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The map label for this gene is dusB [H]

Identifier: 148652760

GI number: 148652760

Start: 1139270

End: 1140304

Strand: Direct

Name: dusB [H]

Synonym: PsycPRwf_0953

Alternate gene names: 148652760

Gene position: 1139270-1140304 (Clockwise)

Preceding gene: 148652759

Following gene: 148652761

Centisome position: 38.24

GC content: 48.21

Gene sequence:

>1035_bases
ATGGCAAATGATATGACATTACCCACCACCTTGGCCGATCATCCCTTATTGCAGCCATTATATATTGGCGGATTAGAGAT
TCAGAACCGATTGATGGTGGCGCCTATGGCTGGAGTAACAGACAACCCGTTTCGACGCTTATGCAAGTCGTTTGGGGCCG
GTCATGCCGTCAGCGAAATGATGACGGCTGATGCCACGTTATATGCCCATAAAAAGTCGTTATTTCGAGCCAACTTTGAG
GGAGAAATTGCTCCCATATCCGCGCAAATTGCAGGCGCAGATGTTGATAAAATTGCTGAAGCTGCTCAGTATCAGGTCAA
TAATGGGGCGCAGATTATTGATATTAATATGGGCTGCCCGGCCAAGAAGGTGTGCCGTAAACTGGCAGGCTCTGCTTTAT
TAAAAGATGAAGATCTGGTGGCACGTTTGCTGGATGCGACAGTGGCCGCAGTGGATGCACCTGTCACTTTAAAGACACGC
TTGGGCTATGAAAATGGACAGGAGAACATCTTGCGTGTGGCCAAACGAGCAGAAGAGGCGGGCATTGCTGCCATTGCTAT
CCACGGCCGTACTCGAGAAGACAAATACGGAGGTCAGGCCCGTTATGATCTCATTCGTGAAGTAAAGCAATTGGTTAACA
TCCCGGTGATTGCCAATGGCGACATTGACAGCCCCCAAAAAGCCATAGAAGTGTATGAGCAGACCGGCTGTGATGCCATT
ATGATTGGTCGTGCTGCTCAAGGTCAGCCTTGGCTGTTTCGAGATATCGCCCATTATTTGCAGACCGGTGAGATATTACC
AGCCCCCAGTATTGAGCAGTTAAGACAAATTGTATTGACTCATTTACAAGAGCTATATGATTTTTATGGTGAATACTCAG
GCTGCCGCATCGCCCGCAAGCACATTGCTTGGTATACCTCTGGTATCGAGGATTCTAACGCCTTTAGGCAGGCCATGTAT
GGCGAGGAGAGCACTGCTGGTCAATATAAAGTGGTGGATAAGTTTCTATCCTCTCATTCAGCTCGTGAACGCTAG

Upstream 100 bases:

>100_bases
TTACAATAACGGCAGGATAAAACGGGCTTGTCCTTATTAGGGCAAGCCTTTTTGTCTGCAAATAATATACTTCTTTTAAA
ATGATTTTTTGAGCGATTCA

Downstream 100 bases:

>100_bases
ACCGGCATCAGTACAGACCAACTTTGGCTAGGATTCACCGCTCAAATGGGTTAAATTAGGCGTTCAGTCTTTGTTATGAG
AATGAGTCCATGCCCGTAAG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MANDMTLPTTLADHPLLQPLYIGGLEIQNRLMVAPMAGVTDNPFRRLCKSFGAGHAVSEMMTADATLYAHKKSLFRANFE
GEIAPISAQIAGADVDKIAEAAQYQVNNGAQIIDINMGCPAKKVCRKLAGSALLKDEDLVARLLDATVAAVDAPVTLKTR
LGYENGQENILRVAKRAEEAGIAAIAIHGRTREDKYGGQARYDLIREVKQLVNIPVIANGDIDSPQKAIEVYEQTGCDAI
MIGRAAQGQPWLFRDIAHYLQTGEILPAPSIEQLRQIVLTHLQELYDFYGEYSGCRIARKHIAWYTSGIEDSNAFRQAMY
GEESTAGQYKVVDKFLSSHSARER

Sequences:

>Translated_344_residues
MANDMTLPTTLADHPLLQPLYIGGLEIQNRLMVAPMAGVTDNPFRRLCKSFGAGHAVSEMMTADATLYAHKKSLFRANFE
GEIAPISAQIAGADVDKIAEAAQYQVNNGAQIIDINMGCPAKKVCRKLAGSALLKDEDLVARLLDATVAAVDAPVTLKTR
LGYENGQENILRVAKRAEEAGIAAIAIHGRTREDKYGGQARYDLIREVKQLVNIPVIANGDIDSPQKAIEVYEQTGCDAI
MIGRAAQGQPWLFRDIAHYLQTGEILPAPSIEQLRQIVLTHLQELYDFYGEYSGCRIARKHIAWYTSGIEDSNAFRQAMY
GEESTAGQYKVVDKFLSSHSARER
>Mature_343_residues
ANDMTLPTTLADHPLLQPLYIGGLEIQNRLMVAPMAGVTDNPFRRLCKSFGAGHAVSEMMTADATLYAHKKSLFRANFEG
EIAPISAQIAGADVDKIAEAAQYQVNNGAQIIDINMGCPAKKVCRKLAGSALLKDEDLVARLLDATVAAVDAPVTLKTRL
GYENGQENILRVAKRAEEAGIAAIAIHGRTREDKYGGQARYDLIREVKQLVNIPVIANGDIDSPQKAIEVYEQTGCDAIM
IGRAAQGQPWLFRDIAHYLQTGEILPAPSIEQLRQIVLTHLQELYDFYGEYSGCRIARKHIAWYTSGIEDSNAFRQAMYG
EESTAGQYKVVDKFLSSHSARER

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=253, Percent_Identity=31.2252964426877, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI239788483, Length=236, Percent_Identity=31.3559322033898, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=28.695652173913, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=49.2211838006231, Blast_Score=317, Evalue=5e-88,
Organism=Escherichia coli, GI1788462, Length=257, Percent_Identity=32.295719844358, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=237, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=251, Percent_Identity=32.6693227091633, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=32.8767123287671, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=279, Percent_Identity=27.2401433691756, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=261, Percent_Identity=28.735632183908, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=26.9736842105263, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=25.5506607929515, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=280, Percent_Identity=27.5, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=269, Percent_Identity=30.8550185873606, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19920448, Length=269, Percent_Identity=30.8550185873606, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19921524, Length=251, Percent_Identity=29.0836653386454, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45549423, Length=254, Percent_Identity=29.9212598425197, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37796; Mature: 37664

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDMTLPTTLADHPLLQPLYIGGLEIQNRLMVAPMAGVTDNPFRRLCKSFGAGHAVSEM
CCCCCCCCCHHCCCCCCCHHEECCEECCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
MTADATLYAHKKSLFRANFEGEIAPISAQIAGADVDKIAEAAQYQVNNGAQIIDINMGCP
HHHHHHHHHHHHHHHHCCCCCCEEEEEHEECCCCHHHHHHHHHHEECCCCEEEEECCCCC
AKKVCRKLAGSALLKDEDLVARLLDATVAAVDAPVTLKTRLGYENGQENILRVAKRAEEA
HHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHC
GIAAIAIHGRTREDKYGGQARYDLIREVKQLVNIPVIANGDIDSPQKAIEVYEQTGCDAI
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEE
MIGRAAQGQPWLFRDIAHYLQTGEILPAPSIEQLRQIVLTHLQELYDFYGEYSGCRIARK
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
HIAWYTSGIEDSNAFRQAMYGEESTAGQYKVVDKFLSSHSARER
HHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ANDMTLPTTLADHPLLQPLYIGGLEIQNRLMVAPMAGVTDNPFRRLCKSFGAGHAVSEM
CCCCCCCCHHCCCCCCCHHEECCEECCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
MTADATLYAHKKSLFRANFEGEIAPISAQIAGADVDKIAEAAQYQVNNGAQIIDINMGCP
HHHHHHHHHHHHHHHHCCCCCCEEEEEHEECCCCHHHHHHHHHHEECCCCEEEEECCCCC
AKKVCRKLAGSALLKDEDLVARLLDATVAAVDAPVTLKTRLGYENGQENILRVAKRAEEA
HHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHC
GIAAIAIHGRTREDKYGGQARYDLIREVKQLVNIPVIANGDIDSPQKAIEVYEQTGCDAI
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEE
MIGRAAQGQPWLFRDIAHYLQTGEILPAPSIEQLRQIVLTHLQELYDFYGEYSGCRIARK
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
HIAWYTSGIEDSNAFRQAMYGEESTAGQYKVVDKFLSSHSARER
HHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]