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The map label for this gene is potB [H]

Identifier: 148652869

GI number: 148652869

Start: 1282413

End: 1283339

Strand: Direct

Name: potB [H]

Synonym: PsycPRwf_1062

Alternate gene names: 148652869

Gene position: 1282413-1283339 (Clockwise)

Preceding gene: 148652868

Following gene: 148652870

Centisome position: 43.05

GC content: 44.12

Gene sequence:

>927_bases
ATGGCTGGGAGTGGATTTTACCTGATGAATAAATCAACATTATCTAATGCCATTAGTCACAGCAAAAATAATGGCCGCCT
ATTTCAGCGGGTCACCTTATTTGTAATTTGGGCCTGGCTGATTATTTTTGCATTATTGCCCAATATATTGGTGGTACTGG
CAAGCTTTTTAACCCGCGATGAAGATAAGTTCTTGGCAATGCCATTAACGGTTGAGAACTACTTACGCTTCATCGACCCA
TTGTACGTCAAAGTTTTTTTGCATTCGTTAAGTATGGCGGCCATTACCACCTTGATTTGTCTGCTGTTAGGCTATCCTTT
TGCATGGCTGATTAGCCGAGTATCAAAAAAATGGCAGTCGTTTTTAATGCTATTGTTAATTATTCCATTTTGGACCAACT
CATTGGTGCGCTTGTATGCTGTAAAACTGATTTTGGCGGCCAACGGGCTGTTAAGCTCAGCACTGATTGGAGCTGGGCTG
ATTAACGAGCCACTACAGATTTTATATACCCAAAAGGCGGTCATCGGCGGACTGGTGTATCTGTTATTTCCATTTATGGT
ATTGCCGCTGTATGCAGTATTTACCGATTTGCGAGACGACATTGTACTGGCTTCAAAGGACTTGGGCGCCAATAAGTTTC
AGACCTTTTGGCACGTTATATTGCCATTGACCACGCCCGGTATTATCTCAGGGGTGCTGCTGGTATTACTGCCGGCGATG
GGGATGTTCTATGTGGCTGATATCTTAGGCGGTTCACGTAACTTATTGGTGGGTAATATTATTAAGTCGCAGTTTTTGGA
TGCACGCGACTGGCCGTTTGGCGCTGCAGCGAGTGTGCTGTTGACCTTAACGATGGCAATATTGATTGCCGCTTATTACG
CCAGCAGCAAACGCATTGGCAAAACTGACCATAATGAAGTGGCATAG

Upstream 100 bases:

>100_bases
CTGCAAAACGGCAATGTGGTTAAGGCTTCTGAGTATTTCAACGAAGATGATCCCAATTTTAACTATAAATTGGGCCAAGA
GGTGCGTGTAGACTGGGTTG

Downstream 100 bases:

>100_bases
GGGAGTTGTATGAGTAGTTCAATGAAGTGGCTGTCTCGCCTCTATTTAACCCTAATGTATTTGATTATGTTTGCGCCAAT
TATGGTCATGGTGGTGTTCT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAGSGFYLMNKSTLSNAISHSKNNGRLFQRVTLFVIWAWLIIFALLPNILVVLASFLTRDEDKFLAMPLTVENYLRFIDP
LYVKVFLHSLSMAAITTLICLLLGYPFAWLISRVSKKWQSFLMLLLIIPFWTNSLVRLYAVKLILAANGLLSSALIGAGL
INEPLQILYTQKAVIGGLVYLLFPFMVLPLYAVFTDLRDDIVLASKDLGANKFQTFWHVILPLTTPGIISGVLLVLLPAM
GMFYVADILGGSRNLLVGNIIKSQFLDARDWPFGAAASVLLTLTMAILIAAYYASSKRIGKTDHNEVA

Sequences:

>Translated_308_residues
MAGSGFYLMNKSTLSNAISHSKNNGRLFQRVTLFVIWAWLIIFALLPNILVVLASFLTRDEDKFLAMPLTVENYLRFIDP
LYVKVFLHSLSMAAITTLICLLLGYPFAWLISRVSKKWQSFLMLLLIIPFWTNSLVRLYAVKLILAANGLLSSALIGAGL
INEPLQILYTQKAVIGGLVYLLFPFMVLPLYAVFTDLRDDIVLASKDLGANKFQTFWHVILPLTTPGIISGVLLVLLPAM
GMFYVADILGGSRNLLVGNIIKSQFLDARDWPFGAAASVLLTLTMAILIAAYYASSKRIGKTDHNEVA
>Mature_307_residues
AGSGFYLMNKSTLSNAISHSKNNGRLFQRVTLFVIWAWLIIFALLPNILVVLASFLTRDEDKFLAMPLTVENYLRFIDPL
YVKVFLHSLSMAAITTLICLLLGYPFAWLISRVSKKWQSFLMLLLIIPFWTNSLVRLYAVKLILAANGLLSSALIGAGLI
NEPLQILYTQKAVIGGLVYLLFPFMVLPLYAVFTDLRDDIVLASKDLGANKFQTFWHVILPLTTPGIISGVLLVLLPAMG
MFYVADILGGSRNLLVGNIIKSQFLDARDWPFGAAASVLLTLTMAILIAAYYASSKRIGKTDHNEVA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=271, Percent_Identity=51.6605166051661, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=39.9159663865546, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787713, Length=264, Percent_Identity=32.5757575757576, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34180; Mature: 34049

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSGFYLMNKSTLSNAISHSKNNGRLFQRVTLFVIWAWLIIFALLPNILVVLASFLTRD
CCCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EDKFLAMPLTVENYLRFIDPLYVKVFLHSLSMAAITTLICLLLGYPFAWLISRVSKKWQS
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLMLLLIIPFWTNSLVRLYAVKLILAANGLLSSALIGAGLINEPLQILYTQKAVIGGLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLFPFMVLPLYAVFTDLRDDIVLASKDLGANKFQTFWHVILPLTTPGIISGVLLVLLPAM
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GMFYVADILGGSRNLLVGNIIKSQFLDARDWPFGAAASVLLTLTMAILIAAYYASSKRIG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KTDHNEVA
CCCCCCCC
>Mature Secondary Structure 
AGSGFYLMNKSTLSNAISHSKNNGRLFQRVTLFVIWAWLIIFALLPNILVVLASFLTRD
CCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EDKFLAMPLTVENYLRFIDPLYVKVFLHSLSMAAITTLICLLLGYPFAWLISRVSKKWQS
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLMLLLIIPFWTNSLVRLYAVKLILAANGLLSSALIGAGLINEPLQILYTQKAVIGGLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLFPFMVLPLYAVFTDLRDDIVLASKDLGANKFQTFWHVILPLTTPGIISGVLLVLLPAM
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GMFYVADILGGSRNLLVGNIIKSQFLDARDWPFGAAASVLLTLTMAILIAAYYASSKRIG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KTDHNEVA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]