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The map label for this gene is tam

Identifier: 148660060

GI number: 148660060

Start: 359533

End: 360318

Strand: Direct

Name: tam

Synonym: MRA_0303

Alternate gene names: 148660060

Gene position: 359533-360318 (Clockwise)

Preceding gene: 148660058

Following gene: 148660063

Centisome position: 8.13

GC content: 65.9

Gene sequence:

>786_bases
ATGTGGGATCCCGACGTCTACCTGGCTTTTTCGGGTCATCGCAACCGCCCGTTCTACGAGTTGGTGTCACGGGTGGGTCT
CGAGCGGGCGCGCCGCGTGGTCGACCTGGGGTGCGGGCCCGGCCACCTGACACGCTACCTGGCACGACGATGGCCCGGCG
CGGTGATCGAGGCTCTGGACAGCTCACCGGAGATGGTCGCTGCCGCGGCCGAACGCGGGATCGACGCCACCACCGGTGAC
CTGCGGGACTGGAAACCAAAGCCCGACACCGATGTGGTGGTGAGCAACGCTGCGTTGCATTGGGTGCCTGAGCATTCCGA
CCTGTTGGTCCGGTGGGTCGACGAGCTGGCGCCGGGATCATGGATCGCTGTTCAGATCCCCGGCAACTTCGAGACGCCGT
CGCACGCCGCGGTACGGGCGTTGGCCCGCCGCGAGCCGTATGCAAAGCTAATGCGCGACATACCTTTTCGTGTGGGCGCG
GTGGTCCAATCTCCGGCGTATTACGCGGAGCTGCTGATGGACACCGGCTGCAAGGTCGACGTGTGGGAGACCACGTACCT
ACACCAGCTGACCGGCGAGCACCCGGTGTTGGACTGGATTACCGGAAGCGCGCTGGTCCCAGTGCGTGAGCGGCTCAGCG
ATGAGAGCTGGCAGCAGTTTCGGCAGGAGCTCATTCCGCTGCTGAACGACGCCTACCCGCCACGGGCCGACGGTAGCACC
ATCTTTCCCTTCCGGCGGCTGTTCATGGTCGCCGAAGTTGGTGGCGCGCGCCGCTCAGGTGGGTAG

Upstream 100 bases:

>100_bases
CATCAATGTCGATGGTAATCGCCAATGTGGCTGACCGCTGAAGGTTTCGACTGTATCGTCAATTTCTCACTCGGTCGAGC
GCTTGTCCAGGAGCACGTAC

Downstream 100 bases:

>100_bases
CCCCAGCCGCGGCGCCTCCGCTCGGTACCGGTCGACCCACTCATCAGAGCGCTGGTTGGCCTGCCGTTCCAGCATCGGTG
CGGGCGCCAGTTTGGGATCC

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MWDPDVYLAFSGHRNRPFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALDSSPEMVAAAAERGIDATTGD
LRDWKPKPDTDVVVSNAALHWVPEHSDLLVRWVDELAPGSWIAVQIPGNFETPSHAAVRALARREPYAKLMRDIPFRVGA
VVQSPAYYAELLMDTGCKVDVWETTYLHQLTGEHPVLDWITGSALVPVRERLSDESWQQFRQELIPLLNDAYPPRADGST
IFPFRRLFMVAEVGGARRSGG

Sequences:

>Translated_261_residues
MWDPDVYLAFSGHRNRPFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALDSSPEMVAAAAERGIDATTGD
LRDWKPKPDTDVVVSNAALHWVPEHSDLLVRWVDELAPGSWIAVQIPGNFETPSHAAVRALARREPYAKLMRDIPFRVGA
VVQSPAYYAELLMDTGCKVDVWETTYLHQLTGEHPVLDWITGSALVPVRERLSDESWQQFRQELIPLLNDAYPPRADGST
IFPFRRLFMVAEVGGARRSGG
>Mature_261_residues
MWDPDVYLAFSGHRNRPFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALDSSPEMVAAAAERGIDATTGD
LRDWKPKPDTDVVVSNAALHWVPEHSDLLVRWVDELAPGSWIAVQIPGNFETPSHAAVRALARREPYAKLMRDIPFRVGA
VVQSPAYYAELLMDTGCKVDVWETTYLHQLTGEHPVLDWITGSALVPVRERLSDESWQQFRQELIPLLNDAYPPRADGST
IFPFRRLFMVAEVGGARRSGG

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family

Homologues:

Organism=Escherichia coli, GI1787798, Length=252, Percent_Identity=38.0952380952381, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAM_MYCBO (P66886)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_853966.1
- ProteinModelPortal:   P66886
- SMR:   P66886
- EnsemblBacteria:   EBMYCT00000016475
- GeneID:   1091133
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0302
- GeneTree:   EBGT00050000017741
- HOGENOM:   HBG328684
- OMA:   GRTLFPF
- ProtClustDB:   PRK14103
- BioCyc:   MBOV233413:MB0302-MONOMER
- BRENDA:   2.1.1.144
- GO:   GO:0005737
- HAMAP:   MF_00560
- InterPro:   IPR013216
- InterPro:   IPR023149

Pfam domain/function: PF08241 Methyltransf_11

EC number: =2.1.1.144

Molecular weight: Translated: 29269; Mature: 29269

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDPDVYLAFSGHRNRPFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALD
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHC
SSPEMVAAAAERGIDATTGDLRDWKPKPDTDVVVSNAALHWVPEHSDLLVRWVDELAPGS
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHHCCCC
WIAVQIPGNFETPSHAAVRALARREPYAKLMRDIPFRVGAVVQSPAYYAELLMDTGCKVD
EEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCEEE
VWETTYLHQLTGEHPVLDWITGSALVPVRERLSDESWQQFRQELIPLLNDAYPPRADGST
EEHHHHHHHHCCCCCEEHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
IFPFRRLFMVAEVGGARRSGG
EHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MWDPDVYLAFSGHRNRPFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALD
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHC
SSPEMVAAAAERGIDATTGDLRDWKPKPDTDVVVSNAALHWVPEHSDLLVRWVDELAPGS
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHHCCCC
WIAVQIPGNFETPSHAAVRALARREPYAKLMRDIPFRVGAVVQSPAYYAELLMDTGCKVD
EEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCEEE
VWETTYLHQLTGEHPVLDWITGSALVPVRERLSDESWQQFRQELIPLLNDAYPPRADGST
EEHHHHHHHHCCCCCEEHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
IFPFRRLFMVAEVGGARRSGG
EHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972