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The map label for this gene is fadD24 [H]

Identifier: 148661325

GI number: 148661325

Start: 1731019

End: 1732773

Strand: Direct

Name: fadD24 [H]

Synonym: MRA_1541

Alternate gene names: 148661325

Gene position: 1731019-1732773 (Clockwise)

Preceding gene: 148661321

Following gene: 148661326

Centisome position: 39.16

GC content: 61.94

Gene sequence:

>1755_bases
GTGGTCGCGTCTTCGATTCCCACCGCGCTGCGCGAGCGCGCCAGTGTGCACCCCAATGGTGCGGCCATCACCTACATCGA
TTACGAGCAGGACTGGGCCGGTGTTGCCGAAACCCTGACCTGGTCTCAGTTGTATCGGCGAATGCTCAATGTCGCCGAGC
CGCTCCGGCATGTGGGGGCGACCGGTGATCGGGCAGTGATACTGGCACCGCAGGGAATCGAATACGTCGTTGGATTTCTC
GGCGCGTTGCAGGCCGGACGTATCGCGGTTCCGCTGCCGGTTCCACATGCCGGCGCCCACGATGAGCGTACGATTTCGGT
GCTAAGCGACACTTCGCCCGCTGTCATTCTGACGACGTCGGGGGCCGTTGACGATGTCAGAGAATGCGCTCAGCCACAGC
CAGGCCAGTCCGCACCATCAATCGTTGAGCTTGATTTGCTGGACTTAGATTCTCGGCAGCGCTCCCGCAGCCCTGGCGCG
CGCCCAACCGGCAGGGATACGCCGGAAACCGCGTATTTGCAATATACTTCGGGATCCACCCGTACGCCGGCCGGTGTCAT
GGTCTCGAACAAAAATGTCTTCGCCAATTTCGAGCAGATCGTGGCCGACTTCTTTGCGCCCGAGGGGGGCGTCGTCCCGC
CGGACCTCACTGTGGTGTCTTGGCTGCCGCTGTACCACGACATGGGTCTTCTATTAGGCGCGATCATGCCGATCCTGGCG
GGTGTACCCACCGTGTTGACGAGTCCGGTGGGGTTCCTTCAGCGGCCGGCTCGATGGATACAACTGCTGGCACGTAACGG
TCGCACGATTTCGGCAGGACCGAATTTCGCTTTCGAATTGGCGGTGCGTAAGACGTCAGACGACGACATGGACGGACTTG
ACCTCGCCGGCGTGCACACCATCCTCAACGGCAGCGAGCGAGTACACCCGGCGACCCTCAAACGATTTGCTGAACGGTTC
GGCCGCTTTAATTTTGCCGCCGCGGCGCTGCGGCCCGCGTATGGCATGGCGGAAGCAACGGTGTACATAGCGACCCGTAA
TGTGAACGAACCACCAGAAATCGTCGACTTCGAATCCGAGAAACTGCCTGCGGGCCAAGCGATCCGGTGCCCGAGCGGAA
GCGGCACACCGCTGGTCAGCTACGGCGTCCCACGGTCACAGCTAGTGCGCATCGTTGATCCAGACACGTGTATCGAGTGT
CCGCAGGGATCGGTCGGTGAGATCTGGGTGCAAGGTGGCAACGTTGCGTCCGGCTATTGGCACAAACCCGAGGAGAGCAA
GCGCACGTTTGGCGCCAGGATTGTCACCCCTTCGGCGGGCACACCCGAAGCGCCTTGGCTGCGAACCGGGGATTCGGGTT
TCGTCTCCGGCGGCGAGCTGTTCATCATCGGCCGCATCAAGGACCTCTTGATTGTGTATGGGCGCAACCACGCTCCCGAC
GACATCGAGGCGACCATCCAGGAGATAACCTCCGGCCGCTGTGCGGCGATCGCGGTCCCCGACCACGGCACCGAAAAGCT
GGTCGCGATTATCGAACTCAAGAAACGGGGAGACTCCGACGAGGATGTGGCGGACCGGCTGCGCATCGTCAAGCGTGACG
TCGCCGCGGCGATATTTGATTCGCACGGTCTGAGCGTGGCCGATCTCGTTCTGGTGTCGCCCGGGTCGATTCCCATCACC
ACCAGCGGCAAGATCAGGCGGGCACAGTGCGTCCAGCTTTACCGACGGCGTGAGTTCACCCGGTTAGACGCTTGA

