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The map label for this gene is rlmL

Identifier: 148653568

GI number: 148653568

Start: 2191977

End: 2194400

Strand: Direct

Name: rlmL

Synonym: PsycPRwf_1771

Alternate gene names: 148653568

Gene position: 2191977-2194400 (Clockwise)

Preceding gene: 148653567

Following gene: 148653571

Centisome position: 73.58

GC content: 46.29

Gene sequence:

>2424_bases
ATGTCCACCCCCGAAAACACCCTTGTAACACCTAACACATCAGACACCATACCGACGGCTTCCGCTGCCAAGCGCTATGA
TATTGTCGTAACCTGCGCTGATGGCCTAGAGGCACCGCTGCAGACTGAGCTTAGCCACATGGATATTAGCCATGAGCTAA
AAAGTACCGGCCGTATTGCGGTCAATGCCACCTTAGAGCAAATCTATAAAATCTGCTTGTGGTCACGCGTCGCCTCGCGC
GTGTTATTGCCGATCAAAAAGCGCAATATCAATAAAGAGTATGACGTCGCCGAACAGCTAAATGGTCTGGCGCGTACAGT
GGACTGGACTGAGTGGTTTGAGTTAGACAATACCTTTGCTATCCGAATGAGTGTCGATAAAAGGGTTCAAGTTAGCCAGC
AGTTTGCCATGCTGCGTATTAAAGATGCCATCGCTGATACTTTTAGCGAAAAGCTCGATGCCCGCCCTGATGTCGATAGC
AATAATCCTGACTTCCCTATCTATGCTACCGTCAATGAAAAACAGGCCGAGATTTTCTTAGATTTATCCGGAACCAGCCT
ACACCGCCGCGGTTACCGTGTGGCCATGACCGATGCCCCGCTTAAAGAAAACTTAGCGGCCGGCTTGTTATATACCGTAG
GCTGGCACAAAACCAAAAACAGCTATAGTGCGCTTATTGACCCCATGTGTGGCTCCGGTACGTTTATCATTGAAGCGCTA
TTAATGCACTGTGATTATCCGGTGGGCATTGATAAAGCCGAATCTCAATTTGGTTTTTATAACTGGCAAGAGCATGACAG
CGAGTTATGGCAGCAATGTGTGGTACAGGCCCAAGAGCGCTTCCATAATGGACTACAAAAAGCTGCAGCCGGTAAATTAC
CGACTATTTTAGGCTTCGATGCCGATGCTGGCGCCATAAAAGCGGTACATAAAAACTTAATCGCCGCAGGTTTAATTGAA
CTGATTCCGCATTTAACGCTAGAGCAGCGTCCGCTGTCACAGCTAAAGATAGCTTTGGCCAAACCGCTATTAGACCGTAA
GCTAAAACGTCCTTTGGTTATTACCAACCCGCCTTATGGCGAGCGTTTGGGCGAAAGTGACTTTATTAAGCCCTTGTATC
AAGGCCTAGGTTTGACCTTGCAAGAGATTTTCGCCAAACAAAAATACCAGCCCATGCTAGGTCTGCTTGCCGCTCATGTC
GAGCAAGCTGACGTGCTGCCTATTGAAGACCCGCAAACTTTGCGCTGTCATAATGGCGCCATTACCGTGTATTTCCGTCA
TGGTCAGCTGATCCGCAAAGAGGGCGAAAGCCTAATCACCCGCTTTGAGAAAACCGAAATTAAAGTGGATGAAGCGCAAG
ATTTCGTCAACCGTCTGCAAAAGAATGTGAACCATCTAAAAAAACTGGCTGCCAAAGAAGATGTGACCAACTTGCGTGTT
TATGATGCAGATTTGCCCGACTATAAAGTGGCCATTGATGTTTATGGCGACTATGTCCATGTGCAAGAGTGGGCTCCGCC
GAAGAGTATTCCACCAGAAACTGCTCGCAAACGCTTTAATCTTGCGCTCATGGGTATCCGTGAAGTGTTTGGCATCAACC
GAGAGCAAATCTTTATCAAGACCCGTGCTCGACAAGTGGGTAACACTCAATATGGCAACCCTGAAGCCAGCGCTCAATCT
AAAGAGTCAAAAAATGCCCCAGAGCCTAAAAAGGACAACCGCAATCGCTATAAAGGCAATAAGTTTCAGCAAGCCCGTGA
AGAGGCCAAGCGCCAAGAAGCTCAGCGTCTGGCACAAAAGAAACGTAAAATGCATGTGGTGCAAGAAGACGGCGCTTACT
TTTATGTTAACTTCACTGACTACCTAGATACTGGGCTGTTTATTGATCACCGCAATATGCGTAGTATGATTCGCTCTGCC
AGCCGCGGTGCGGATGTATTAAACCTATTCGCTTATACCTGTACTGCCAGTGTTCACGCCGCACTTGGCGGGGCAAAATC
AGTGACCAGTGTCGATTTATCACAAAACTATCTGGACTGGGGCAAACAAAACTTTGCGCTAAATGGTCTAGATGTGACCA
CCAGCCGTTATCAGTTTATTGCCTCTGATATCTTTGACTGGATTAAAGACAATACCGATCAGTTTGATGTGATATTTATT
GATCCACCAACGTTCTCAAACTCAAAGAAGTTCCAAGGGACATTTGATGTGCAACGTGACCACACAGCGCTGATTAACCG
TGCGATGAACCGTTTGGCTAATGGTGGTGTGATTTACTTCTCTAATAACTTTACTAAGTTTGAGTTAGATGAAGAGCTAT
ATGATCGTTATGAAGTCACTGAGATTACCAGTGAAACCATTGGTTTTGACTTTAATCCCAAAAAGCCTATTCACCACAGC
TTTGAGATTCGTCATAAGCTTTAA

