Click here to switch to the map view.

The map label for this gene is mdlC [H]

Identifier: 148652552

GI number: 148652552

Start: 885386

End: 887041

Strand: Direct

Name: mdlC [H]

Synonym: PsycPRwf_0743

Alternate gene names: 148652552

Gene position: 885386-887041 (Clockwise)

Preceding gene: 148652551

Following gene: 148652553

Centisome position: 29.72

GC content: 48.79

Gene sequence:

>1656_bases
ATGCAACACGATTCAATAACCCCCTTATCAAAAAAGACGTCTATGTTAGATACCACAGCGGAGTCTGTGGTATCGCAGAC
CGTGCAGCAGGTGGTGTTTGAGTTAATGCGCACGCTTAACATGACCACCGTTTTTGGTAATCCTGGGTCCACTGAACTGA
ATTTTCTGACCAACTTTCCTGAAGACTTCTCTTATGTATTAGGCTTGCATGAAGCTTCAGTGGTCGGTATGGCAGATGGT
TATGCTCAGGCTACAGGTAATGCCGCTTTTGTTAATCTACATTCGGCTGCAGGTGTGGGCAATGCGTTAGGCAATATTTT
TACCGCCTACCGCAACCACACCCCGTTAGTCATTACAGCAGGGCAGCAAGCCCGTAGCTTATTACCCTTTGCTCCGTATT
TGGGCGCTGAGCAAGCTGCACAGTTTCCACAGCCTTATATTAAATGGAGTATTGAGCCTGCCCGCGCGGAAGATGTGCCG
CTAGCTATTGCTCAGGCATATTTAATCGCCATGCAGCACCCACAGGGCCCGACATTTGTGTCGATTCCGTCCGATGATTG
GGACAAGCCTGCGGTATTGCCACTGCTATCACAGTCTTGTGGCCATAGTATTCCAAGCCCAGATGCGTTGGCTGAGCTGG
TAGAGGTTATGTCAACCAGTCAGAATATGGCACTGGTTGTTGGCTCAGATGTTGATCGCCAAGGGGGTTTTGAGTTGGCG
GTTTCTGTCGCAGAAGCGTGTCAAGCTCCTGTGTGGGAAGCGCCAAACTCCAGTCGAGCAAGTTTTCCTGAAAACCATCC
TTTATTTGCCGGATTCTTACCTGCAATTCCTGAGAAGCTGAGCGAAAAGCTACTTGGCTATGATACGATTGTGGTGATAG
GGGCACCTGCTTTTACTTTACATGTTGCAGGGACGCTCTCGCTCAAAAAGAGCAAGATTTATCAGCTTACCGATGATCCA
CAGTATGCGGCTCAATCAGTGGCAACAAAAACCTTATCAGGCAATATACGCGATAGCCTACAAGCGCTATTAGACAAATT
GCCCACGAGTATGACACCTCGCTCAGGGCTTGATTTGCCGGTTCGTAAGCCTGCCGCTGAGGTTCAGGGCAGCAATCCAA
TTAGCATTGAGTACGTGATGGCGACATTGGCGAAATATTGCCCAGAAGATGTCGTTATCGTTGAAGAGGCTCCCTCACAC
CGTCCTGCGATTCAGCGTTATTTGCCCATTACTCAGCCTAAGAGTTTTTATACGATGGCAAGCGGGGGACTGGGTTATGG
GTTACCGGCTGCCGTTGGGGTTGCTTTGGGCACCCAGCGTCGCACCCTATGTCTCATTGGTGATGGCTCCTCCATGTATT
CTATCCAAGCCATCTGGACCGCCGTACAGCATAACTTACCGGTTACGGTCATTGTGTTGAATAATACAGGCTATGGGGCG
ATGCGCTCGTTTAGTAAGATTATGGGCAGCACGCAAGTACCAGGGTTAGACCTACCTAATATTAATTTTGTTCAGCTGGC
ACAAAGTATGGGATGCCAAGCGCAAAAAGTAACAGACTATTCTGTGCTTGATAAAGTGTTCGCTGATACCATGCAAGCAG
CGGGTAGTTATTTGCTAGAGATTATGGTCGATGCCAATACAGGCGCTGTCTATTAG

Upstream 100 bases:

>100_bases
TTATCGAATACAAGCACATCGTCATGCAGCCTGGTGTCGCAGGTACGTCAGCCGATTAGTATTTAGCTACTTCTTCGCGT
TGTTACATAAGGAAATGATA

Downstream 100 bases:

>100_bases
AGTTGGCAAGACTTGTCACCCTAGGGCAGCAGTTTGCAATACCAGTGCTAACAATACTAACACTCTCGTCAGTACTGACA
TCCTGATGAGGCTTACGATA

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MQHDSITPLSKKTSMLDTTAESVVSQTVQQVVFELMRTLNMTTVFGNPGSTELNFLTNFPEDFSYVLGLHEASVVGMADG
YAQATGNAAFVNLHSAAGVGNALGNIFTAYRNHTPLVITAGQQARSLLPFAPYLGAEQAAQFPQPYIKWSIEPARAEDVP
LAIAQAYLIAMQHPQGPTFVSIPSDDWDKPAVLPLLSQSCGHSIPSPDALAELVEVMSTSQNMALVVGSDVDRQGGFELA
VSVAEACQAPVWEAPNSSRASFPENHPLFAGFLPAIPEKLSEKLLGYDTIVVIGAPAFTLHVAGTLSLKKSKIYQLTDDP
QYAAQSVATKTLSGNIRDSLQALLDKLPTSMTPRSGLDLPVRKPAAEVQGSNPISIEYVMATLAKYCPEDVVIVEEAPSH
RPAIQRYLPITQPKSFYTMASGGLGYGLPAAVGVALGTQRRTLCLIGDGSSMYSIQAIWTAVQHNLPVTVIVLNNTGYGA
MRSFSKIMGSTQVPGLDLPNINFVQLAQSMGCQAQKVTDYSVLDKVFADTMQAAGSYLLEIMVDANTGAVY

Sequences:

>Translated_551_residues
MQHDSITPLSKKTSMLDTTAESVVSQTVQQVVFELMRTLNMTTVFGNPGSTELNFLTNFPEDFSYVLGLHEASVVGMADG
YAQATGNAAFVNLHSAAGVGNALGNIFTAYRNHTPLVITAGQQARSLLPFAPYLGAEQAAQFPQPYIKWSIEPARAEDVP
LAIAQAYLIAMQHPQGPTFVSIPSDDWDKPAVLPLLSQSCGHSIPSPDALAELVEVMSTSQNMALVVGSDVDRQGGFELA
VSVAEACQAPVWEAPNSSRASFPENHPLFAGFLPAIPEKLSEKLLGYDTIVVIGAPAFTLHVAGTLSLKKSKIYQLTDDP
QYAAQSVATKTLSGNIRDSLQALLDKLPTSMTPRSGLDLPVRKPAAEVQGSNPISIEYVMATLAKYCPEDVVIVEEAPSH
RPAIQRYLPITQPKSFYTMASGGLGYGLPAAVGVALGTQRRTLCLIGDGSSMYSIQAIWTAVQHNLPVTVIVLNNTGYGA
MRSFSKIMGSTQVPGLDLPNINFVQLAQSMGCQAQKVTDYSVLDKVFADTMQAAGSYLLEIMVDANTGAVY
>Mature_551_residues
MQHDSITPLSKKTSMLDTTAESVVSQTVQQVVFELMRTLNMTTVFGNPGSTELNFLTNFPEDFSYVLGLHEASVVGMADG
YAQATGNAAFVNLHSAAGVGNALGNIFTAYRNHTPLVITAGQQARSLLPFAPYLGAEQAAQFPQPYIKWSIEPARAEDVP
LAIAQAYLIAMQHPQGPTFVSIPSDDWDKPAVLPLLSQSCGHSIPSPDALAELVEVMSTSQNMALVVGSDVDRQGGFELA
VSVAEACQAPVWEAPNSSRASFPENHPLFAGFLPAIPEKLSEKLLGYDTIVVIGAPAFTLHVAGTLSLKKSKIYQLTDDP
QYAAQSVATKTLSGNIRDSLQALLDKLPTSMTPRSGLDLPVRKPAAEVQGSNPISIEYVMATLAKYCPEDVVIVEEAPSH
RPAIQRYLPITQPKSFYTMASGGLGYGLPAAVGVALGTQRRTLCLIGDGSSMYSIQAIWTAVQHNLPVTVIVLNNTGYGA
MRSFSKIMGSTQVPGLDLPNINFVQLAQSMGCQAQKVTDYSVLDKVFADTMQAAGSYLLEIMVDANTGAVY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=528, Percent_Identity=26.7045454545455, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790104, Length=535, Percent_Identity=22.4299065420561, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786717, Length=525, Percent_Identity=22.6666666666667, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=22.6860254083485, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=24.7440273037543, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=481, Percent_Identity=24.3243243243243, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=481, Percent_Identity=24.5322245322245, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=482, Percent_Identity=23.6514522821577, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 58877; Mature: 58877

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHDSITPLSKKTSMLDTTAESVVSQTVQQVVFELMRTLNMTTVFGNPGSTELNFLTNFP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCC
EDFSYVLGLHEASVVGMADGYAQATGNAAFVNLHSAAGVGNALGNIFTAYRNHTPLVITA
HHHHHHHHHHHHHEEECCCCCHHCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCEEEEC
GQQARSLLPFAPYLGAEQAAQFPQPYIKWSIEPARAEDVPLAIAQAYLIAMQHPQGPTFV
CHHHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCEEE
SIPSDDWDKPAVLPLLSQSCGHSIPSPDALAELVEVMSTSQNMALVVGSDVDRQGGFELA
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
VSVAEACQAPVWEAPNSSRASFPENHPLFAGFLPAIPEKLSEKLLGYDTIVVIGAPAFTL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEE
HVAGTLSLKKSKIYQLTDDPQYAAQSVATKTLSGNIRDSLQALLDKLPTSMTPRSGLDLP
EEEEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VRKPAAEVQGSNPISIEYVMATLAKYCPEDVVIVEEAPSHRPAIQRYLPITQPKSFYTMA
CCCCHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEEE
SGGLGYGLPAAVGVALGTQRRTLCLIGDGSSMYSIQAIWTAVQHNLPVTVIVLNNTGYGA
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEECCCCCHH
MRSFSKIMGSTQVPGLDLPNINFVQLAQSMGCQAQKVTDYSVLDKVFADTMQAAGSYLLE
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHEEEE
IMVDANTGAVY
EEEECCCCCCC
>Mature Secondary Structure
MQHDSITPLSKKTSMLDTTAESVVSQTVQQVVFELMRTLNMTTVFGNPGSTELNFLTNFP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCC
EDFSYVLGLHEASVVGMADGYAQATGNAAFVNLHSAAGVGNALGNIFTAYRNHTPLVITA
HHHHHHHHHHHHHEEECCCCCHHCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCEEEEC
GQQARSLLPFAPYLGAEQAAQFPQPYIKWSIEPARAEDVPLAIAQAYLIAMQHPQGPTFV
CHHHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCEEE
SIPSDDWDKPAVLPLLSQSCGHSIPSPDALAELVEVMSTSQNMALVVGSDVDRQGGFELA
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
VSVAEACQAPVWEAPNSSRASFPENHPLFAGFLPAIPEKLSEKLLGYDTIVVIGAPAFTL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEE
HVAGTLSLKKSKIYQLTDDPQYAAQSVATKTLSGNIRDSLQALLDKLPTSMTPRSGLDLP
EEEEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VRKPAAEVQGSNPISIEYVMATLAKYCPEDVVIVEEAPSHRPAIQRYLPITQPKSFYTMA
CCCCHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEEE
SGGLGYGLPAAVGVALGTQRRTLCLIGDGSSMYSIQAIWTAVQHNLPVTVIVLNNTGYGA
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEECCCCCHH
MRSFSKIMGSTQVPGLDLPNINFVQLAQSMGCQAQKVTDYSVLDKVFADTMQAAGSYLLE
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHEEEE
IMVDANTGAVY
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]