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The map label for this gene is dfrA [H]

Identifier: 148654129

GI number: 148654129

Start: 2900030

End: 2900611

Strand: Direct

Name: dfrA [H]

Synonym: PsycPRwf_2332

Alternate gene names: 148654129

Gene position: 2900030-2900611 (Clockwise)

Preceding gene: 148654128

Following gene: 148654130

Centisome position: 97.35

GC content: 45.53

Gene sequence:

>582_bases
ATGAGCTACGCCCACACTCAAGTTGCCCAAATTGTGGCCATTAGCCGCAACCGCTGTATCGGCAAGGGCAATGATTTGCC
TTGGCATATCCCCAATGATTTAAAACACTTTAAGGCCATGACCACCGCTGAGACCGATGCTTATGTAGATACAGGCCTAA
AAGGTATTGTCATTATGGGCCGTAAAACTTTTGAATCGATGGGCTCAAAGCCGCTGCCAAAACGAGTGAACTTCATTGTC
ACCACTCAAATGGACTATGCGCAGCAAAAAGGGCTAGAAGGTCTTAAAGATGTGCATGTGGTGCATAACTTGGATGATGC
TTTAACCCATGCAGCCAACGTCGCTCATGGCTTAAAGTTTGAAACTATTTGGGTTATCGGCGGCGAGCGTGTGTTTAAAC
GTGCCATGATGTTCACCGACCGCATTGAGCTAACGGTTGTCGATACCGACATTGAAGACGGCGATGCATTTTACCCAGAG
ATACCCGAAGACTTTGAACTCACTCAAGAATCTGAGTCATTTACTGATGAAAAAAGCGGCCTGAGCTATAAGTTTATGAC
TTATATGGTCAAAAAGGGCTAA

Upstream 100 bases:

>100_bases
CGCGGCATCAATAGATAGTTACAGCATAAATAGATAGCTTAGCCGCTATACGCCAAGCCGTTTATCAGACAATTGGTTAT
TATTGATTAAGGATATTTGT

Downstream 100 bases:

>100_bases
CCCTTGTTTAGTTGTTTGCTTCTAAAGACAGCTATTGTCTGAAGCCCGCTATGCTCTAGCGGGCTTTTTTGTGACTACCC
TTTTGCTATTATGACTCAAG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSYAHTQVAQIVAISRNRCIGKGNDLPWHIPNDLKHFKAMTTAETDAYVDTGLKGIVIMGRKTFESMGSKPLPKRVNFIV
TTQMDYAQQKGLEGLKDVHVVHNLDDALTHAANVAHGLKFETIWVIGGERVFKRAMMFTDRIELTVVDTDIEDGDAFYPE
IPEDFELTQESESFTDEKSGLSYKFMTYMVKKG

Sequences:

>Translated_193_residues
MSYAHTQVAQIVAISRNRCIGKGNDLPWHIPNDLKHFKAMTTAETDAYVDTGLKGIVIMGRKTFESMGSKPLPKRVNFIV
TTQMDYAQQKGLEGLKDVHVVHNLDDALTHAANVAHGLKFETIWVIGGERVFKRAMMFTDRIELTVVDTDIEDGDAFYPE
IPEDFELTQESESFTDEKSGLSYKFMTYMVKKG
>Mature_192_residues
SYAHTQVAQIVAISRNRCIGKGNDLPWHIPNDLKHFKAMTTAETDAYVDTGLKGIVIMGRKTFESMGSKPLPKRVNFIVT
TQMDYAQQKGLEGLKDVHVVHNLDDALTHAANVAHGLKFETIWVIGGERVFKRAMMFTDRIELTVVDTDIEDGDAFYPEI
PEDFELTQESESFTDEKSGLSYKFMTYMVKKG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=188, Percent_Identity=34.0425531914894, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI28827793, Length=184, Percent_Identity=33.1521739130435, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1786233, Length=180, Percent_Identity=32.7777777777778, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17505899, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=182, Percent_Identity=31.8681318681319, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24647458, Length=182, Percent_Identity=31.8681318681319, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21782; Mature: 21651

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAHTQVAQIVAISRNRCIGKGNDLPWHIPNDLKHFKAMTTAETDAYVDTGLKGIVIMG
CCCCHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCHHHHCCCCEEEEEC
RKTFESMGSKPLPKRVNFIVTTQMDYAQQKGLEGLKDVHVVHNLDDALTHAANVAHGLKF
CHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
ETIWVIGGERVFKRAMMFTDRIELTVVDTDIEDGDAFYPEIPEDFELTQESESFTDEKSG
EEEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCC
LSYKFMTYMVKKG
CCEEEEEEEEECC
>Mature Secondary Structure 
SYAHTQVAQIVAISRNRCIGKGNDLPWHIPNDLKHFKAMTTAETDAYVDTGLKGIVIMG
CCCHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCHHHHCCCCEEEEEC
RKTFESMGSKPLPKRVNFIVTTQMDYAQQKGLEGLKDVHVVHNLDDALTHAANVAHGLKF
CHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
ETIWVIGGERVFKRAMMFTDRIELTVVDTDIEDGDAFYPEIPEDFELTQESESFTDEKSG
EEEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCC
LSYKFMTYMVKKG
CCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]