Upstream 100 bases:

>100_bases
TTTTTACCGCGTACTATGGCAAGCGATCTGCTTGCCACCGCTAAAACAGTCTAAAACCTGTGATGATTGAATAAGTAACA
TACCAACGTAAAGGCAAATT

Downstream 100 bases:

>100_bases
AAGTTAGCGATAGTACTTTACACATAAACAAAGCCCCGTTAGTTAGCTAGCGGGGCTTTGTTTTGGATTGTCTTTATTTA
TTAGGTATACGTAGGTTTGC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSTPENTLVTPNTSDTIPTASAAKRYDIVVTCADGLEAPLQTELSHMDISHELKSTGRIAVNATLEQIYKICLWSRVASR
VLLPIKKRNINKEYDVAEQLNGLARTVDWTEWFELDNTFAIRMSVDKRVQVSQQFAMLRIKDAIADTFSEKLDARPDVDS
NNPDFPIYATVNEKQAEIFLDLSGTSLHRRGYRVAMTDAPLKENLAAGLLYTVGWHKTKNSYSALIDPMCGSGTFIIEAL
LMHCDYPVGIDKAESQFGFYNWQEHDSELWQQCVVQAQERFHNGLQKAAAGKLPTILGFDADAGAIKAVHKNLIAAGLIE
LIPHLTLEQRPLSQLKIALAKPLLDRKLKRPLVITNPPYGERLGESDFIKPLYQGLGLTLQEIFAKQKYQPMLGLLAAHV
EQADVLPIEDPQTLRCHNGAITVYFRHGQLIRKEGESLITRFEKTEIKVDEAQDFVNRLQKNVNHLKKLAAKEDVTNLRV
YDADLPDYKVAIDVYGDYVHVQEWAPPKSIPPETARKRFNLALMGIREVFGINREQIFIKTRARQVGNTQYGNPEASAQS
KESKNAPEPKKDNRNRYKGNKFQQAREEAKRQEAQRLAQKKRKMHVVQEDGAYFYVNFTDYLDTGLFIDHRNMRSMIRSA
SRGADVLNLFAYTCTASVHAALGGAKSVTSVDLSQNYLDWGKQNFALNGLDVTTSRYQFIASDIFDWIKDNTDQFDVIFI
DPPTFSNSKKFQGTFDVQRDHTALINRAMNRLANGGVIYFSNNFTKFELDEELYDRYEVTEITSETIGFDFNPKKPIHHS
FEIRHKL

Sequences:

