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The map label for this gene is miaB

Identifier: 148653672

GI number: 148653672

Start: 2333189

End: 2334661

Strand: Direct

Name: miaB

Synonym: PsycPRwf_1875

Alternate gene names: 148653672

Gene position: 2333189-2334661 (Clockwise)

Preceding gene: 148653668

Following gene: 148653676

Centisome position: 78.32

GC content: 45.62

Gene sequence:

>1473_bases
ATGAGTGCTACTTTATTTGACCCCAAATCTATCGCTGTCTCAAATCCAGTCGATACCTCAACTCAAGCCAGCTTGGCGCA
TGCCAGCCAGCCTACGGGACAAGCCAAAAAGAAAGTTTATATTGCCACCCAAGGCTGTCAAATGAACGTTTATGATTCAG
AAAAAATGGGTAACGTTCTAGGCGACTCCCATGACATGGTGGTTACTGATAATATCGAAGAGGCCGATGTGTTGCTCATG
AACACCTGCTCCATTCGTGAAAAAGCCCAAGAAAAAGTATTCTCTGAGCTTGGCCGCTGGCGTAAGTTAAAAGAAGAAAA
ACCAGATTTGGTTATCGGTGTGGGCGGCTGCGTGGCCTCTCAAGAAGGTGACAATATCCAAAAAAGAGCCCCTTATGTGG
ATATGGTATTTGGCCCACAAACCTTACACAGATTGCCCGAGCTATATGATAAATCGACAACTCAGCGCAATGTCAAACCC
AAAGATCGTATTGGTACGGTCGATGTGTCATTCCCAAGTATTGAAAAATTTGACTTCTTGCCTGAGCCAAAAGTAGAAGG
CTACCGTGCCTTTGTTTCTATCATGGAAGGCTGCTCAAAGTACTGCTCATTTTGCGTCGTGCCTTATACCCGTGGTGAGG
AGCTGTCACGTCCTTTAGATGACGTATTGGCTGAGATTGATAGCCTCGCTGAGCAAGGCGTGCGCGAGATCACCTTATTG
GGCCAGAACGTCAATGGCTATCGCGGTGAAAAAGACGATGGCAGCATCTGCCGCTTTGCTGAGCTGCTACACTATGTGGC
CCACGTGGATGGTATTGAACGTATTCGTTACACCACCAGCCACCCGCTTGAATTCACTGATGACATCATTGAAGCTTATG
CTAAATTGCCGCAATTGGTATCGCATCTGCATCTTCCGGTACAAAGTGGCTCCAACAAAATCTTGGCGGCCATGAAACGC
AATCACACCATTGATGTTTATATCAATCAGATTAATAAGCTGATGGCTGTGCGCCCTGATATGCACTTATCAAGTGACTT
TATCATTGGCTTCCCTGGAGAGACAGAAGAGGACTTCTTAGACACCTTAAATTTAGCCAAAGCGTTAGATTTTGATCACT
CATATAGCTTTATCTACTCAAAACGTCCGGGCACACCGGCTTCAGAGTTACCAGACGATGTGAGCTTGGCGACCAAAAAG
GAGCGTTTGGCCATTTTCCAAAAAGTCATTCGTGATTCAACGCTTAAGAAAACCGAAGAAATGGTGGGCAAAACTCTACG
AGTAATGGTTGAGGAGATTGCCGATCGTTATCCCGATCAATTGCTGGGTACAGCAGACAACACACGCTCAGTACTGTTTA
AAGCCACTGAGCAGCAAAAAACAGACTTGATGGGTAAATTTGTGACGGTCAAAATCACTGACTTTGTAAGCCCGCATATG
GTACGTGGTGAGTTGGTGGATATCTTAGATTAG

Upstream 100 bases:

>100_bases
AAAAAGGCAACACCCTCTTTGCTTGATATATCTCGATAACGGTTCGTTGTATCTATCCTATACCTTCTTTTAATCCACAC
ATCACCTTGGACTGCTTAAT

Downstream 100 bases:

>100_bases
CTGTTGCTGCGCCGTATCCGCTCAGTAGCATAAAAAACAGCCCAATGACATCATTGGGCTGTTTTTTATGATGGTTAATA
CTTAGCTAGATATCCACGCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVLGDSHDMVVTDNIEEADVLLM
NTCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKP
KDRIGTVDVSFPSIEKFDFLPEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLL
GQNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLVSHLHLPVQSGSNKILAAMKR
NHTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFLDTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKK
ERLAIFQKVIRDSTLKKTEEMVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHM
VRGELVDILD

Sequences:

>Translated_490_residues
MSATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVLGDSHDMVVTDNIEEADVLLM
NTCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKP
KDRIGTVDVSFPSIEKFDFLPEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLL
GQNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLVSHLHLPVQSGSNKILAAMKR
NHTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFLDTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKK
ERLAIFQKVIRDSTLKKTEEMVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHM
VRGELVDILD
>Mature_489_residues
SATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVLGDSHDMVVTDNIEEADVLLMN
TCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKPK
DRIGTVDVSFPSIEKFDFLPEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLLG
QNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLVSHLHLPVQSGSNKILAAMKRN
HTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFLDTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKKE
RLAIFQKVIRDSTLKKTEEMVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHMV
RGELVDILD

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=487, Percent_Identity=34.9075975359343, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI28872784, Length=487, Percent_Identity=34.9075975359343, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI93277076, Length=410, Percent_Identity=27.3170731707317, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=59.1111111111111, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787057, Length=414, Percent_Identity=29.7101449275362, Blast_Score=165, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=32.7659574468085, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=291, Percent_Identity=28.8659793814433, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356207, Length=493, Percent_Identity=32.2515212981744, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI19922432, Length=476, Percent_Identity=26.4705882352941, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PSYWF (A5WGM4)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280765.1
- ProteinModelPortal:   A5WGM4
- SMR:   A5WGM4
- STRING:   A5WGM4
- GeneID:   5204696
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1875
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   PSP56811:PSYCPRWF_1875-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 54898; Mature: 54767

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVL
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHH
GDSHDMVVTDNIEEADVLLMNTCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVAS
CCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEC
QEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKPKDRIGTVDVSFPSIEKFDFL
CCCCCHHHHCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHCCCC
PEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLL
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEE
GQNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
SHLHLPVQSGSNKILAAMKRNHTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFL
HHHCCCCCCCCCCEEHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
DTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKKERLAIFQKVIRDSTLKKTEE
HHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHM
HHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHEEEHHHHHHHHHHCCEEEEEEECCCCCHH
VRGELVDILD
HHHHHHHHCC
>Mature Secondary Structure 
SATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVL
CCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHH
GDSHDMVVTDNIEEADVLLMNTCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVAS
CCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEC
QEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKPKDRIGTVDVSFPSIEKFDFL
CCCCCHHHHCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHCCCC
PEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLL
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEE
GQNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
SHLHLPVQSGSNKILAAMKRNHTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFL
HHHCCCCCCCCCCEEHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
DTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKKERLAIFQKVIRDSTLKKTEE
HHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHM
HHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHEEEHHHHHHHHHHCCEEEEEEECCCCCHH
VRGELVDILD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA