEVDLAIERQVVAALVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ATGIEVHGEEFGGPAVDSRWVWVLDPIDGTINYAAGSPLAAILLGLLHDGVPVAGLTWMP
HHCCEECCHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEECC
FTDPRYTAVAGGPLIKNGVPQPPLADAELANVLVGVGTFSADSRGQFPGRYRLAVLEKLS
CCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RVSSRLRMHGSTGIDLVFVADGILGGAISFGGHVWDHAAGVALVRAAGGVVTDLAGQPWT
HHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PASRSALAGPPRVHAQILEILGSIGEPEDY
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHLDSLVAPLVEQASAILDAATALFLVGHRADSAVRKKGNDFATEVDLAIERQVVAALVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ATGIEVHGEEFGGPAVDSRWVWVLDPIDGTINYAAGSPLAAILLGLLHDGVPVAGLTWMP
HHCCEECCHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEECC
FTDPRYTAVAGGPLIKNGVPQPPLADAELANVLVGVGTFSADSRGQFPGRYRLAVLEKLS
CCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RVSSRLRMHGSTGIDLVFVADGILGGAISFGGHVWDHAAGVALVRAAGGVVTDLAGQPWT
HHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PASRSALAGPPRVHAQILEILGSIGEPEDY
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230