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The map label for this gene is ansA [C]

Identifier: 148653727

GI number: 148653727

Start: 2396154

End: 2397257

Strand: Direct

Name: ansA [C]

Synonym: PsycPRwf_1930

Alternate gene names: 148653727

Gene position: 2396154-2397257 (Clockwise)

Preceding gene: 148653726

Following gene: 148653728

Centisome position: 80.44

GC content: 49.0

Gene sequence:

>1104_bases
ATGAGACAACGACCTACCATGACCGCACACATTAACACCACAGTCACCACCGACAACACCTCAGATCTGAATCAAGTCCA
ACTTATCTATGCCGGCGGTACTTTTGGTAGCTATGGCAAGCCGTTATCTGCACTGCCTGCTGACACCTTTTTGCCCCTAC
TTTTGGATAAAATATATCAAAACCTAACTTCAAAAATTGAGGTGTTGCCAAATACTTGTGTCAAAGACAGCAGTCAACTG
TCACCGGCTGATTTGGCACATTTTTTTGAGCTGATTTTGATGCGTTATGCGCAGGGGTATCGTAAGTTTGTACTGATTAC
TGGTACTGATACCCTTAGTTATCTGGGGTCATTTTTGGCAGAAGCTTTCGCCGGTAGCGATTTGTGCCTAGCATTGACCG
CCAGTATGCGCCCGCTATTTGATGCCAATGACATTCATAATTATCATATCGATGCACACAGTGATGCTTGGGGCAATTTA
TCAGAGGCCATCGGACTTGCCAGCCATGGCGAGCCTGGAGTTTCCATCACTTTTGCAGGTGAGTCTTGGCCGGCGCAAAC
CGTACAAAAAATCCATAGCCATGACCTCATGGCCTTCACCGGTCACCAACGCGCTGGCTATCCGGCCAACAGTTATCAAA
AAATACTGCCTGCCACACGCCGTCATAACTGGATTGGTGACTCTCAGCACAAGCTGCCTGCTGTGTTAGAGGCTGCTAAA
GACACCATCATTCCCACCCTCTACTGTGTCCCAAATAGCGCAGACTGGCTGGCCACTCAGTTAGATAACTTATTAAGCTT
GCCGCCGACTGGGGTGATTTTAATGGGCTTTGGTGCAGGCAATATCCCTTATAGCGATAAGCTTGCTGCCACTATGGATA
CGGTAGCAGCACACGGCCATATCCTCGTTTGTACGACACAGTGCCCTTATGGCGGAGTCAGTGAGGCTTATGCTGCTGGT
AGCTGGCAATATGAGCACCAAGTGTTATCGGCCGGCAGACTGACCATCCCTGCTGTGTATGCCCGACTGCTGTGGTTACA
TCTGGCTTATGACACACCCGCTAGACGTCGTCAGCGCTGGCAATACAGCGTAAATAAGGCATAA

Upstream 100 bases:

>100_bases
CAAATACTTGGCCAGCTTTACTGATCTAAATGTTAAGCCATTGGGTTTATACCACGTCTATACTGCATTTATACTATGTT
TATACCACTGCCATTAACTT

Downstream 100 bases:

>100_bases
ACTTTGCCTAAAAATCTTTATAATGCCCCCTTTTTATCCTATTTTACCGACCAATTTGGCTTTACCCATGACGTTTACCC
CCTCAGATCGAAGCGATACA

Product: asparaginase/glutaminase

Products: L-Aspartate; ammonia [C]

Alternate protein names: L-Asparaginase Type I; Asparaginase; L-Asparaginase I; Cytoplasmic Asparaginase I; Asparaginase/Glutaminase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRQRPTMTAHINTTVTTDNTSDLNQVQLIYAGGTFGSYGKPLSALPADTFLPLLLDKIYQNLTSKIEVLPNTCVKDSSQL
SPADLAHFFELILMRYAQGYRKFVLITGTDTLSYLGSFLAEAFAGSDLCLALTASMRPLFDANDIHNYHIDAHSDAWGNL
SEAIGLASHGEPGVSITFAGESWPAQTVQKIHSHDLMAFTGHQRAGYPANSYQKILPATRRHNWIGDSQHKLPAVLEAAK
DTIIPTLYCVPNSADWLATQLDNLLSLPPTGVILMGFGAGNIPYSDKLAATMDTVAAHGHILVCTTQCPYGGVSEAYAAG
SWQYEHQVLSAGRLTIPAVYARLLWLHLAYDTPARRRQRWQYSVNKA

