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The map label for this gene is metG [H]

Identifier: 148660789

GI number: 148660789

Start: 1126752

End: 1128311

Strand: Reverse

Name: metG [H]

Synonym: MRA_1016

Alternate gene names: 148660789

Gene position: 1128311-1126752 (Counterclockwise)

Preceding gene: 148660796

Following gene: 148660787

Centisome position: 25.53

GC content: 64.1

Gene sequence:

>1560_bases
ATGAAGCCCTATTACGTCACCACCGCGATCGCATATCCCAACGCTGCACCCCACGTAGGTCACGCCTACGAATACATCGC
CACCGACGCGATCGCCCGGTTCAAACGGCTGGATCGCTATGACGTGCGCTTCCTGACCGGGACCGACGAGCATGGCCTGA
AGGTCGCACAAGCCGCCGCGGCAGCGGGCGTGCCCACCGCGGCGCTTGCCCGGCGCAATTCCGACGTGTTTCAGCGCATG
CAGGAGGCGCTGAACATCTCCTTCGACCGATTCATCCGCACTACCGATGCCGACCACCACGAGGCGTCCAAGGAACTCTG
GCGACGGATGTCGGCGGCCGGCGACATCTATCTGGACAACTATTCCGGGTGGTACTCGGTGCGCGACGAGCGGTTCTTCG
TCGAATCGGAGACCCAACTTGTCGACGGCACGCGCCTGACGGTAGAGACCGGCACGCCGGTGACCTGGACCGAGGAGCAG
ACCTACTTCTTCCGGCTGTCGGCCTATACCGACAAGCTGCTGGCCCACTATCACGCCAACCCCGACTTCATCGCGCCGGA
GACGCGGCGCAACGAAGTGATCAGCTTCGTCTCCGGCGGCCTGGACGACCTGTCGATCTCGCGCACCTCGTTTGACTGGG
GTGTGCAGGTGCCCGAGCACCCCGACCACGTCATGTACGTCTGGGTCGACGCGCTGACCAATTACCTGACCGGGGCGGGC
TTCCCGGATACCGACTCGGAGTTGTTCCGCCGCTACTGGCCCGCCGATTTGCACATGATCGGCAAGGACATCATCAGGTT
TCATGCCGTCTATTGGCCGGCGTTTTTGATGTCAGCCGGAATCGAGTTGCCGCGAAGGATCTTCGCGCACGGGTTCTTGC
ACAACCGCGGCGAGAAGATGAGTAAATCGGTGGGCAACATCGTCGACCCGGTTGCCCTGGCGGAAGCGCTCGGGGTGGAC
CAGGTCCGCTACTTCCTGTTGCGGGAGGTCCCGTTCGGCCAGGACGGCAGTTACAGCGACGAGGCCATCGTCACTCGGAT
CAACACCGATCTGGCCAACGAGCTCGGCAACTTGGCCCAACGCTCGTTGTCGATGGTGGCCAAAAACCTTGACGGCAGGG
TGCCCAACCCGGGTGAGTTCGCCGACGCCGACGCCGCGCTGCTTGCGACCGCCGATGGCTTGTTGGAGCGAGTGCGCGGT
CACTTCGACGCACAGGCGATGCACCTGGCGCTGGAGGCGATCTGGCTGATGCTCGGCGACGCGAACAAGTACTTTTCGGT
GCAGCAGCCGTGGGTACTGCGCAAGAGCGAGTCCGAAGCCGATCAGGCCCGGTTCCGCACCACGCTCTACGTCACCTGCG
AGGTAGTCCGCATCGCGGCACTGCTGATCCAGCCGGTGATGCCGGAGTCGGCCGGCAAAATTTTGGACCTGCTCGGCCAG
GCCCCAAACCAGCGGTCGTTCGCCGCCGTAGGTGTTCGGCTGACCCCCGGCACAGCGCTGCCGCCGCCCACCGGGGTATT
TCCCCGCTACCAGCCGCCGCAACCACCCGAAGGCAAGTGA

Upstream 100 bases:

>100_bases
GTGGGCGTCGACCAACGGCGCCAGGGGTTCCGGAGCGGGCGGTGGATCTCGTCTGGCTGGGCGGTCGGAGCTCACCCGCA
CACAATAGGGTGGTGTCGAA

Downstream 100 bases:

>100_bases
GCGGACCGCAGCGACGGGAAAGCCACCTACGAAGCGTTGACCGCGGTCTGCGCGTCGCGTGGGATGTCGAGCGTGGCGAC
GGGATAAAACCCGGAATCGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDRYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK

Sequences:

>Translated_519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDRYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
>Mature_519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDRYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=546, Percent_Identity=37.9120879120879, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI14043022, Length=536, Percent_Identity=26.3059701492537, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1788432, Length=502, Percent_Identity=25.8964143426295, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI212645096, Length=518, Percent_Identity=35.7142857142857, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI71992781, Length=412, Percent_Identity=34.2233009708738, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=25.1798561151079, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=33.9449541284404, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321704, Length=527, Percent_Identity=22.3908918406072, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=38.3877159309021, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=25.4025044722719, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58093; Mature: 58093

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDRYDVRFLTGTDEHGLKVAQAAA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AAGVPTAALARRNSDVFQRMQEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDN
HCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
YSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQTYFFRLSAYTDKLLAHYHAN
CCCEEEECCCEEEEECCCEEECCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCC
PDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
CCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCC
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
SKSVGNIVDPVALAEALGVDQVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
RSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRGHFDAQAMHLALEAIWLMLGD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
CCCCEECCCCEEEECCCCHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDRYDVRFLTGTDEHGLKVAQAAA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AAGVPTAALARRNSDVFQRMQEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDN
HCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
YSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQTYFFRLSAYTDKLLAHYHAN
CCCEEEECCCEEEEECCCEEECCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCC
PDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
CCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCC
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
SKSVGNIVDPVALAEALGVDQVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
RSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRGHFDAQAMHLALEAIWLMLGD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
CCCCEECCCCEEEECCCCHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]