>Translated_807_residues
MSTPENTLVTPNTSDTIPTASAAKRYDIVVTCADGLEAPLQTELSHMDISHELKSTGRIAVNATLEQIYKICLWSRVASR
VLLPIKKRNINKEYDVAEQLNGLARTVDWTEWFELDNTFAIRMSVDKRVQVSQQFAMLRIKDAIADTFSEKLDARPDVDS
NNPDFPIYATVNEKQAEIFLDLSGTSLHRRGYRVAMTDAPLKENLAAGLLYTVGWHKTKNSYSALIDPMCGSGTFIIEAL
LMHCDYPVGIDKAESQFGFYNWQEHDSELWQQCVVQAQERFHNGLQKAAAGKLPTILGFDADAGAIKAVHKNLIAAGLIE
LIPHLTLEQRPLSQLKIALAKPLLDRKLKRPLVITNPPYGERLGESDFIKPLYQGLGLTLQEIFAKQKYQPMLGLLAAHV
EQADVLPIEDPQTLRCHNGAITVYFRHGQLIRKEGESLITRFEKTEIKVDEAQDFVNRLQKNVNHLKKLAAKEDVTNLRV
YDADLPDYKVAIDVYGDYVHVQEWAPPKSIPPETARKRFNLALMGIREVFGINREQIFIKTRARQVGNTQYGNPEASAQS
KESKNAPEPKKDNRNRYKGNKFQQAREEAKRQEAQRLAQKKRKMHVVQEDGAYFYVNFTDYLDTGLFIDHRNMRSMIRSA
SRGADVLNLFAYTCTASVHAALGGAKSVTSVDLSQNYLDWGKQNFALNGLDVTTSRYQFIASDIFDWIKDNTDQFDVIFI
DPPTFSNSKKFQGTFDVQRDHTALINRAMNRLANGGVIYFSNNFTKFELDEELYDRYEVTEITSETIGFDFNPKKPIHHS
FEIRHKL
>Mature_806_residues
STPENTLVTPNTSDTIPTASAAKRYDIVVTCADGLEAPLQTELSHMDISHELKSTGRIAVNATLEQIYKICLWSRVASRV
LLPIKKRNINKEYDVAEQLNGLARTVDWTEWFELDNTFAIRMSVDKRVQVSQQFAMLRIKDAIADTFSEKLDARPDVDSN
NPDFPIYATVNEKQAEIFLDLSGTSLHRRGYRVAMTDAPLKENLAAGLLYTVGWHKTKNSYSALIDPMCGSGTFIIEALL
MHCDYPVGIDKAESQFGFYNWQEHDSELWQQCVVQAQERFHNGLQKAAAGKLPTILGFDADAGAIKAVHKNLIAAGLIEL
IPHLTLEQRPLSQLKIALAKPLLDRKLKRPLVITNPPYGERLGESDFIKPLYQGLGLTLQEIFAKQKYQPMLGLLAAHVE
QADVLPIEDPQTLRCHNGAITVYFRHGQLIRKEGESLITRFEKTEIKVDEAQDFVNRLQKNVNHLKKLAAKEDVTNLRVY
DADLPDYKVAIDVYGDYVHVQEWAPPKSIPPETARKRFNLALMGIREVFGINREQIFIKTRARQVGNTQYGNPEASAQSK
ESKNAPEPKKDNRNRYKGNKFQQAREEAKRQEAQRLAQKKRKMHVVQEDGAYFYVNFTDYLDTGLFIDHRNMRSMIRSAS
RGADVLNLFAYTCTASVHAALGGAKSVTSVDLSQNYLDWGKQNFALNGLDVTTSRYQFIASDIFDWIKDNTDQFDVIFID
PPTFSNSKKFQGTFDVQRDHTALINRAMNRLANGGVIYFSNNFTKFELDEELYDRYEVTEITSETIGFDFNPKKPIHHSF
EIRHKL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=738, Percent_Identity=35.5013550135501, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI145693111, Length=156, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_PSYWF (A5WGC0)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280661.1
- ProteinModelPortal:   A5WGC0
- SMR:   A5WGC0
- STRING:   A5WGC0
- GeneID:   5206179
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1771
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   PSP56811:PSYCPRWF_1771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 91671; Mature: 91540

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPENTLVTPNTSDTIPTASAAKRYDIVVTCADGLEAPLQTELSHMDISHELKSTGRIA
CCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHCCCCEE
VNATLEQIYKICLWSRVASRVLLPIKKRNINKEYDVAEQLNGLARTVDWTEWFELDNTFA
EEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCEEE
IRMSVDKRVQVSQQFAMLRIKDAIADTFSEKLDARPDVDSNNPDFPIYATVNEKQAEIFL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEE
DLSGTSLHRRGYRVAMTDAPLKENLAAGLLYTVGWHKTKNSYSALIDPMCGSGTFIIEAL
EECCCCHHHCCCEEEEECCCHHHHHHHHHHEEECCCCCCCCHHHHHCCCCCCCHHHHHHH
LMHCDYPVGIDKAESQFGFYNWQEHDSELWQQCVVQAQERFHNGLQKAAAGKLPTILGFD
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
ADAGAIKAVHKNLIAAGLIELIPHLTLEQRPLSQLKIALAKPLLDRKLKRPLVITNPPYG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCH
ERLGESDFIKPLYQGLGLTLQEIFAKQKYQPMLGLLAAHVEQADVLPIEDPQTLRCHNGA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCE
ITVYFRHGQLIRKEGESLITRFEKTEIKVDEAQDFVNRLQKNVNHLKKLAAKEDVTNLRV
EEEEEECCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YDADLPDYKVAIDVYGDYVHVQEWAPPKSIPPETARKRFNLALMGIREVFGINREQIFIK
EECCCCCEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCEEEEE
TRARQVGNTQYGNPEASAQSKESKNAPEPKKDNRNRYKGNKFQQAREEAKRQEAQRLAQK
EHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRKMHVVQEDGAYFYVNFTDYLDTGLFIDHRNMRSMIRSASRGADVLNLFAYTCTASVHA
HHHEEEEEECCCEEEEEEHHHHCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALGGAKSVTSVDLSQNYLDWGKQNFALNGLDVTTSRYQFIASDIFDWIKDNTDQFDVIFI
HHCCCCCEEEECCHHHHHHCCCCCEEECCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPPTFSNSKKFQGTFDVQRDHTALINRAMNRLANGGVIYFSNNFTKFELDEELYDRYEVT
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHCHHHHH
EITSETIGFDFNPKKPIHHSFEIRHKL
HHHHHHCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
STPENTLVTPNTSDTIPTASAAKRYDIVVTCADGLEAPLQTELSHMDISHELKSTGRIA
CCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHCCCCEE
VNATLEQIYKICLWSRVASRVLLPIKKRNINKEYDVAEQLNGLARTVDWTEWFELDNTFA
EEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCEEE
IRMSVDKRVQVSQQFAMLRIKDAIADTFSEKLDARPDVDSNNPDFPIYATVNEKQAEIFL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEE
DLSGTSLHRRGYRVAMTDAPLKENLAAGLLYTVGWHKTKNSYSALIDPMCGSGTFIIEAL
EECCCCHHHCCCEEEEECCCHHHHHHHHHHEEECCCCCCCCHHHHHCCCCCCCHHHHHHH
LMHCDYPVGIDKAESQFGFYNWQEHDSELWQQCVVQAQERFHNGLQKAAAGKLPTILGFD
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
ADAGAIKAVHKNLIAAGLIELIPHLTLEQRPLSQLKIALAKPLLDRKLKRPLVITNPPYG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCH
ERLGESDFIKPLYQGLGLTLQEIFAKQKYQPMLGLLAAHVEQADVLPIEDPQTLRCHNGA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCE
ITVYFRHGQLIRKEGESLITRFEKTEIKVDEAQDFVNRLQKNVNHLKKLAAKEDVTNLRV
EEEEEECCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YDADLPDYKVAIDVYGDYVHVQEWAPPKSIPPETARKRFNLALMGIREVFGINREQIFIK
EECCCCCEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCEEEEE
TRARQVGNTQYGNPEASAQSKESKNAPEPKKDNRNRYKGNKFQQAREEAKRQEAQRLAQK
EHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRKMHVVQEDGAYFYVNFTDYLDTGLFIDHRNMRSMIRSASRGADVLNLFAYTCTASVHA
HHHEEEEEECCCEEEEEEHHHHCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALGGAKSVTSVDLSQNYLDWGKQNFALNGLDVTTSRYQFIASDIFDWIKDNTDQFDVIFI
HHCCCCCEEEECCHHHHHHCCCCCEEECCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPPTFSNSKKFQGTFDVQRDHTALINRAMNRLANGGVIYFSNNFTKFELDEELYDRYEVT
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHCHHHHH
EITSETIGFDFNPKKPIHHSFEIRHKL
HHHHHHCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA