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The map label for this gene is thyA

Identifier: 148654128

GI number: 148654128

Start: 2899060

End: 2899920

Strand: Direct

Name: thyA

Synonym: PsycPRwf_2331

Alternate gene names: 148654128

Gene position: 2899060-2899920 (Clockwise)

Preceding gene: 148654127

Following gene: 148654129

Centisome position: 97.32

GC content: 45.99

Gene sequence:

>861_bases
ATGATAACTTCCAAAAATGAAGCCGCTTATCTTGATTTAATTCGCTATGTGCGTGACCACGGTACCGAGAAGGGAGACCG
TACAGGTACGGGCACCCGCAGTCATTTTGGCGCTCAGTTGCGCTTTGATTTAAAAGATGGCTTTCCGCTGTTAACGACCA
AAAAAGTGCACATGAAGTCGATTACTTATGAACTGTTTTGGTTTTTAAAAGGCGATACCCATGTCAAATATTTGCAAGAC
CATGGGGTACGTATTTGGAACGAATGGTCAACTGCGGAGCAAACCGCTCGCTTTGGTCGCCCTGAGGGTGAGCTGGGTCC
TATCTATGGTCATCAGTGGCGTAATTATGGGGCCACTAAAGCGGAGGACGGCCGCTACAATCAAGATGGGGTGGACCAAG
TTGTTGAGGTAATTGAGCAAATTAAGAGCAACCCCAATTCGCGTCGTTTGATTGTGTCCGGTTGGAACCCAGCCGAGGCG
ACCCAAGTGGCGCTACCGCCTTGCCATACATTATTTCAGTTCTTTGTGGCAGACGGCAAACTATCTTGCCAGCTGTATCA
GCGCTCCGCAGATTTGTTTTTAGGGGTGCCTTTTAACATTGCCAGCTACTCTTTATTAACACATATGATTGCTCAAGTTT
GTGACCTAGAGGTGGGTGAGTTTATTTGGACAGGTGGTGATTGTCACTTGTATCAAAACCATATTGATCAGGTCAATGAA
CAGCTGTCTCGCGAAGCTTATGAGTTACCTAAATTATGGCTCAACCCTGAAATCAAGGATATTTTTGATTTTACTTTTGA
TGATATCCGAGTTGAGGGCTATCAGTCGCATCCAGCGATTAAAGCACCCGTCGCGGTATAA

Upstream 100 bases:

>100_bases
GCAATATGACTGGGGCAAATACGCCGCTTAGAAATAATAGATTTTTGCTGTATTAACTGTTTTAGCTCACTTACTTTGTT
AATTATTTGACTGTAGACCT

Downstream 100 bases:

>100_bases
ATAGCCCGTCGCGGCATCAATAGATAGTTACAGCATAAATAGATAGCTTAGCCGCTATACGCCAAGCCGTTTATCAGACA
ATTGGTTATTATTGATTAAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MITSKNEAAYLDLIRYVRDHGTEKGDRTGTGTRSHFGAQLRFDLKDGFPLLTTKKVHMKSITYELFWFLKGDTHVKYLQD
HGVRIWNEWSTAEQTARFGRPEGELGPIYGHQWRNYGATKAEDGRYNQDGVDQVVEVIEQIKSNPNSRRLIVSGWNPAEA
TQVALPPCHTLFQFFVADGKLSCQLYQRSADLFLGVPFNIASYSLLTHMIAQVCDLEVGEFIWTGGDCHLYQNHIDQVNE
QLSREAYELPKLWLNPEIKDIFDFTFDDIRVEGYQSHPAIKAPVAV

Sequences:

>Translated_286_residues
MITSKNEAAYLDLIRYVRDHGTEKGDRTGTGTRSHFGAQLRFDLKDGFPLLTTKKVHMKSITYELFWFLKGDTHVKYLQD
HGVRIWNEWSTAEQTARFGRPEGELGPIYGHQWRNYGATKAEDGRYNQDGVDQVVEVIEQIKSNPNSRRLIVSGWNPAEA
TQVALPPCHTLFQFFVADGKLSCQLYQRSADLFLGVPFNIASYSLLTHMIAQVCDLEVGEFIWTGGDCHLYQNHIDQVNE
QLSREAYELPKLWLNPEIKDIFDFTFDDIRVEGYQSHPAIKAPVAV
>Mature_286_residues
MITSKNEAAYLDLIRYVRDHGTEKGDRTGTGTRSHFGAQLRFDLKDGFPLLTTKKVHMKSITYELFWFLKGDTHVKYLQD
HGVRIWNEWSTAEQTARFGRPEGELGPIYGHQWRNYGATKAEDGRYNQDGVDQVVEVIEQIKSNPNSRRLIVSGWNPAEA
TQVALPPCHTLFQFFVADGKLSCQLYQRSADLFLGVPFNIASYSLLTHMIAQVCDLEVGEFIWTGGDCHLYQNHIDQVNE
QLSREAYELPKLWLNPEIKDIFDFTFDDIRVEGYQSHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=284, Percent_Identity=49.6478873239437, Blast_Score=283, Evalue=9e-77,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=57.4007220216607, Blast_Score=339, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=48.2638888888889, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324648, Length=296, Percent_Identity=39.8648648648649, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137556, Length=290, Percent_Identity=48.2758620689655, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_PSYWF (A5WHY0)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001281221.1
- ProteinModelPortal:   A5WHY0
- SMR:   A5WHY0
- STRING:   A5WHY0
- GeneID:   5205646
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_2331
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   PSP56811:PSYCPRWF_2331-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32730; Mature: 32730

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSKNEAAYLDLIRYVRDHGTEKGDRTGTGTRSHFGAQLRFDLKDGFPLLTTKKVHMKS
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEEEEECCCCCCEEEECHHHHHH
ITYELFWFLKGDTHVKYLQDHGVRIWNEWSTAEQTARFGRPEGELGPIYGHQWRNYGATK
HEEEEEEEEECCCEEEEHHHCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AEDGRYNQDGVDQVVEVIEQIKSNPNSRRLIVSGWNPAEATQVALPPCHTLFQFFVADGK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHCCCC
LSCQLYQRSADLFLGVPFNIASYSLLTHMIAQVCDLEVGEFIWTGGDCHLYQNHIDQVNE
EEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHHHH
QLSREAYELPKLWLNPEIKDIFDFTFDDIRVEGYQSHPAIKAPVAV
HHHHHHHHCCHHHCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MITSKNEAAYLDLIRYVRDHGTEKGDRTGTGTRSHFGAQLRFDLKDGFPLLTTKKVHMKS
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEEEEECCCCCCEEEECHHHHHH
ITYELFWFLKGDTHVKYLQDHGVRIWNEWSTAEQTARFGRPEGELGPIYGHQWRNYGATK
HEEEEEEEEECCCEEEEHHHCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AEDGRYNQDGVDQVVEVIEQIKSNPNSRRLIVSGWNPAEATQVALPPCHTLFQFFVADGK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHCCCC
LSCQLYQRSADLFLGVPFNIASYSLLTHMIAQVCDLEVGEFIWTGGDCHLYQNHIDQVNE
EEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHHHH
QLSREAYELPKLWLNPEIKDIFDFTFDDIRVEGYQSHPAIKAPVAV
HHHHHHHHCCHHHCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA