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The map label for this gene is trpA

Identifier: 148652565

GI number: 148652565

Start: 901445

End: 902272

Strand: Direct

Name: trpA

Synonym: PsycPRwf_0756

Alternate gene names: 148652565

Gene position: 901445-902272 (Clockwise)

Preceding gene: 148652564

Following gene: 148652566

Centisome position: 30.26

GC content: 49.4

Gene sequence:

>828_bases
ATGACTCGAATTGAAAGCACCTTTGAGACCTTAAAAGCGCAAAACAAAAAAGCCCTAATCCCTTATGTTATGGCGGGTGA
CCCTTCCCCAAATAGCTTCGTGGACTTACTGCATGATTTAGTAAAACATGGGGCCGATATGATTGAAGTGGGATTGCCAT
TTTCAGACCCAATGGCGGATGGCCCGACTGTGGCATTGGCTGGTGAGCGCGCCTTAGCAGGCGGTACCAGTACCCATCAG
GCGCTGGCTATAGTCAAAAAATTCCGTGAACAAGACAGCAGCACGCCCATTATCTTAATGGGTTATCTCAATCCGATTGA
GATTATCGGTTATGAGGCCTTTATTGCCTTGTGTCACGAATCAGGGGTCGATGGGGTGTTGGTCGTGGATCTACCGCCTG
CTGAATCAGGTAATTTTGTTGAGCGCTTGGCGGCTCATGATATAAATAAGATTTTCTTATTATCACCGACCACCTTGCCT
GAGCGCCGTAAGCAAGTAATGACCCACTGCGGCGGCTATATTTATTATGTATCGCTTAAAGGGGTGACCGGCTCAGCAAG
CTTAGATACCCAAGCGGTCGGTGAGCAGGTGCAGGCCATCAAGCAAGAAACCAATTTACCCATCTGTGTCGGCTTTGGTA
TCCGCGATGGGCAGTCTGCTGCGGCCATTGGTCAGTATGCAGATGGGGTGATTGTGGGCAGTGAGCTGGTGAAGAACTTT
AGCGGACTGAGCCACACTTCACGTGCTGAGGATATCGCCACTGCTCAAGCCAGCATAATGACCAAAATGGATGAACTGCG
TCAAGCGTTAGACGCCCTAAGTGCTTAA

Upstream 100 bases:

>100_bases
TAATGAAGGCTGAAGGCATTGAGCTTTAAGCCAATTATTTAATACAGCGGGCGTGTTATAGGCCCGCAGCAGTTATCACC
TTATATGTTAAGAGACCATC

Downstream 100 bases:

>100_bases
TGATAACCGCGAAACGTCTTTGAGCGCCAAACCGCTAAGTGTTTGGCGTTATTTACGCCATTTGAAAACCTGATATTTGT
TGAGATTTTGTTGACGTTAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTRIESTFETLKAQNKKALIPYVMAGDPSPNSFVDLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAGGTSTHQ
ALAIVKKFREQDSSTPIILMGYLNPIEIIGYEAFIALCHESGVDGVLVVDLPPAESGNFVERLAAHDINKIFLLSPTTLP
ERRKQVMTHCGGYIYYVSLKGVTGSASLDTQAVGEQVQAIKQETNLPICVGFGIRDGQSAAAIGQYADGVIVGSELVKNF
SGLSHTSRAEDIATAQASIMTKMDELRQALDALSA

Sequences:

>Translated_275_residues
MTRIESTFETLKAQNKKALIPYVMAGDPSPNSFVDLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAGGTSTHQ
ALAIVKKFREQDSSTPIILMGYLNPIEIIGYEAFIALCHESGVDGVLVVDLPPAESGNFVERLAAHDINKIFLLSPTTLP
ERRKQVMTHCGGYIYYVSLKGVTGSASLDTQAVGEQVQAIKQETNLPICVGFGIRDGQSAAAIGQYADGVIVGSELVKNF
SGLSHTSRAEDIATAQASIMTKMDELRQALDALSA
>Mature_274_residues
TRIESTFETLKAQNKKALIPYVMAGDPSPNSFVDLLHDLVKHGADMIEVGLPFSDPMADGPTVALAGERALAGGTSTHQA
LAIVKKFREQDSSTPIILMGYLNPIEIIGYEAFIALCHESGVDGVLVVDLPPAESGNFVERLAAHDINKIFLLSPTTLPE
RRKQVMTHCGGYIYYVSLKGVTGSASLDTQAVGEQVQAIKQETNLPICVGFGIRDGQSAAAIGQYADGVIVGSELVKNFS
GLSHTSRAEDIATAQASIMTKMDELRQALDALSA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=214, Percent_Identity=35.5140186915888, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=236, Percent_Identity=35.5932203389831, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PSYWF (A5WDG7)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279658.1
- ProteinModelPortal:   A5WDG7
- SMR:   A5WDG7
- STRING:   A5WDG7
- GeneID:   5205743
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0756
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   PSP56811:PSYCPRWF_0756-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29303; Mature: 29172

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIESTFETLKAQNKKALIPYVMAGDPSPNSFVDLLHDLVKHGADMIEVGLPFSDPMAD
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTVALAGERALAGGTSTHQALAIVKKFREQDSSTPIILMGYLNPIEIIGYEAFIALCHE
CCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGVDGVLVVDLPPAESGNFVERLAAHDINKIFLLSPTTLPERRKQVMTHCGGYIYYVSLK
CCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEE
GVTGSASLDTQAVGEQVQAIKQETNLPICVGFGIRDGQSAAAIGQYADGVIVGSELVKNF
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCEEEHHHHHHHH
SGLSHTSRAEDIATAQASIMTKMDELRQALDALSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRIESTFETLKAQNKKALIPYVMAGDPSPNSFVDLLHDLVKHGADMIEVGLPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTVALAGERALAGGTSTHQALAIVKKFREQDSSTPIILMGYLNPIEIIGYEAFIALCHE
CCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGVDGVLVVDLPPAESGNFVERLAAHDINKIFLLSPTTLPERRKQVMTHCGGYIYYVSLK
CCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEE
GVTGSASLDTQAVGEQVQAIKQETNLPICVGFGIRDGQSAAAIGQYADGVIVGSELVKNF
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCEEEHHHHHHHH
SGLSHTSRAEDIATAQASIMTKMDELRQALDALSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA