| Definition | Psychrobacter sp. PRwf-1 chromosome, complete genome. |
|---|---|
| Accession | NC_009524 |
| Length | 2,978,976 |
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The map label for this gene is mrcB [H]
Identifier: 148653418
GI number: 148653418
Start: 2003947
End: 2006592
Strand: Direct
Name: mrcB [H]
Synonym: PsycPRwf_1620
Alternate gene names: 148653418
Gene position: 2003947-2006592 (Clockwise)
Preceding gene: 148653416
Following gene: 148653419
Centisome position: 67.27
GC content: 47.54
Gene sequence:
>2646_bases ATGGGCATATATCGGCTATTAGATGGCAATTACATTGAAAAAAAGGCGGTTTTCAGTCATTATAATCGGGTCTTTTTTTA TGTTGGTAAAACTATCGTGCATCACAAACCCTCTCGCGCTTCTTCTCATTTTGACCAATCATCTGCACAATTCTCCAAGT CCTCGTTTAGCGAAGGCAAACTTCCCAACCCGCAGCAGCAACGCGGCTCAGTGATTGGCTTTTTTTTACTGATTATCATC ATTGTCGGTATGTTGATGCTGGCCATGTATTTGATCAAGCAAGACCGCGTTATTACTGCCAAATTTGAGGGCAAACGCTG GAACTTGCCAGCCAAGGTGTACTCGCAGCCTTTAGAGCTTTATCAAGGGGCAGCCCTCACCAACAAGAACTTAGATGCTT GGCTTGAGATGCTCAATTACAAATCCAGCTCTAATTACAACAATACTGGCACCTATAACAAGTCCTCCGGCCAGTATGTC ATTCATACCCGTGGCTTTCAGTACAGCGCCAATGATGTTGACCCTGAGCAAGTGATTAAGATTAAAATCCAAGACTCTAA AGTCACCAGCATTCAAAGTACCGAACCCAATGATACCGGTATTGTGCGTATTGAGCCGGTTACCATCGGTGGTATTTATC CAGATAACAATGAAGACCGCATCGTGTTGCCTATTGATGAGATTCCGCAGCCGTTGATTGATGCGCTTATCGCCACCGAA GACCGCGGCTTTTATGAGCACAAAGGCGTGTCGCTCAAGGGTATCGGCCGAGCACTGTATAACAACTTAAAAGGCGGCTC AATGCAAGGTGGCTCGACCATCACTCAGCAGCTGATTAAAAACTTTTATCTAAGCTCAGATCGCACCTTTAAGCGTAAAG CCAATGAGGCGGTGATGGCCTTGCTACTCGAGATGCACTACAGCAAAGACCAAATTTTACAAACTTATTTAAATGAGATT AACCTAGGTCAAAATGGCAACCGTTCTATTAACGGCTTTGGGTTGGCTGCGCAGTTTTATTTTAATAAACCGTTAAAAGA GCTGCGTATTGATCAGCAAGCCATGCTGGTGGGCTTAGCCAAAGGCACAAGCTATTACAATCCACGCCGCAATCCAGAGC GTGCCCTCAATCGCCGCAATACAGTGCTGGCCAATATGCTTGAAACGGGCAAAATTGATCAAGCCACTTATGATGACGCC ATTGCTCAAAAGCTTGATGTGGTACCTAAACCAAGCGTAGGCAAAAGCCGATTCCCCGACTTTTTGGACATCGTAAAGCG TGAGCTGCATGATAATTATCATCCCGATGATCTAAAAAATGAAGGCCTACGCGTCTTTAGTACCTTAGACCCTATCGCTC AGATGTCGGCTGATGCAGCGGTACAAAAGCAATTATCAGGCTTACGAAAAAGCAGCAGCAAAACCAAGGGGCTACAAGCG GCCTTGGTCAGTGCCAATCCAGCTTCAGGTGAGCTGATTGCTGCTGTGGGTAGTGGTAGCGAATTTACAGGCTTCAACCG AGCGATAGATGCCAAACGTCAAGTGGGCTCACTGTTAAAACCCTTTATTTATATGACGGCGCTACAAAGTGGCAAGTACA ATCTGGCCAGCCCTGTGAGCGACAACCCAGTTACGCTAACGTTACCAGACAACACCACTTGGACCCCAAAAAACTACGAT GGCCGAGACCATGGTTACGTGCCCTTAACCACAGCGCTGGCCAAATCTTATAACCAAGCTGCGGTAAATACGGGTATAGA ATTTGGTGTGGGCCCGTTCGTCGATCAGCTTCACCGCTTAGGTATTAAGGAGAAGATACCAACCTATCCTTCGTTGTTCT TAGGCTCAGTTGAGCTTAGTCCTATGGATATGCTTGGTATGTATCAAGTCATGGCCGCAGGTGGATTTCGCACGCCCATT ATTAGTATCCGCTCTGTGGTTGATGACCGAGGTCAAATTCTGCAGCGCTCAGGGCTAGATACCAAGCGCAGCGTGCCACC TGATGTCAACTATCTAACCAACTATGCGCTACAAGAAGTGGTGCGCAGTGGAACGGCAAGCTCGCTAAAATCCTTAGGCA GTGATCTAAATCTGGCCGGTAAAACAGGCACCACCAACGACTACCGCGACTCTTGGTTTGCCGGCTACAGTGGCAACTAT GTCAGCGTAGTATGGGTGGGCCGTGATGACAATAAGCCCATTGGTTTAAGTGGTGGCAGCGGTGCATTACCTGTGTGGAA GGACTTTATGGGTCGTCTGCGCTTGACCCCTGTCGAGCTGGTGCAGCCTGACAGTGTAGAGTGGTTATGGCTAGAAAATG GCACCGGAAAACTGTCGCATGAAAGCTGTGACAATGCCCGCTACTTGCCCGTACTTAGCAGATATTTGCCTGAAGAGGCC AGTGACTGCGCCCTTGCCATCTATGAGCAAGAGCAGGCACGCCAAAGACAGCAGTGGTTCAATGATCAGCGCAAAGCTTT ACTGGATCAAAGCCGTCAGCTCGATAGTGTTCAAGAAGGTATGCCCGCCAATACTGATTCAAGCGCTAATGATGATGCCT CTGCAGGCAATGCTCCTGAGCAAGAAGGCTCAGAGCCTACCAATAATGGTGATACTTGGTATGATAAAGCGTTACAATGG TTTTAA
Upstream 100 bases:
>100_bases TATGAGGGCGAAGTGTATTTTTGAAAGTTTGCGCTTGAATAAATCAGGCGCTTATGCAGGATGCCCTAACTCGTGAAAAA GCGATGAAAAGCTACTTTTA
Downstream 100 bases:
>100_bases TTGGCCTAAAAATTTTATCTCATAATTTGGATGTGCTTTATGCTTATGCCCGCTCCTACCCCAACCGTTTGCGCTAAGCG TCACAACTTTTTTATGGCAG
Product: penicillin-binding protein 1B
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 881; Mature: 880
Protein sequence:
>881_residues MGIYRLLDGNYIEKKAVFSHYNRVFFYVGKTIVHHKPSRASSHFDQSSAQFSKSSFSEGKLPNPQQQRGSVIGFFLLIII IVGMLMLAMYLIKQDRVITAKFEGKRWNLPAKVYSQPLELYQGAALTNKNLDAWLEMLNYKSSSNYNNTGTYNKSSGQYV IHTRGFQYSANDVDPEQVIKIKIQDSKVTSIQSTEPNDTGIVRIEPVTIGGIYPDNNEDRIVLPIDEIPQPLIDALIATE DRGFYEHKGVSLKGIGRALYNNLKGGSMQGGSTITQQLIKNFYLSSDRTFKRKANEAVMALLLEMHYSKDQILQTYLNEI NLGQNGNRSINGFGLAAQFYFNKPLKELRIDQQAMLVGLAKGTSYYNPRRNPERALNRRNTVLANMLETGKIDQATYDDA IAQKLDVVPKPSVGKSRFPDFLDIVKRELHDNYHPDDLKNEGLRVFSTLDPIAQMSADAAVQKQLSGLRKSSSKTKGLQA ALVSANPASGELIAAVGSGSEFTGFNRAIDAKRQVGSLLKPFIYMTALQSGKYNLASPVSDNPVTLTLPDNTTWTPKNYD GRDHGYVPLTTALAKSYNQAAVNTGIEFGVGPFVDQLHRLGIKEKIPTYPSLFLGSVELSPMDMLGMYQVMAAGGFRTPI ISIRSVVDDRGQILQRSGLDTKRSVPPDVNYLTNYALQEVVRSGTASSLKSLGSDLNLAGKTGTTNDYRDSWFAGYSGNY VSVVWVGRDDNKPIGLSGGSGALPVWKDFMGRLRLTPVELVQPDSVEWLWLENGTGKLSHESCDNARYLPVLSRYLPEEA SDCALAIYEQEQARQRQQWFNDQRKALLDQSRQLDSVQEGMPANTDSSANDDASAGNAPEQEGSEPTNNGDTWYDKALQW F
Sequences:
>Translated_881_residues MGIYRLLDGNYIEKKAVFSHYNRVFFYVGKTIVHHKPSRASSHFDQSSAQFSKSSFSEGKLPNPQQQRGSVIGFFLLIII IVGMLMLAMYLIKQDRVITAKFEGKRWNLPAKVYSQPLELYQGAALTNKNLDAWLEMLNYKSSSNYNNTGTYNKSSGQYV IHTRGFQYSANDVDPEQVIKIKIQDSKVTSIQSTEPNDTGIVRIEPVTIGGIYPDNNEDRIVLPIDEIPQPLIDALIATE DRGFYEHKGVSLKGIGRALYNNLKGGSMQGGSTITQQLIKNFYLSSDRTFKRKANEAVMALLLEMHYSKDQILQTYLNEI NLGQNGNRSINGFGLAAQFYFNKPLKELRIDQQAMLVGLAKGTSYYNPRRNPERALNRRNTVLANMLETGKIDQATYDDA IAQKLDVVPKPSVGKSRFPDFLDIVKRELHDNYHPDDLKNEGLRVFSTLDPIAQMSADAAVQKQLSGLRKSSSKTKGLQA ALVSANPASGELIAAVGSGSEFTGFNRAIDAKRQVGSLLKPFIYMTALQSGKYNLASPVSDNPVTLTLPDNTTWTPKNYD GRDHGYVPLTTALAKSYNQAAVNTGIEFGVGPFVDQLHRLGIKEKIPTYPSLFLGSVELSPMDMLGMYQVMAAGGFRTPI ISIRSVVDDRGQILQRSGLDTKRSVPPDVNYLTNYALQEVVRSGTASSLKSLGSDLNLAGKTGTTNDYRDSWFAGYSGNY