Upstream 100 bases:

>100_bases
TCTCTCTTTGCTGCCACCAGCGCCAGGTTGGTTGTGGAAGCTGAGTCACCGTCGGGCGAAACCGTCAGCGTTGACGAAGC
GTTAGAGGTAGTGTGCTGCC

Downstream 100 bases:

>100_bases
CTGCATCGTTGGAGCTTGTTTTCCATTGTGCTACAACCGGTTTGCTGTCTCTGTGGCCCAGTGTTAGTGGGCCGCTCGGC
ATTGACTGAGCACGACACGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGATGDRAVILAPQGIEYVVGFL
GALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTSGAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGA
RPTGRDTPETAYLQYTSGSTRTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILA
GVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHTILNGSERVHPATLKRFAERF
GRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESEKLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIEC
PQGSVGEIWVQGGNVASGYWHKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPD
DIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFDSHGLSVADLVLVSPGSIPIT
TSGKIRRAQCVQLYRRREFTRLDA

Sequences:

>Translated_584_residues
MVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGATGDRAVILAPQGIEYVVGFL
GALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTSGAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGA
RPTGRDTPETAYLQYTSGSTRTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILA
GVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHTILNGSERVHPATLKRFAERF
GRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESEKLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIEC
PQGSVGEIWVQGGNVASGYWHKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPD
DIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFDSHGLSVADLVLVSPGSIPIT
TSGKIRRAQCVQLYRRREFTRLDA
>Mature_584_residues
MVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGATGDRAVILAPQGIEYVVGFL
GALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTSGAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGA
RPTGRDTPETAYLQYTSGSTRTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILA
GVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHTILNGSERVHPATLKRFAERF
GRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESEKLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIEC
PQGSVGEIWVQGGNVASGYWHKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPD
DIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFDSHGLSVADLVLVSPGSIPIT
TSGKIRRAQCVQLYRRREFTRLDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=624, Percent_Identity=25.1602564102564, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI44888818, Length=595, Percent_Identity=24.2016806722689, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI225735629, Length=570, Percent_Identity=24.3859649122807, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI45827692, Length=570, Percent_Identity=24.3859649122807, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1788107, Length=521, Percent_Identity=25.5278310940499, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=508, Percent_Identity=23.2283464566929, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=485, Percent_Identity=24.3298969072165, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193202679, Length=537, Percent_Identity=23.6499068901304, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=553, Percent_Identity=22.4231464737794, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654656, Length=486, Percent_Identity=21.8106995884774, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62830; Mature: 62830

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGA
CCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGDRAVILAPQGIEYVVGFLGALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTS
CCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEC
GAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGARPTGRDTPETAYLQYTSGST
CCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCC
RTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILA
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHT
CCCHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCCCCCCHHHHHH
ILNGSERVHPATLKRFAERFGRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESE
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
KLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIECPQGSVGEIWVQGGNVASGYW
CCCCCCEEECCCCCCCEEEEECCCHHHEEEEECCCHHCCCCCCCCCEEEEECCCEECCCC
HKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPD
CCCCHHHHCCCCEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEEECEEEEEECCCCCCC
DIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFD
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SHGLSVADLVLVSPGSIPITTSGKIRRAQCVQLYRRREFTRLDA
CCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGA
CCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGDRAVILAPQGIEYVVGFLGALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTS
CCCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEC
GAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGARPTGRDTPETAYLQYTSGST
CCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCC
RTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILA
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHT
CCCHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCCCCCCHHHHHH
ILNGSERVHPATLKRFAERFGRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESE
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
KLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIECPQGSVGEIWVQGGNVASGYW
CCCCCCEEECCCCCCCEEEEECCCHHHEEEEECCCHHCCCCCCCCCEEEEECCCEECCCC
HKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPD
CCCCHHHHCCCCEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEEECEEEEEECCCCCCC
DIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFD
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SHGLSVADLVLVSPGSIPITTSGKIRRAQCVQLYRRREFTRLDA
CCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]