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The map label for this gene is ubiE [C]

Identifier: 148661196

GI number: 148661196

Start: 1582455

End: 1583279

Strand: Reverse

Name: ubiE [C]

Synonym: MRA_1414

Alternate gene names: 148661196

Gene position: 1583279-1582455 (Counterclockwise)

Preceding gene: 148661201

Following gene: 148661194

Centisome position: 35.82

GC content: 65.33

Gene sequence:

>825_bases
ATGACTATCGACACACCTGCTCGCGAGGACCAAACGCTTGCGGCCACACACCGGGCGATGTGGGCCCTGGGTGACTACGC
CCTGATGGCAGAAGAGGTGATGGCGCCACTCGGCCCGATCCTGGTTGCCGCCGCGGGCATCGGGCCGGGTGTTCGGGTGC
TCGACGTCGCCGCTGGCTCTGGCAATATTTCGCTGCCCGCAGCCAAGACGGGTGCCACGGTCATTTCCACCGACCTGACG
CCCGAGCTGCTGCAGCGGTCCCAGGCCAGGGCCGCCCAACAGGGGCTGACCCTGCAATACCAGGAAGCCAACGCGCAAGC
CCTGCCGTTTGCCGACGACGAGTTCGACACGGTGATCTCAGCGATCGGCGTGATGTTCGCACCGGATCATCAGGCCGCGG
CCGACGAGCTGGTCCGCGTCTGCCGGCCTGGCGGGACGATCGGTGTGATCAGCTGGACGTGCGAGGGATTCTTCGGCCGG
ATGCTGGCAACCATTAGGCCATACCGGCCCAGCGTGTCGGCGGATCTGCCGCCGTCGGCGTTGTGGGGACGTGAGGCCTA
TGTCACTGGTCTGCTGGGCGATGGAGTCACTGGCCTCAAGACGGCCCGTGGCTTGTTGGAAGTGAAGCGGTTCGACACCG
CCCAGGCCGTCCACGACTACTTCAAGAACAACTATGGCCCGACGATCGAGGCCTACGCGCACATCGGTGACAACGCGGTG
CTGGCCGCCGAGCTCGACCGCCAGCTTGTCGAACTCGCAGCGCAATACCTGTCGGACGGTGTCATGGAATGGGAGTACCT
GCTGCTCACCGCCGAAAAGCGCTGA

Upstream 100 bases:

>100_bases
CCGGTGGGTACGCAAGGCACGCTACTGCCCAGGCTCCCGCCGTGCCCGCGACAACGATGTCGAAGTTCGCAGCCACATCA
CTTACTTCTCAGGAGACCTG

Downstream 100 bases:

>100_bases
GGCGGGCAATCCGGCCTGTGTCGGGTCGTCCCAGCACGTAACGGACGGCCCGACACGGGCTCGGCCGCTAGCTACTCGAT
TTCGTAGCGTGCGTCGGGCT

Product: putative methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGSGNISLPAAKTGATVISTDLT
PELLQRSQARAAQQGLTLQYQEANAQALPFADDEFDTVISAIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGR
MLATIRPYRPSVSADLPPSALWGREAYVTGLLGDGVTGLKTARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAV
LAAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR

Sequences:

>Translated_274_residues
MTIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGSGNISLPAAKTGATVISTDLT
PELLQRSQARAAQQGLTLQYQEANAQALPFADDEFDTVISAIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGR
MLATIRPYRPSVSADLPPSALWGREAYVTGLLGDGVTGLKTARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAV
LAAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR
>Mature_273_residues
TIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGSGNISLPAAKTGATVISTDLTP
ELLQRSQARAAQQGLTLQYQEANAQALPFADDEFDTVISAIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGRM
LATIRPYRPSVSADLPPSALWGREAYVTGLLGDGVTGLKTARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAVL
AAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR

Specific function: Methyltransferase Required For The Conversion Of Dimethylmenaquinone (Dmkh2) To Menaquinone (Mkh2) And The Conversion Of 2-Polyprenyl-6-Methoxy-1,4-Benzoquinol (Ddmqh2) To 2-Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol (Dmqh2). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.tuberculosis Rv1403c

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1405_MYCTU (P64841)

Other databases:

- EMBL:   BX842576
- EMBL:   AE000516
- PIR:   B70901
- RefSeq:   NP_215921.1
- RefSeq:   NP_335900.1
- ProteinModelPortal:   P64841
- SMR:   P64841
- EnsemblBacteria:   EBMYCT00000000300
- EnsemblBacteria:   EBMYCT00000072004
- GeneID:   886714
- GeneID:   924523
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1449
- KEGG:   mtu:Rv1405c
- TIGR:   MT1449
- TubercuList:   Rv1405c
- GeneTree:   EBGT00050000014588
- HOGENOM:   HBG657462
- OMA:   IEAYANI
- ProtClustDB:   CLSK791141
- InterPro:   IPR013216

Pfam domain/function: PF08241 Methyltransf_11

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29263; Mature: 29131

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEECCC
GNISLPAAKTGATVISTDLTPELLQRSQARAAQQGLTLQYQEANAQALPFADDEFDTVIS
CCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHH
AIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGRMLATIRPYRPSVSADLPPSA
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHH
LWGREAYVTGLLGDGVTGLKTARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAV
HCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
LAAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR
EHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
>Mature Secondary Structure 
TIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEECCC
GNISLPAAKTGATVISTDLTPELLQRSQARAAQQGLTLQYQEANAQALPFADDEFDTVIS
CCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHH
AIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGRMLATIRPYRPSVSADLPPSA
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHH
LWGREAYVTGLLGDGVTGLKTARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAV
HCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
LAAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR
EHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036