Sequences:

>Translated_367_residues
MRQRPTMTAHINTTVTTDNTSDLNQVQLIYAGGTFGSYGKPLSALPADTFLPLLLDKIYQNLTSKIEVLPNTCVKDSSQL
SPADLAHFFELILMRYAQGYRKFVLITGTDTLSYLGSFLAEAFAGSDLCLALTASMRPLFDANDIHNYHIDAHSDAWGNL
SEAIGLASHGEPGVSITFAGESWPAQTVQKIHSHDLMAFTGHQRAGYPANSYQKILPATRRHNWIGDSQHKLPAVLEAAK
DTIIPTLYCVPNSADWLATQLDNLLSLPPTGVILMGFGAGNIPYSDKLAATMDTVAAHGHILVCTTQCPYGGVSEAYAAG
SWQYEHQVLSAGRLTIPAVYARLLWLHLAYDTPARRRQRWQYSVNKA
>Mature_367_residues
MRQRPTMTAHINTTVTTDNTSDLNQVQLIYAGGTFGSYGKPLSALPADTFLPLLLDKIYQNLTSKIEVLPNTCVKDSSQL
SPADLAHFFELILMRYAQGYRKFVLITGTDTLSYLGSFLAEAFAGSDLCLALTASMRPLFDANDIHNYHIDAHSDAWGNL
SEAIGLASHGEPGVSITFAGESWPAQTVQKIHSHDLMAFTGHQRAGYPANSYQKILPATRRHNWIGDSQHKLPAVLEAAK
DTIIPTLYCVPNSADWLATQLDNLLSLPPTGVILMGFGAGNIPYSDKLAATMDTVAAHGHILVCTTQCPYGGVSEAYAAG
SWQYEHQVLSAGRLTIPAVYARLLWLHLAYDTPARRRQRWQYSVNKA

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.1 [C]

Molecular weight: Translated: 40194; Mature: 40194

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRPTMTAHINTTVTTDNTSDLNQVQLIYAGGTFGSYGKPLSALPADTFLPLLLDKIYQ
CCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NLTSKIEVLPNTCVKDSSQLSPADLAHFFELILMRYAQGYRKFVLITGTDTLSYLGSFLA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHH
EAFAGSDLCLALTASMRPLFDANDIHNYHIDAHSDAWGNLSEAIGLASHGEPGVSITFAG
HHHCCCCEEEEEHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECC
ESWPAQTVQKIHSHDLMAFTGHQRAGYPANSYQKILPATRRHNWIGDSQHKLPAVLEAAK
CCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHH
DTIIPTLYCVPNSADWLATQLDNLLSLPPTGVILMGFGAGNIPYSDKLAATMDTVAAHGH
HHHCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCE
ILVCTTQCPYGGVSEAYAAGSWQYEHQVLSAGRLTIPAVYARLLWLHLAYDTPARRRQRW
EEEEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
QYSVNKA
CCCCCCC
>Mature Secondary Structure
MRQRPTMTAHINTTVTTDNTSDLNQVQLIYAGGTFGSYGKPLSALPADTFLPLLLDKIYQ
CCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NLTSKIEVLPNTCVKDSSQLSPADLAHFFELILMRYAQGYRKFVLITGTDTLSYLGSFLA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHH
EAFAGSDLCLALTASMRPLFDANDIHNYHIDAHSDAWGNLSEAIGLASHGEPGVSITFAG
HHHCCCCEEEEEHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECC
ESWPAQTVQKIHSHDLMAFTGHQRAGYPANSYQKILPATRRHNWIGDSQHKLPAVLEAAK
CCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHH
DTIIPTLYCVPNSADWLATQLDNLLSLPPTGVILMGFGAGNIPYSDKLAATMDTVAAHGH
HHHCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCE
ILVCTTQCPYGGVSEAYAAGSWQYEHQVLSAGRLTIPAVYARLLWLHLAYDTPARRRQRW
EEEEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
QYSVNKA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Asparagine; H2O [C]

Specific reaction: L-Asparagine + H2O --> L-Aspartate + ammonia [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA