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The map label for this gene is nirB [H]

Identifier: 148653350

GI number: 148653350

Start: 1902246

End: 1904888

Strand: Direct

Name: nirB [H]

Synonym: PsycPRwf_1552

Alternate gene names: 148653350

Gene position: 1902246-1904888 (Clockwise)

Preceding gene: 148653344

Following gene: 148653351

Centisome position: 63.86

GC content: 43.85

Gene sequence:

>2643_bases
ATGATTAAGGCAAATATGAGTAATATAAATAGTAAACAAAAAAACAAACCTAACCTTGTTGTGGTCGGTAACGGCATGGT
GGGGCATCACTTTGTAGAGAAGGCCATTGAACTTGGCTTAAACACTACCTATGACATTCACGTCTTTGCTGAAGAAGATC
GCCCAGCTTATGATCGTGTCTATTTATCAAGCTATTTTGAACATAAAGATGCCACCAAGCTAAACCTAGTGGATATGGCT
GACTATGATGCCACTGGGATTCAGCTGCATCTAAATAAGAAAATTGTAGATATCGACAGCGAGGCTCATACCCTGACCGT
AGAAGGCGGCGAAAGTGTTGAGTATAAAAATCTAGTTTTAGCAACCGGCTCATACCCGTTTGTGCCCCCTATTGAAGGTG
GTGATCATGCTCATTGTCATGTCTATCGTACCATTGCTGATTTGGATGATATTCTTGAAACCGCCCAAAAACCCAGTGCT
AAAAAAGGGGTGGTCGTCGGTGGCGGGTTGTTAGGGCTAGAGGCTGCCAAAGCATTGGTAACCTTGGGGCTTGATACTTA
CGTGGTTGAATTTGCCCCTCAGTTGATGGGTGTTCAGTTAGACCCTGCTGGTGGTGAAATCCTAAAGAAGAAGATTGAAG
CATTGGGCGTTACCGTATTAACGGGAAAAAACACCAAAGAAATTGTTCATAACCGCAGTGTTTCCTTACACCATGACATC
CATCAAAAAGACAGTCAAAATGAGGTGCATCTGGAAAAAGATACTTTCTTAACCATGAAGTTTACAGATGATACCGAACT
AGATACCGATTTAATCTTATTTAGTGCCGGCATTCGTCCGCAAGATGGCATTATGAGAAGTAATCCTGAGTGCGGAGTCA
CTTTAGGAGGCCGTGGTGGCATCTTAATCAATGATCATTGTCAGACCAATAATGAAGATATCTATGCTATCGGTGAATGT
GCAGTTTGGGACAATAAAATCTTTGGTCTGGTCGCCCCTGGCTATGCCATGGCCAGTATATGTGCTGCTCAAATAGCGGG
TGACACTGAGCAAACCTTTACTGGCGCAGATATGAGCACCAAGCTTAAATTAATGGGTGTTGATGTGGGCAGTATTGGCG
ACGCTCATGCCACAACTGAGGGCAGCCTAAGCTACATTTATCAAAATCCCAATGAAGGCATCTATAAAAAGCTGGTTACC
AGTGGCGATAATAAAAAGCTGCTGGGCGCTGTCATGGTGGGTGATACCGAAGACTACAATAATTTATTGCAGTTATTCTT
AAATGACATCGACCTTCCCGCTCATCCAGAGAGCTTGATTTTACCCAATGTTGAAAAACCCTCAATGGGCATTGAAAATC
TTCCTGATACAGCCACCATTTGCTCTTGTTTTAATGTATCTAAAGGTGCCATTTGTGGTGCGGTTGAAGACGGGGCTTAT
TCACTGGCAGATGTCAAAAGCTGCACCAAAGCAGGTACAGGCTGTGGCGGCTGTGCCCCTATGGTGAAAGACGTGCTGGC
TTATCAATTAACCGACCTTGGTTTTGAGGTCAATAATGATCTGTGTGAGCATTTTTCTTATTCTCGCCAAGAGTTATATG
GCTTAGTACGTGTTAAAGGCATCAAAACCTTTGATGATTTATTAACACAGCACGGCACAGGCCGTGGTTGTGAGATTTGC
AAACCTGCAGTGGCTTCCATTTTATCGTCTTGTTGGAATGACTATGTCCTTAAAACAGAGCTTGCGCCGCTGCAAGAAAC
CAACGATTACTACTTAGGCAATATCCAAAAGGATGGCACCTACTCTGTTGTGCCGCGTGTGGCTGGCGGCGAAATTACCG
CTGATAAACTTATCGTATTGGGTCAAGTGGCCAAAGACTTTAACCTTTATACCAAAATTACCGGTGGTCAACGGGTTGAT
TTATTTGGGGCACGGTTAGAACAATTGCCTGATATTTGGGAGTGCTTAGTAGAGGCAGGCTTCGAAACTGGCCATGCTTA
TGGTAAATCACTGCGTACTGTTAAGTCATGTGTTGGTGAGAACTGGTGTCGTTATGGTGTCGCCGATAGCGTCGGCTTAG
CCTTAACTTTAGAGAACCGATACAAGGGAGTGCGCTCACCGCATAAAATCAAAATGGGTGTTTCCGGTTGTACCCGAGAG
TGTGCCGAAGCCCAATCAAAAGACTTTGGGGTTATTGCTACCGAAAATGGCTGGAATTTATACGTGGCCGGTAATGGTGG
CATGGTACCTCGTCATGGAGAGCTATTCGCCACCGATTTAGACACTGAGACCTTAATCAAATATATCGACCGTATCACGA
TGTTTTACATCAAAACCGCGGATAAGTTACAGCGCACCTCAAAATGGCGTGAAAGCTTAGAAGGCGGATTAGAGTATTTA
CAACAAGTTATTATCGAAGATAGCTTAGATATTGCTCAAGAGCTTGAAGATCAAATGCAGTTGTTGGTTGATAATTATGT
CTGCGAATGGAAAGAAACCATTACTGACAAAGAGAAGTTAAAACGCTTCCGTCATTTTGTTAACAGCGAGCTAGGTGATG
ACAATGTGGTATTTGTGACTGAACGTGAACAAATTCGCCCTGCGACTGAGCTCGAAAAAGAAGCGGTAGAGGCCGTGGTT
TAA

Upstream 100 bases:

>100_bases
TCTGGCATGATTATTGAATGTATTACGGTAGGCACTAAGTGGCTTTCACTTTAATAAGTGATTAAAAATAGCTACTAGAC
ACCCACTAAAGGACACATTC

Downstream 100 bases:

>100_bases
TCATCCTAATTACACCCAAGCCACTATTTTTTATCCAGCTTTTATCCAGCTTTTTATGCAACTGTTACGCTCACCCGACT
AAAAAAGGACGTCATTATGA

Product: nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MIKANMSNINSKQKNKPNLVVVGNGMVGHHFVEKAIELGLNTTYDIHVFAEEDRPAYDRVYLSSYFEHKDATKLNLVDMA
DYDATGIQLHLNKKIVDIDSEAHTLTVEGGESVEYKNLVLATGSYPFVPPIEGGDHAHCHVYRTIADLDDILETAQKPSA
KKGVVVGGGLLGLEAAKALVTLGLDTYVVEFAPQLMGVQLDPAGGEILKKKIEALGVTVLTGKNTKEIVHNRSVSLHHDI
HQKDSQNEVHLEKDTFLTMKFTDDTELDTDLILFSAGIRPQDGIMRSNPECGVTLGGRGGILINDHCQTNNEDIYAIGEC
AVWDNKIFGLVAPGYAMASICAAQIAGDTEQTFTGADMSTKLKLMGVDVGSIGDAHATTEGSLSYIYQNPNEGIYKKLVT
SGDNKKLLGAVMVGDTEDYNNLLQLFLNDIDLPAHPESLILPNVEKPSMGIENLPDTATICSCFNVSKGAICGAVEDGAY
SLADVKSCTKAGTGCGGCAPMVKDVLAYQLTDLGFEVNNDLCEHFSYSRQELYGLVRVKGIKTFDDLLTQHGTGRGCEIC
KPAVASILSSCWNDYVLKTELAPLQETNDYYLGNIQKDGTYSVVPRVAGGEITADKLIVLGQVAKDFNLYTKITGGQRVD
LFGARLEQLPDIWECLVEAGFETGHAYGKSLRTVKSCVGENWCRYGVADSVGLALTLENRYKGVRSPHKIKMGVSGCTRE
CAEAQSKDFGVIATENGWNLYVAGNGGMVPRHGELFATDLDTETLIKYIDRITMFYIKTADKLQRTSKWRESLEGGLEYL
QQVIIEDSLDIAQELEDQMQLLVDNYVCEWKETITDKEKLKRFRHFVNSELGDDNVVFVTEREQIRPATELEKEAVEAVV

Sequences:

>Translated_880_residues
MIKANMSNINSKQKNKPNLVVVGNGMVGHHFVEKAIELGLNTTYDIHVFAEEDRPAYDRVYLSSYFEHKDATKLNLVDMA
DYDATGIQLHLNKKIVDIDSEAHTLTVEGGESVEYKNLVLATGSYPFVPPIEGGDHAHCHVYRTIADLDDILETAQKPSA
KKGVVVGGGLLGLEAAKALVTLGLDTYVVEFAPQLMGVQLDPAGGEILKKKIEALGVTVLTGKNTKEIVHNRSVSLHHDI
HQKDSQNEVHLEKDTFLTMKFTDDTELDTDLILFSAGIRPQDGIMRSNPECGVTLGGRGGILINDHCQTNNEDIYAIGEC
AVWDNKIFGLVAPGYAMASICAAQIAGDTEQTFTGADMSTKLKLMGVDVGSIGDAHATTEGSLSYIYQNPNEGIYKKLVT
SGDNKKLLGAVMVGDTEDYNNLLQLFLNDIDLPAHPESLILPNVEKPSMGIENLPDTATICSCFNVSKGAICGAVEDGAY
SLADVKSCTKAGTGCGGCAPMVKDVLAYQLTDLGFEVNNDLCEHFSYSRQELYGLVRVKGIKTFDDLLTQHGTGRGCEIC
KPAVASILSSCWNDYVLKTELAPLQETNDYYLGNIQKDGTYSVVPRVAGGEITADKLIVLGQVAKDFNLYTKITGGQRVD
LFGARLEQLPDIWECLVEAGFETGHAYGKSLRTVKSCVGENWCRYGVADSVGLALTLENRYKGVRSPHKIKMGVSGCTRE
CAEAQSKDFGVIATENGWNLYVAGNGGMVPRHGELFATDLDTETLIKYIDRITMFYIKTADKLQRTSKWRESLEGGLEYL
QQVIIEDSLDIAQELEDQMQLLVDNYVCEWKETITDKEKLKRFRHFVNSELGDDNVVFVTEREQIRPATELEKEAVEAVV
>Mature_880_residues
MIKANMSNINSKQKNKPNLVVVGNGMVGHHFVEKAIELGLNTTYDIHVFAEEDRPAYDRVYLSSYFEHKDATKLNLVDMA
DYDATGIQLHLNKKIVDIDSEAHTLTVEGGESVEYKNLVLATGSYPFVPPIEGGDHAHCHVYRTIADLDDILETAQKPSA
KKGVVVGGGLLGLEAAKALVTLGLDTYVVEFAPQLMGVQLDPAGGEILKKKIEALGVTVLTGKNTKEIVHNRSVSLHHDI
HQKDSQNEVHLEKDTFLTMKFTDDTELDTDLILFSAGIRPQDGIMRSNPECGVTLGGRGGILINDHCQTNNEDIYAIGEC
AVWDNKIFGLVAPGYAMASICAAQIAGDTEQTFTGADMSTKLKLMGVDVGSIGDAHATTEGSLSYIYQNPNEGIYKKLVT
SGDNKKLLGAVMVGDTEDYNNLLQLFLNDIDLPAHPESLILPNVEKPSMGIENLPDTATICSCFNVSKGAICGAVEDGAY
SLADVKSCTKAGTGCGGCAPMVKDVLAYQLTDLGFEVNNDLCEHFSYSRQELYGLVRVKGIKTFDDLLTQHGTGRGCEIC
KPAVASILSSCWNDYVLKTELAPLQETNDYYLGNIQKDGTYSVVPRVAGGEITADKLIVLGQVAKDFNLYTKITGGQRVD
LFGARLEQLPDIWECLVEAGFETGHAYGKSLRTVKSCVGENWCRYGVADSVGLALTLENRYKGVRSPHKIKMGVSGCTRE
CAEAQSKDFGVIATENGWNLYVAGNGGMVPRHGELFATDLDTETLIKYIDRITMFYIKTADKLQRTSKWRESLEGGLEYL
QQVIIEDSLDIAQELEDQMQLLVDNYVCEWKETITDKEKLKRFRHFVNSELGDDNVVFVTEREQIRPATELEKEAVEAVV

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=868, Percent_Identity=55.9907834101382, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=337, Percent_Identity=23.4421364985163, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788892, Length=305, Percent_Identity=22.2950819672131, Blast_Score=76, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=318, Percent_Identity=25.1572327044025, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 96446; Mature: 96446

