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The map label for this gene is truA [H]

Identifier: 148653728

GI number: 148653728

Start: 2397325

End: 2398233

Strand: Direct

Name: truA [H]

Synonym: PsycPRwf_1931

Alternate gene names: 148653728

Gene position: 2397325-2398233 (Clockwise)

Preceding gene: 148653727

Following gene: 148653729

Centisome position: 80.47

GC content: 53.14

Gene sequence:

>909_bases
ATGACGTTTACCCCCTCAGATCGAAGCGATACACAACTACCCACTGCCTCAAGTGATTTGCTTGAGACGACCACAGCACC
CTCTAGCAGCACCCCTCAGCACCCTGCTCAAACCTATGCGCTAGGTGTTGAATTCAATGGCGCAAACTATCGGGGATGGC
AGCGCCAGCAAGAGGTGTTGTGTGTACAGCAGGTACTCGAGGAGACACTGAGCAAAATTGCCAATGAGCCCATTGAAGTG
GTGGCAGCCGGTCGTACAGATGCTGGCGTGCATGGCGGTAATATGCTGGCTCACTTCACCACTTCTGCCAAGCGCGGCGT
ATATAATTGGCTGCGCGGTGCCAACAGTCTGCTGCCTGATGACATCGCCATTCGCTGGATTCAGCCCATGCCCGATACGT
TTCATGCCCGCTTTGGCGCCATCGCCCGCCGTTACCGCTATATTACTCTCAATCAGCCGCAGCGCCCGGCGGTCCTCAAT
CACTTGGTGACCCACATTCATGAGCCCTTAGATTTGGCATTGATGCAGCAGGCCGCTGAGCATATCGTCGGCACCCATGA
CTTTAGCAGCTTTCGCGCCGCTGCCTGCCAATCTAAGCAGCCGGTACGCAATATTCATCATGCTAAGCTATTTAAGCATG
GCGAGTTTATTGTGTTTGATATTCAGGCAGATGGCTTCTTGCATCACATGGTACGAAACCTGATGGGCACCTTGTTTGCC
ATTGGCAAAGGTGAGCTTCAACCTGAGGATTACTTACGGATTTTGCAGGCCAAAGACCGTACCATTGCCCCGCCGACAGC
GCCGCCAGATGGGCTATATTTTATTAACGCCTATTACCCTGAGCCAATGCAAGCCCTACTACCAAAGGTGCCACTAGCAC
CGCAGTGGCTAAATCTGCCCGAACAATAA

Upstream 100 bases:

>100_bases
CAGCGCTGGCAATACAGCGTAAATAAGGCATAAACTTTGCCTAAAAATCTTTATAATGCCCCCTTTTTATCCTATTTTAC
CGACCAATTTGGCTTTACCC

Downstream 100 bases:

>100_bases
GCGCTCTAACCATCCATTAACATGCGAACCATCCATTAATAAGCAACTAGCAAATTCGACGTATTTTATCAAGTGATACC
ACAACAAAATTTGCTTAATC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTFTPSDRSDTQLPTASSDLLETTTAPSSSTPQHPAQTYALGVEFNGANYRGWQRQQEVLCVQQVLEETLSKIANEPIEV
VAAGRTDAGVHGGNMLAHFTTSAKRGVYNWLRGANSLLPDDIAIRWIQPMPDTFHARFGAIARRYRYITLNQPQRPAVLN
HLVTHIHEPLDLALMQQAAEHIVGTHDFSSFRAAACQSKQPVRNIHHAKLFKHGEFIVFDIQADGFLHHMVRNLMGTLFA
IGKGELQPEDYLRILQAKDRTIAPPTAPPDGLYFINAYYPEPMQALLPKVPLAPQWLNLPEQ

Sequences:

>Translated_302_residues
MTFTPSDRSDTQLPTASSDLLETTTAPSSSTPQHPAQTYALGVEFNGANYRGWQRQQEVLCVQQVLEETLSKIANEPIEV
VAAGRTDAGVHGGNMLAHFTTSAKRGVYNWLRGANSLLPDDIAIRWIQPMPDTFHARFGAIARRYRYITLNQPQRPAVLN
HLVTHIHEPLDLALMQQAAEHIVGTHDFSSFRAAACQSKQPVRNIHHAKLFKHGEFIVFDIQADGFLHHMVRNLMGTLFA
IGKGELQPEDYLRILQAKDRTIAPPTAPPDGLYFINAYYPEPMQALLPKVPLAPQWLNLPEQ
>Mature_301_residues
TFTPSDRSDTQLPTASSDLLETTTAPSSSTPQHPAQTYALGVEFNGANYRGWQRQQEVLCVQQVLEETLSKIANEPIEVV
AAGRTDAGVHGGNMLAHFTTSAKRGVYNWLRGANSLLPDDIAIRWIQPMPDTFHARFGAIARRYRYITLNQPQRPAVLNH
LVTHIHEPLDLALMQQAAEHIVGTHDFSSFRAAACQSKQPVRNIHHAKLFKHGEFIVFDIQADGFLHHMVRNLMGTLFAI
GKGELQPEDYLRILQAKDRTIAPPTAPPDGLYFINAYYPEPMQALLPKVPLAPQWLNLPEQ

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=286, Percent_Identity=30.0699300699301, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788657, Length=270, Percent_Identity=50.7407407407407, Blast_Score=280, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6321112, Length=252, Percent_Identity=29.7619047619048, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33771; Mature: 33640

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTPSDRSDTQLPTASSDLLETTTAPSSSTPQHPAQTYALGVEFNGANYRGWQRQQEVL
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
CVQQVLEETLSKIANEPIEVVAAGRTDAGVHGGNMLAHFTTSAKRGVYNWLRGANSLLPD
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
DIAIRWIQPMPDTFHARFGAIARRYRYITLNQPQRPAVLNHLVTHIHEPLDLALMQQAAE
HHEEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
HIVGTHDFSSFRAAACQSKQPVRNIHHAKLFKHGEFIVFDIQADGFLHHMVRNLMGTLFA
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHH
IGKGELQPEDYLRILQAKDRTIAPPTAPPDGLYFINAYYPEPMQALLPKVPLAPQWLNLP
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCCCHHHCCCC
EQ
CC
>Mature Secondary Structure 
TFTPSDRSDTQLPTASSDLLETTTAPSSSTPQHPAQTYALGVEFNGANYRGWQRQQEVL
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
CVQQVLEETLSKIANEPIEVVAAGRTDAGVHGGNMLAHFTTSAKRGVYNWLRGANSLLPD
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
DIAIRWIQPMPDTFHARFGAIARRYRYITLNQPQRPAVLNHLVTHIHEPLDLALMQQAAE
HHEEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
HIVGTHDFSSFRAAACQSKQPVRNIHHAKLFKHGEFIVFDIQADGFLHHMVRNLMGTLFA
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHH
IGKGELQPEDYLRILQAKDRTIAPPTAPPDGLYFINAYYPEPMQALLPKVPLAPQWLNLP
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCCCHHHCCCC
EQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA