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The map label for this gene is dus

Identifier: 148660601

GI number: 148660601

Start: 917783

End: 918952

Strand: Reverse

Name: dus

Synonym: MRA_0833

Alternate gene names: 148660601

Gene position: 918952-917783 (Counterclockwise)

Preceding gene: 148660602

Following gene: 148660600

Centisome position: 20.79

GC content: 67.18

Gene sequence:

>1170_bases
ATGAGCAGGAGGCGGGCAATCCAACCCAGCCCGGCGTTGCGCATCGGCCCGATCGAGCTCGCCAGCCCGGTTGTGCTGGC
TCCGATGGCCGGTGTGACGAACGTCGCATTCCGGGCGCTGTGTCGTCAGCTGGAACAGTCGAAGGTCGGCACGGTCAGCG
GACTGTATGTCTGCGAGATGGTGACCGCACGTGCGCTCATCGAGCGGCACCCGGTCACCATGCACATGACAACGTTCTCC
GCGGACGAATCACCACGCTCGCTGCAGCTGTACACCGTCGATCCGGACACCACGTATGCGGCCGCTCGGATGATCGCTGG
CGAAGGGTTAGCCGATCACATCGACATGAATTTCGGCTGCCCAGTGCCCAAAGTCACCAAGCGCGGCGGCGGGGCGGCGC
TACCGTTCAAACGGCGGCTATTCGGTCAGATCGTGGCCGCGGCTGTGCGCGCCACCGAAGGCACCGATATACCGGTGACG
GTCAAGTTTCGCATTGGCATCGATGACGCCCACCACACACATCTGGATGCCGGCCGCATCGCTGAGGCCGAAGGAGCCGC
TGCGGTCGCGCTGCACGCCCGTACCGCGGCACAACGCTATTCCGGCACCGCCGACTGGGAACAGATCGCCCGGCTCAAGC
AGCATGTCCGGACGATTCCGGTGCTCGGCAACGGCGACATCTACGATGCCGGCGACGCACTGGCCATGATGTCCACCACC
GGCTGTGACGGCGTGGTCATCGGCCGTGGCTGCCTGGGCCGTCCCTGGCTATTTGCCGAGTTGTCCGCGGCTTTCACCGG
CAGCCCGGCCCCCACCCCACCCACGCTCGGCGAAGTCGCCGACATCATCCGCCGTCACGGCACCTTGCTGGCCGCACACT
TCGGCGAGGACAAGGGCATGCGCGATATCCGCAAGCACATCGCCTGGTATCTCCATGGCTTCCCGGCGGGCTCCGCGCTC
CGGCGGGCGTTAGCAATGGTCAAGACGTTCGACGAGCTCGACTGCCTGCTGGACCGGTTGGACGGCACGGTACCGTTCCC
GGACTCCGCGACTGGCGCCCGGGGCCGGCAGGGTTCCCCCGCCCGGGTGGCCCTTCCCGACGGCTGGCTAACCGACCCCG
ACGACTGCCGAGTGCCGGAAGGGGCGGACGCCATGGGGTCGGGTGGCTGA

Upstream 100 bases:

>100_bases
GAGCCGTCGTTAGCCCGGCGACGATGCAGAGCGCGCAGCGCGATGAGCAGGAGGCGGGCAATCCAACCCAGCCCGGCGAC
GATGCAGAGCGCGCAGCGCG

Downstream 100 bases:

>100_bases
ATCGAGACCCTGTCGAAATCGCGTCTGGACCGATAAATCAGTACGATGTGGGCCTTGCTGCATTGTGCGGCGCCGACTCG
TGGCGGTGCTGCGCGAAGGT

Product: NifR3/SMM1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFS
ADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVT
VKFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSAL
RRALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG

Sequences:

>Translated_389_residues
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFS
ADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVT
VKFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSAL
RRALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG
>Mature_388_residues
SRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFSA
DESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVTV
KFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTTG
CDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSALR
RALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Homo sapiens, GI239788483, Length=306, Percent_Identity=30.3921568627451, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI239788462, Length=302, Percent_Identity=30.4635761589404, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=30.6382978723404, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI40807366, Length=318, Percent_Identity=27.6729559748428, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=32.8125, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=31.1965811965812, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=301, Percent_Identity=26.578073089701, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=233, Percent_Identity=30.9012875536481, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=323, Percent_Identity=23.8390092879257, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=240, Percent_Identity=26.25, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=293, Percent_Identity=27.6450511945392, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921524, Length=238, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS_MYCTU (O53835)

Other databases:

- EMBL:   BX842574
- EMBL:   AE000516
- PIR:   G70810
- RefSeq:   NP_215338.1
- RefSeq:   NP_335273.1
- ProteinModelPortal:   O53835
- SMR:   O53835
- EnsemblBacteria:   EBMYCT00000002102
- EnsemblBacteria:   EBMYCT00000070313
- GeneID:   885380
- GeneID:   926148
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0845
- KEGG:   mtu:Rv0823c
- TIGR:   MT0845
- TubercuList:   Rv0823c
- GeneTree:   EBGT00050000017379
- HOGENOM:   HBG557545
- OMA:   GIDDDHL
- ProtClustDB:   CLSK871859
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 41389; Mature: 41258

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEM
CCCCCCCCCCCCEEECCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VTARALIERHPVTMHMTTFSADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGC
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCC
PVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVTVKFRIGIDDAHHTHLDAGRI
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
AEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
CCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECC
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGM
CCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
RDIRKHIAWYLHGFPAGSALRRALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARVALPDGWLTDPDDCRVPEGADAMGSGG
CEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEM
CCCCCCCCCCCEEECCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VTARALIERHPVTMHMTTFSADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGC
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCC
PVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVTVKFRIGIDDAHHTHLDAGRI
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
AEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
CCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECC
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGM
CCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
RDIRKHIAWYLHGFPAGSALRRALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARVALPDGWLTDPDDCRVPEGADAMGSGG
CEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036