VSVVWVGRDDNKPIGLSGGSGALPVWKDFMGRLRLTPVELVQPDSVEWLWLENGTGKLSHESCDNARYLPVLSRYLPEEA SDCALAIYEQEQARQRQQWFNDQRKALLDQSRQLDSVQEGMPANTDSSANDDASAGNAPEQEGSEPTNNGDTWYDKALQW F >Mature_880_residues GIYRLLDGNYIEKKAVFSHYNRVFFYVGKTIVHHKPSRASSHFDQSSAQFSKSSFSEGKLPNPQQQRGSVIGFFLLIIII VGMLMLAMYLIKQDRVITAKFEGKRWNLPAKVYSQPLELYQGAALTNKNLDAWLEMLNYKSSSNYNNTGTYNKSSGQYVI HTRGFQYSANDVDPEQVIKIKIQDSKVTSIQSTEPNDTGIVRIEPVTIGGIYPDNNEDRIVLPIDEIPQPLIDALIATED RGFYEHKGVSLKGIGRALYNNLKGGSMQGGSTITQQLIKNFYLSSDRTFKRKANEAVMALLLEMHYSKDQILQTYLNEIN LGQNGNRSINGFGLAAQFYFNKPLKELRIDQQAMLVGLAKGTSYYNPRRNPERALNRRNTVLANMLETGKIDQATYDDAI AQKLDVVPKPSVGKSRFPDFLDIVKRELHDNYHPDDLKNEGLRVFSTLDPIAQMSADAAVQKQLSGLRKSSSKTKGLQAA LVSANPASGELIAAVGSGSEFTGFNRAIDAKRQVGSLLKPFIYMTALQSGKYNLASPVSDNPVTLTLPDNTTWTPKNYDG RDHGYVPLTTALAKSYNQAAVNTGIEFGVGPFVDQLHRLGIKEKIPTYPSLFLGSVELSPMDMLGMYQVMAAGGFRTPII SIRSVVDDRGQILQRSGLDTKRSVPPDVNYLTNYALQEVVRSGTASSLKSLGSDLNLAGKTGTTNDYRDSWFAGYSGNYV SVVWVGRDDNKPIGLSGGSGALPVWKDFMGRLRLTPVELVQPDSVEWLWLENGTGKLSHESCDNARYLPVLSRYLPEEAS DCALAIYEQEQARQRQQWFNDQRKALLDQSRQLDSVQEGMPANTDSSANDDASAGNAPEQEGSEPTNNGDTWYDKALQWF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=702, Percent_Identity=39.031339031339, Blast_Score=478, Evalue=1e-135, Organism=Escherichia coli, GI87082258, Length=248, Percent_Identity=38.3064516129032, Blast_Score=163, Evalue=4e-41, Organism=Escherichia coli, GI1788867, Length=545, Percent_Identity=28.0733944954128, Blast_Score=154, Evalue=2e-38, Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=1e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 97819; Mature: 97688
Theoretical pI: Translated: 8.38; Mature: 8.38
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGIYRLLDGNYIEKKAVFSHYNRVFFYVGKTIVHHKPSRASSHFDQSSAQFSKSSFSEGK CCEEEECCCCCHHHHHHHHHHCEEEEEECHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCC LPNPQQQRGSVIGFFLLIIIIVGMLMLAMYLIKQDRVITAKFEGKRWNLPAKVYSQPLEL CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH YQGAALTNKNLDAWLEMLNYKSSSNYNNTGTYNKSSGQYVIHTRGFQYSANDVDPEQVIK HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCEEE IKIQDSKVTSIQSTEPNDTGIVRIEPVTIGGIYPDNNEDRIVLPIDEIPQPLIDALIATE EEEECCEECEEECCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHHHHHCC DRGFYEHKGVSLKGIGRALYNNLKGGSMQGGSTITQQLIKNFYLSSDRTFKRKANEAVMA CCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH LLLEMHYSKDQILQTYLNEINLGQNGNRSINGFGLAAQFYFNKPLKELRIDQQAMLVGLA HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHCCCHHHHHCCHHHHHEEHH KGTSYYNPRRNPERALNRRNTVLANMLETGKIDQATYDDAIAQKLDVVPKPSVGKSRFPD CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH FLDIVKRELHDNYHPDDLKNEGLRVFSTLDPIAQMSADAAVQKQLSGLRKSSSKTKGLQA HHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEE ALVSANPASGELIAAVGSGSEFTGFNRAIDAKRQVGSLLKPFIYMTALQSGKYNLASPVS EEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC DNPVTLTLPDNTTWTPKNYDGRDHGYVPLTTALAKSYNQAAVNTGIEFGVGPFVDQLHRL CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC GIKEKIPTYPSLFLGSVELSPMDMLGMYQVMAAGGFRTPIISIRSVVDDRGQILQRSGLD CCHHCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC TKRSVPPDVNYLTNYALQEVVRSGTASSLKSLGSDLNLAGKTGTTNDYRDSWFAGYSGNY CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCE VSVVWVGRDDNKPIGLSGGSGALPVWKDFMGRLRLTPVELVQPDSVEWLWLENGTGKLSH EEEEEEECCCCCCEECCCCCCCCHHHHHHHCCCCCCCHHHCCCCCEEEEEEECCCCCCCC ESCDNARYLPVLSRYLPEEASDCALAIYEQEQARQRQQWFNDQRKALLDQSRQLDSVQEG CCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC MPANTDSSANDDASAGNAPEQEGSEPTNNGDTWYDKALQWF CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCC >Mature Secondary Structure GIYRLLDGNYIEKKAVFSHYNRVFFYVGKTIVHHKPSRASSHFDQSSAQFSKSSFSEGK CEEEECCCCCHHHHHHHHHHCEEEEEECHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCC LPNPQQQRGSVIGFFLLIIIIVGMLMLAMYLIKQDRVITAKFEGKRWNLPAKVYSQPLEL CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH YQGAALTNKNLDAWLEMLNYKSSSNYNNTGTYNKSSGQYVIHTRGFQYSANDVDPEQVIK HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCEEE IKIQDSKVTSIQSTEPNDTGIVRIEPVTIGGIYPDNNEDRIVLPIDEIPQPLIDALIATE EEEECCEECEEECCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHHHHHCC DRGFYEHKGVSLKGIGRALYNNLKGGSMQGGSTITQQLIKNFYLSSDRTFKRKANEAVMA CCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH LLLEMHYSKDQILQTYLNEINLGQNGNRSINGFGLAAQFYFNKPLKELRIDQQAMLVGLA HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHCCCHHHHHCCHHHHHEEHH KGTSYYNPRRNPERALNRRNTVLANMLETGKIDQATYDDAIAQKLDVVPKPSVGKSRFPD CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH FLDIVKRELHDNYHPDDLKNEGLRVFSTLDPIAQMSADAAVQKQLSGLRKSSSKTKGLQA HHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEE ALVSANPASGELIAAVGSGSEFTGFNRAIDAKRQVGSLLKPFIYMTALQSGKYNLASPVS EEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC DNPVTLTLPDNTTWTPKNYDGRDHGYVPLTTALAKSYNQAAVNTGIEFGVGPFVDQLHRL CCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC GIKEKIPTYPSLFLGSVELSPMDMLGMYQVMAAGGFRTPIISIRSVVDDRGQILQRSGLD CCHHCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC TKRSVPPDVNYLTNYALQEVVRSGTASSLKSLGSDLNLAGKTGTTNDYRDSWFAGYSGNY CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCE VSVVWVGRDDNKPIGLSGGSGALPVWKDFMGRLRLTPVELVQPDSVEWLWLENGTGKLSH EEEEEEECCCCCCEECCCCCCCCHHHHHHHCCCCCCCHHHCCCCCEEEEEEECCCCCCCC ESCDNARYLPVLSRYLPEEASDCALAIYEQEQARQRQQWFNDQRKALLDQSRQLDSVQEG CCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC MPANTDSSANDDASAGNAPEQEGSEPTNNGDTWYDKALQWF CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]