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKANMSNINSKQKNKPNLVVVGNGMVGHHFVEKAIELGLNTTYDIHVFAEEDRPAYDRV
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHH
YLSSYFEHKDATKLNLVDMADYDATGIQLHLNKKIVDIDSEAHTLTVEGGESVEYKNLVL
HHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEECEEEE
ATGSYPFVPPIEGGDHAHCHVYRTIADLDDILETAQKPSAKKGVVVGGGLLGLEAAKALV
EECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHH
TLGLDTYVVEFAPQLMGVQLDPAGGEILKKKIEALGVTVLTGKNTKEIVHNRSVSLHHDI
HHCCHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHCEEEEECCCHHHHHHCCCCEEEEHH
HQKDSQNEVHLEKDTFLTMKFTDDTELDTDLILFSAGIRPQDGIMRSNPECGVTLGGRGG
CCCCCCCCEEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCC
ILINDHCQTNNEDIYAIGECAVWDNKIFGLVAPGYAMASICAAQIAGDTEQTFTGADMST
EEEECCCCCCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCC
KLKLMGVDVGSIGDAHATTEGSLSYIYQNPNEGIYKKLVTSGDNKKLLGAVMVGDTEDYN
EEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCHH
NLLQLFLNDIDLPAHPESLILPNVEKPSMGIENLPDTATICSCFNVSKGAICGAVEDGAY
HHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCC
SLADVKSCTKAGTGCGGCAPMVKDVLAYQLTDLGFEVNNDLCEHFSYSRQELYGLVRVKG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEECCHHHHHHHCCCHHHHHHHHEECC
IKTFDDLLTQHGTGRGCEICKPAVASILSSCWNDYVLKTELAPLQETNDYYLGNIQKDGT
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCC
YSVVPRVAGGEITADKLIVLGQVAKDFNLYTKITGGQRVDLFGARLEQLPDIWECLVEAG
EEEECCCCCCCCCHHHEEEEEHHHHCCEEEEEECCCCEEEHHHHHHHHCHHHHHHHHHCC
FETGHAYGKSLRTVKSCVGENWCRYGVADSVGLALTLENRYKGVRSPHKIKMGVSGCTRE
CCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECHHHCCCCCCCEEEECCCHHHHH
CAEAQSKDFGVIATENGWNLYVAGNGGMVPRHGELFATDLDTETLIKYIDRITMFYIKTA
HHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHEEECCH
DKLQRTSKWRESLEGGLEYLQQVIIEDSLDIAQELEDQMQLLVDNYVCEWKETITDKEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KRFRHFVNSELGDDNVVFVTEREQIRPATELEKEAVEAVV
HHHHHHHCCCCCCCCEEEEECHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKANMSNINSKQKNKPNLVVVGNGMVGHHFVEKAIELGLNTTYDIHVFAEEDRPAYDRV
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHH
YLSSYFEHKDATKLNLVDMADYDATGIQLHLNKKIVDIDSEAHTLTVEGGESVEYKNLVL
HHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEECEEEE
ATGSYPFVPPIEGGDHAHCHVYRTIADLDDILETAQKPSAKKGVVVGGGLLGLEAAKALV
EECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHH
TLGLDTYVVEFAPQLMGVQLDPAGGEILKKKIEALGVTVLTGKNTKEIVHNRSVSLHHDI
HHCCHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHCEEEEECCCHHHHHHCCCCEEEEHH
HQKDSQNEVHLEKDTFLTMKFTDDTELDTDLILFSAGIRPQDGIMRSNPECGVTLGGRGG
CCCCCCCCEEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCC
ILINDHCQTNNEDIYAIGECAVWDNKIFGLVAPGYAMASICAAQIAGDTEQTFTGADMST
EEEECCCCCCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCC
KLKLMGVDVGSIGDAHATTEGSLSYIYQNPNEGIYKKLVTSGDNKKLLGAVMVGDTEDYN
EEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEECCCCCHH
NLLQLFLNDIDLPAHPESLILPNVEKPSMGIENLPDTATICSCFNVSKGAICGAVEDGAY
HHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCC
SLADVKSCTKAGTGCGGCAPMVKDVLAYQLTDLGFEVNNDLCEHFSYSRQELYGLVRVKG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEECCHHHHHHHCCCHHHHHHHHEECC
IKTFDDLLTQHGTGRGCEICKPAVASILSSCWNDYVLKTELAPLQETNDYYLGNIQKDGT
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCC
YSVVPRVAGGEITADKLIVLGQVAKDFNLYTKITGGQRVDLFGARLEQLPDIWECLVEAG
EEEECCCCCCCCCHHHEEEEEHHHHCCEEEEEECCCCEEEHHHHHHHHCHHHHHHHHHCC
FETGHAYGKSLRTVKSCVGENWCRYGVADSVGLALTLENRYKGVRSPHKIKMGVSGCTRE
CCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECHHHCCCCCCCEEEECCCHHHHH
CAEAQSKDFGVIATENGWNLYVAGNGGMVPRHGELFATDLDTETLIKYIDRITMFYIKTA
HHHHCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHEEECCH
DKLQRTSKWRESLEGGLEYLQQVIIEDSLDIAQELEDQMQLLVDNYVCEWKETITDKEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KRFRHFVNSELGDDNVVFVTEREQIRPATELEKEAVEAVV
HHHHHHHCCCCCCCCEEEEECHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2543955; 2200672; 9278503; 2445993 [H]